马铃薯渣作培养代料的两种侧耳属食用菌菌糠中两种饲用酶活性的检测研究

本研究以培养代料含马铃薯渣的凤尾菇菌糠、小白侧耳菌糠为研究对象,检测其中植酸酶及β-葡聚糖酶的酶活。凤尾菇菌糠植酸酶酶活在pH2.5下为0.0822U/g,在pH5.2下为0.0786U/g。小白侧耳菌糠植酸酶酶活在pH2.5下为0.1052U/g,在pH5.2下为0.1027U/g。凤尾菇菌糠β-葡聚糖酶酶活0.0260U/g,侧耳β-葡聚糖酶酶活为0.0354U/g。结果表明,加入马铃薯渣做培养代料的凤尾菇、小白侧耳菌糠具有一定的饲用酶活性,用其作禽畜饲料加工时,可适量减少饲用酶的加入量,以节约成本。     

山羊多头蚴及其异位寄生病例的诊治

多头蚴病俗称脑包虫病,是带科多头属多头绦虫的幼虫寄生于牛、羊脑或脊髓内发生的疾病。多头蚴的成虫寄生于狗、狼等肉食动物的小肠内,孕卵节片随粪便排出体外,从孕卵节片内释放出大量虫卵,污染饲草、饲料或饮水,牛、羊等中间宿主吞食后,六钩蚴钻人肠黏膜的毛细血管,随着血液循环带到脑和脊髓等易寄生部位,以脑部寄生为多。     

蚕粪中提取叶绿素铜钠盐的稳定性研究

以蚕粪为原料,95%的乙醇为溶剂提取叶绿素,并通过化学法制得叶绿素的铜纳盐。采用721分光光度计对叶绿素铜钠盐进行定性、定量测定,确定该色素测定系统中的最大波长为414nm。通过测量吸光值研究了几种常用食品添加剂对叶绿素铜钠盐稳定性的影响,并对其耐光、耐热、耐酸碱及耐氧化性、耐还原性进行探讨。结果表明:叶绿素铜钠盐在70℃以下稳定性较好;光照对叶绿素铜钠盐稳定性的影响较大;还原剂(Na2S2O3)对叶绿素铜钠盐稳定性的影响较大,氧化剂在短时间内对其稳定性的影响不大,但放置的时间不宜超过30min;叶绿素铜钠盐在pH值为6以上的溶液中较为稳定,且有机酸及单糖对其稳定性无明显影响。     

浙江省缙云县农机服务专业合作社发展现状与对策

为发挥农机服务专业合作社在浙江省缙云县农业生产中的作用,归纳总结了缙云县农机服务专业合作社发展的现状和成效,并对该县当前农机服务专业合作社发展存在的问题进行了分析,在此基础上提出了加快发展缙云县农机服务专业合作社的相关措施建议。      

比利时牧羊犬

<正>我国的养犬爱好者,对比利时牧羊犬(Belgian Shepherd Dog)的认识多半是来自比利时马里努阿犬(Belgian Malinois)。因为自20世纪末期开始,我国逐年进口马里努阿犬,尤其公安、军队、武警、海关等行业,     

Selection and Evaluation of Indexes Commonly Used to Determine Contamination with T-2 Toxin in Pacific White Shrimp Litopenaeus vannamei by the Grey Relational Method

Abstract

The objectives of the present study were to evaluate the effects of different concentrations of the mycotoxin T-2 toxin in feed on muscle performance in the Pacific white shrimp Litopenaeus vannamei, evaluate indexes of physiological variables that indicate T-2 toxin contamination in the shrimp using the grey relational method, and determine the dose–response relationships between T-2 toxin and the indexes. Of the 6 physical, 7 biochemical, and 17 nutritional indexes examined, the values of the grey relational coefficients were highest for the hepatopancreas: body weight ratio (HBR), alanine aminotransferase (ALT) activity, and serine (SER) content (0.83, 0.68, and 0.82, respectively). Therefore, the HBR, ALT activity, and SER content were selected as appropriate indexes for contamination of Pacific white shrimp muscle with T-2 toxin. Based on their dose–response relationship curves, mean effective doses of 1.45, 1.69, and 1.33 mg of T-2 toxin/kg of feed were obtained for the HBR, ALT activity, and SER content, respectively. These results offer technical reference points for the evaluation and control of T-2 toxin in shrimp feed.

