生态营养饲料及其配制技术

随着经济的不断发展,人们生活质量的逐步提高,不仅要求食物富含营养、卫生安全,而且要求有良好生活环境。因此,当前世界上回归大自然的呼声越来越高,生产绿色食品和加强环境保护成为人们关注的热点。畜牧业是人类赖以生存和发展的基础产业,生产绿色畜产品和减少畜牧...     

芸薹属种子休眠特性的研究——Ⅲ.不同发芽促进剂及热震荡对油菜种子的破眠效果

本文研究了不同发芽促进剂及热震荡对芸薹属的不同种质的破眠效果,结果表明:(1)野生或自生种质的种子与栽培种质相比,均有较深的休眠性,其中尤以云南野生白菜(Brassiacampestris L.)及新疆毛芥(B.xinjiangensis Y.Z.Lan)的休眠性为强。(2)GA_2结合其他发芽促进剂与单独GA_2处理,对种子的萌发都有明显的促进作用,破眠程度(DBE)值在0.78—0.86之间,两者之间不存在显著差异,但是不同种质的DBE变化较大,以较深休眠的自生芥菜(B.juncea L.)效果最差。(3)热震荡对于解除芸薹属自生芥菜种子休眠的效果更差,劣于200ppm GA_3。     

磷对板栗结实性能及产量的影响

试验结果表明,磷对板栗(Castanea mollissima Blume)雌花的形成数量有重要影响,结果母枝含磷量与母枝抽生结果枝数量和着生雌花数量呈显著正相关。板栗结果母枝含磷量比雄花母枝和营养枝分别高5.19％和5.27％。树体磷素含量水平与板栗雌、雄花枝比例和结果枝结栗苞数量也存在密切的相关性。在不同施磷水平下,结果枝粗度与果枝结栗苞数均具有显著的回归关系,但b值差异大。本研究结果在鄂东大别山区进行了大面积施磷肥示范,生产实践证明,施磷肥对板栗具有一致的增产效益。     

不同断奶日龄仔猪消化道酸度和胃蛋白酶活性的动态变化

将12窝同日龄新生仔猪随机分成4组，每组3窝，每窝为一个重复组，分别采取17、21、28和35日龄断奶，在断奶后12h、1周、2周和3周从每个重复组中随机抽取1头屠宰，共测定了54头仔猪胃、十二指肠、空肠和回肠食糜pH值及胃食糜中胃蛋白酶的活性。结果发现断奶后12h胃食糜pH值均高于同龄哺乳仔猪，并且达该组整个试验期间的最高，随后逐渐降低。4组仔猪在断奶后胃pH值回复到3.5所需的时间分别为3、2、1和1周，这表明断奶日龄越早，胃中pH值回复到3.5所需要的时间越长。 除十二指肠外，空肠和回肠食糜pH值基本上不受日龄和断奶的影响。哺乳仔猪从18－36日龄胃蛋白酶相对活性（U/ml胃液）和比活（U/mg蛋白质）增加10倍以上。不同断奶日龄仔猪各组在断奶后12h胃蛋白酶的相对活性和比活均聚然升高，达到该日龄铒猪阶段正常值的2倍左右，特别是小日龄断奶仔猪为明显，随后在3周内逐渐降低，至50日龄时稳定在一个较低水平。     

绿脓假单胞菌致死海豚的病理变化

1997年～1998年青岛市海豚表演馆饲养的3只海豚小青、小红、小黑分别于1997年9月、1998年2月、5月因绿脓假单胞菌感染而引起发病. 　　……     

常见儿科病红细胞免疫粘附功能检测的临床意义

检测儿科常见疾病患者中的红细胞免疫粘附功能发现：（１）新生儿的红细胞免疫粘附功能尚未发育完善，低于正常儿童水平；（２）急性肾炎患儿的红细胞免疫粘附功能呈现继发性增强；（３）肾病综合征、风湿性疾病、大叶性肺炎、秋季腹泻及急性白血病等患儿的红细胞免疫粘附功能呈继发性降低；（４）红细胞免疫粘附功能的变化与疾病的发展及转归有关。提示在儿科疾病的诊断和治疗中观察患儿的红细胞免疫粘附功能有重要意义。     

Efficacy of different dosages of ivermectin injectable against the Hypoderma spp. in yaks

A chemotherapy trial was conducted to determine the lowest dosage of injectable preparation of ivermectin against Hypoderma spp. infestation in yaks in Tibetan areas in Tianzhu county, Gansu province, in northwest of China. One hundred and sixty yaks were randomly divided into four groups of 40 yaks for the trial. The first three groups were treated by subcutaneous injection in the neck with 0.1% ivermectin (respectively, 1, 5, 10 microg/kg body weight). The fourth group was not treated and considered as control group. All the experiments were performed in November 2000 and the animals were examined for the presence of warbles in the next March and May. The results indicated that there was no warbles found on the back of treated animal while third stage larvae were palpated on back of some of the yaks in control group. It is concluded that dosage of 1 microg/kg ivermectin injectable was sufficient to kill or stop development of larvae of Hypoderma spp. in naturally infected yaks if administrated in November.     

Single primer polymerase chain reaction fingerprinting for Pasteurella multocida isolates from laboratory rabbits

OBJECTIVE: To evaluate a rapid polymerase chain reaction (PCR) fingerprinting technique for discriminating among Pasteurella multocida isolates from laboratory rabbits. SAMPLE POPULATION: 33 P multocida isolates from rabbits with clinical pasteurellosis. PROCEDURE: PCR assays were conducted with 2 minisatellites (core sequence and modified core sequence of phage M13) and 2 microsatellites ([GTG]5 and [GACA]4). Each bacterium was assigned to a PCR type for each of the primers used. Boiled bacterial extracts and purified genomic DNA were compared by use of PCR assays for phage M13 and (GACA)4. Plasmids were isolated from each bacterium, and their influence on PCR fingerprint was determined, using boiled extracts as a DNA source. RESULTS: M13 core sequence and M13 modified core sequence yielded 5 and 8 PCR types, respectively. The microsatellites (GTG)5 and (GACA)4 yielded 4 and 9 PCR fingerprint types, respectively. Fingerprint patterns obtained by use of isolated DNA differed from those obtained by use of boiled extracts, although discrimination among P multocida isolates was similar. The presence or absence of plasmids did not affect PCR fingerprints. CONCLUSION: Single primer PCR fingerprinting with minisatellite and microsatellite primers is an efficient and reproducible method for the discrimination of P multocida isolates from rabbits and can be performed directly, using boiled bacterial extracts as a source of template, although more bands were obtained from pure genomic DNA.