Exogenous CaCl2 reduces salt stress in sour jujube by reducing Na+ and increasing K+, Ca2+, and Mg2+ in different plant organs

In this study, we investigated the effects of 10 mM CaCl₂ on membrane permeability, malondialdehyde (MDA), reactive oxygen species (ROS), and Na⁺, K⁺, Ca²⁺, and Mg²⁺ concentrations in different organs of sour jujube grown under 150 mM NaCl stress. Our results showed 73% leaf wilting in the Na treatment. The Na treatment significantly increased leaf superoxide (O₂^.?) production rates, hydrogen peroxide (H₂O₂) concentrations, cell membrane permeability, MDA concentrations, and Na⁺ concentrations in roots, stems, and leaves. The Na treatment significantly reduced K⁺, Ca²⁺,and Mg²⁺ concentrations in roots, but increased K⁺ concentrations in stems. Compared with the Na treatment, 39% of the leaves showed wilting symptoms in the Na+Ca treatment. The Na+Ca treatment significantly decreased leaf O₂^.? production rates, H₂O₂ concentrations, cell membrane permeability, and MDA concentrations. Moreover, the Na+Ca treatment (1) significantly reduced Na⁺ concentrations in roots, stems, and leaves; (2) significantly increased root K⁺ concentrations; (3) significantly increased K⁺/Na⁺ ratios in roots; (4) significantly increased Ca²⁺ concentrations in stems and leaves, and Mg²⁺ concentrations in roots. In conclusion, exogenous CaCl₂ reduces NaCl stress in sour jujube by reducing Na+ concentrations and increasing K⁺, Ca²⁺, and Mg²⁺ concentrations in various plant organs.     

菜薹蛋白精氨酸甲基转移酶基因BrcuPRMT5的克隆及表达分析

以‘油青49’和‘油青甜菜薹80’菜薹茎尖为材料,采用RT-PCR和RACE技术克隆获得组蛋白甲基转移酶基因Brcu PRMT5的全长cDNA和gDNA序列。BrcuPRMT5 cDNA序列全长为2 117 bp,其中完整开放阅读框为1 929 bp,编码642个氨基酸,相对分子量为71.55 kD,理论等电点(p I)为5.87;多序列比对结果表明,Brcu PRMT5编码的氨基酸序列含有高等植物PRMT5基因1个高度保守的结构域;系统发育分析结果显示与大白菜、油菜及甘蓝的亲缘关系最近;亚细胞定位软件分析得知,Brcu PRMT5蛋白无跨膜区域,可能定位于线粒体中;对应的gDNA全长为4 151 bp,含有23个外显子和22个内含子,最长的外显子长度为140 bp,内含子的长度范围为50~150 bp。利用半定量RT-PCR和实时荧光定量PCR技术分析基因的表达,BrcuPRMT5在菜薹不同组织中均有表达,其中在花中表达量最高,叶次之,根中最低;BrcuPRMT5从苗期至完全抽薹开花期的表达量呈现上升趋势。BrcuPRMT5在菜薹花发育过程中可能起一定的调控作用。     

振动筛式红枣多层除杂分级机的设计与试验

为解决红枣收获后的除杂分级作业问题,设计了一种振动筛式红枣多层除杂分级机。运用Auto CAD和Solid Works对整机及关键部件进行设计,利用正交试验确定该型红枣除杂分级机的各项结构参数。试验结果表明:在作业效率为142kg/h、风选除杂装置的风速为14m/s、振动式除杂分级装置的倾斜角度为11°、振动筛的振动频率为20Hz时,除杂分级作业后的红枣含杂率为1.53%、分级合格率为94.8%、损伤率为3.1%。该机工作效率高,作业质量稳定,可同时完成收获后红枣杂质的去除与红枣尺寸的分级作业。     

荞麦剥壳效果在线检测装置的设计与试验

为提高荞麦剥壳设备的自动化程度,改善以往依靠手工筛分、计算的方法得到碎米率、整米率和未剥壳率,且不能实时检测等问题,设计了一种基于图像识别技术的荞麦剥壳效果在线检测装置并进行了测试试验。该装置主要由取样机构、匀样机构、图像识别系统和气流除尘光照箱等组成。试验结果表明:碎米率均值误差为1.0 8%、整米率均值误差为-4.4 9%、未剥壳率均值误差为3.4 3%,能够较为准确地反映出荞麦加工过程中的剥壳效果。     