Received April 28, 2016 accepted April 9, 2017 Published online July 26, 2017     

断奶日龄对仔猪Et玫瑰花环形成率和T淋巴细胞转化率的影响

选取15窝杜洛克×长白×约克夏三元杂交初生仔猪 ,按14、21、28、35日龄断奶和不断奶随机分为5组(14E、21E、28E、35E、C) ,每组3窝 ,研究断奶及不同断奶日龄对仔猪外周血Et玫瑰花环形成率和T淋巴细胞转化率的影响。结果表明 :哺乳仔猪外周血Et玫瑰花环形成率、淋巴细胞转化率LTT(PHA、LPS刺激原)随日龄增长呈线性加大的趋势(P<0.05) ;断奶应激均使Et、LTT(PHA)、LTT(LPS)降低(P<0.05)。断奶越早 ,断奶后血液LTT(PHA/LPS)下降的幅度越大 ,恢复与哺乳猪相同水平的时间越长 ,其中14日龄断奶后1～3周 ,21、28日龄断奶后1周LTT(PHA)显著低于对照组(P<0.05) ;14日龄断奶后1～4周 ,21日龄断奶后1～3周 ,28日龄断奶后1～2周 ,35日龄断奶后1周LTT(LPS)显著低于对照组(P<0.05)。     

台湾海峡及其邻近海域渔业资源管理的探讨

应用Schacfcr和Fox剩余产量模式及其由此衍生的生物经济模式和Gulland最适产量Y_(0.1)模式,分别估算了台湾海峡及其邻近海域渔业资源的最大持续产量、最大持续捕捞力量,最大经济产量、最大经济捕捞力量、最佳经济效益,最适产量、最适捕捞力量,并对各模式计算的诸项经济指标进行比较,讨论了渔业管理方案,确定了近期适合国情、省情的管理目标。     

Genetic differences in oestrous signs and oestrogen metabolism‐related genes between Chinese Mi and European Landrace‐Large White pigs

Oestrous signs affect timely mating and reproductive efficiency in swine breeding herds. To study the genetic difference of oestrous signs between Chinese and European pigs, 100 Landrace‐Large White (LLW) cross gilts and 50 Chinese Mi gilts were assessed for oestrous signs and the concentrations of serum estradiol‐17β and progesterone were determined. The genotype of 39 single nucleotide polymorphisms (SNPs) in 11 oestrogen metabolism and function‐related genes was determined by Sequenom iPLEX platform. Compared with LLW gilts, Mi gilts had longer time of standing reflex (p < .001), higher scores of vulva reddening (p = .001) and greater serum estradiol‐17β concentration (p < .01). Gilts with greater serum estradiol‐17β concentrations also had greater (p < .05) scores for oestrous signs. Genetic polymorphisms of nine genes in oestrogen metabolism pathways had significant differences (p < .05) between LLW and Mi gilts. There were three and six haploblocks of SNPs in LLW and Mi, respectively. Compared with LLW, the distribution of haplotypes was more centralized in Mi pigs. Genetic polymorphisms of oestrogen metabolism‐related genes have considerable differences between Chinese Mi and European LLW pigs. Because of the important roles of oestrogen during the oestrus, some genes of oestrogen metabolism pathway could be considered as candidate genes for oestrous signs.     

闽南地区牛,羊消化道8种线虫幼虫的形态观察

取闽南地区牛、羊消化道常见的８种线虫幼虫，对它们进行了形态学观察及比较，以期提供用于生前诊断的参考资料，