1ZL5型马铃薯中耕机的设计与试验

针对马铃薯垄作栽培种植中肥量需求量大、土壤疏松程度高等要求,研究设计了1ZL5型马铃薯中耕机。通过对整机结构和工作原理的阐述以及对开沟铲和覆土铧工作断面参数的理论分析,获得了机具关键部件的具体结构参数。同时,对整机的作业性能进行了田间试验,结果表明:设计的马铃薯中耕机各项试验指标均满足马铃薯中耕要求,能够一次性完成松土、除草、筑垄等作业,还兼有蓄水、保墒的作用。该研究为马铃薯中耕机的研发提供了参考。     

立秆式搂膜机搂膜齿的设计与有限元分析

为了解决新疆棉花秋收作业后的残膜回收难题,设计了适用于新疆棉花种植模式的立秆式搂膜机。介绍了整机的基本结构和工作原理,运用相关理论对关键部件搂膜齿进行受力分析,并利用ANSYS对3种不同形式的搂膜齿在作业过程中的总变形和等效应力进行有限元分析。结果表明:单个搂膜齿在搂膜作业时承受水平方向的土壤阻力约为28.8~43.2N,竖直方向的土壤支持力约为47.4N;弯齿形式的搂膜齿作业过程中的最大总变形和等效应力最小,分别为34.61mm和340.29MPa;直齿与斜齿形式的搂膜齿作业过程中的最大总变形和等效应力值相接近,分别为65.67、68.28mm和462.06、472.28MPa。研究结果可为搂膜式残膜回收机的设计与改进提供参考。     

Validation of dual-crop coefficient method for calculation of rice evapotranspiration under drying–wetting cycle condition

Based on data collected from rice fields under drying–wetting cycle condition, the procedure of dual-crop coefficient (K _cd) approaches was calibrated and validated to reveal its feasibility and improve its performance in rice evapotranspiration (ET _c) estimation. It was found that K _cd based on FAO-recommended basal crop coefficients (K _cb) underestimated dual-crop coefficients in monsoon climate region in East China. The recommended coefficient (K _cp) value of 1.2 was not high enough to reflect the pulse increase of rice ET _c after soil wetting. The K _cb values were calibrated as 1.52 and 0.63 in midseason and late season, and the K _cp value was adjusted as 1.29 after soil wetting in rice field under drying–wetting cycle condition. The dual-crop coefficient curves based on locally calibrated K _cbCal and K _cpCor matched well with the measured crop coefficients and performed well in calculating rice evapotranspiration from paddy fields under drying–wetting cycle condition. So it can be concluded that the procedure of dual-crop coefficient method is feasible in rice ET _c estimation, and locally calibrated K _cb and K _cp can improve its performance remarkably.     

Response of organic carbon fractions and microbial community composition of soil aggregates to long-term fertilizations in an intensive greenhouse system

Journal of Soils and Sediments - Soil organic carbon (SOC) content and stability, which are regulated by microbial communities, vary depending on aggregate size. The objectives of this study were...     

外源添加剂对黄贮小麦秸秆产甲烷潜力及微生物群落的影响

为探讨添加不同外源添加剂对黄贮小麦秸秆产甲烷潜力和微生物群落结构的影响,该研究以小麦秸秆为黄贮原料,添加乙酸(3‰ACE组)和异型发酵乳酸菌复合系(3‰MI1组,6‰MI2组),调整含水率至50%,黄贮65 d后,以黄贮小麦秸秆为厌氧发酵原料,探究发酵过程中的指标性质和微生物群落结构。研究发现黄贮预处理后,发酵系统中的初始挥发性有机酸中的乳酸和乙酸增加、甲烷含量增加、可溶性化学需氧量(sCOD)增加。添加了外源添加剂的ACE组、MI1组、MI2组的累积甲烷产量较干黄小麦秸秆(WS组)提高了4.7%～10.6%,而未添加添加剂的CK组的累积甲烷产量较WS组降低了9%。发酵系统中的主要优势细菌为Bacteroidetes、Firmicutes和Proteobacteria,优势古菌为甲烷鬃菌属Methanosaeta,黄贮预处理改变了发酵系统微生物的群落结构,促进了小麦秸秆的甲烷转化,为木质纤维素的沼气转化提供参考。