酿酒葡萄成熟特性的研究

葡萄浆果成熟过程中,存在着多方面的变化。只有含糖量、含酸量的变化最有规律,最能代表葡萄品种的成熟特性。含糖量的变化与logistic曲线、含酸量的变化与对数曲线拟合性最好。不同品种成熟特性的差异,主要表现为含糖量上升速率与含酸量下降速率的不同。气象因素能明显影响葡萄的成熟特性,最终造成同一品种在不同地区成熟度的差异。     

东亚飞蝗蝗蝻抽样的研究

自五十年代以来,应用于东亚飞蝗蝗区估计蝗蝻种群密度的抽样方法,是样方为10平方尺的筒单随机抽样。本文是以抽样数、抽样消耗、相对精确度为标准,对三种抽样模型、三个抽样单位进行了比较。根据这些结果,我们提出了在当前蝗蝻密度较低的情况下,应采用样方为100平方尺的最适配额分层抽样。这种抽样方法在精确度方面可以高出现用的抽样方法2倍以上。而抽样时间消耗可相对节约一半以上。     

低温胁迫下解放钟枇杷贮藏特性研究

枇杷果实皮薄多汁，成熟期较集中且适逢高温季节，采后在常温下极易失水皱缩和衰老变质，不耐贮藏和长途运输，损耗率极高。解放钟枇杷是福建省的主栽品种，4月中下旬成熟，果实特大，单果重70-80g，很受国内外市场欢迎。但采后常温下果实极易腐烂变质，果实的远程贮运受阻，外销更困难。国内外对枇杷采后的呼吸变化及其生理作用，已经有相关报道，但对解放钟枇杷果实采后呼吸及其他生理变化缺乏研究。为此，开展了本试验研究，以期为解放钟枇杷果实的贮藏保鲜和远程运输提供理论依据。     

动物骨粉的应用研究

动物骨头是肉类加工的副产品，由于富含蛋白质，钙、磷等微量元素以及多种维生素等营养成分，具有较高的营养价值，其对动物生产性能的促进作用早已被人们所认知，将其加工为骨粉后用于畜牧养殖已在生产实践中大量利用。了解骨粉的营养特性，掌握骨粉的加工及应用方法，将有助于充分发挥骨粉的营养功效，提高骨粉的利用价值。     

The mechanisms of pathophysiological vasodilation or vascular hyporesponse

In some pathophysiological processes such as severe shock, the patients and animal experimental models fail to respond to pressor medicine. Vascular hyporesponse or vasodilation is one of the key problems in treatment of these patents. Although considerable progress had been made in recent years, the precise mechanisms of vascular contractile hyporeactivity have not been completely elucidated. A number of studies indicate that vascular hyporesponse or vasodilation correlate with many factors. In this review, the possible mechanisms about vascular hyporesponse or vasodilation during shock and other pathphysiolgoical processes were discussed.     

Biological characteristics of long passaging rat mesenchymal stem cells

AIM:To investigate multi-potential of rat bone marrow mesenchymal stem cells (rBMMSC) and mutation inclination, the rBMMSC were long passaged in vitro. METHODS:Cellular cycles of different passages were assayed by FACSan flow cytometry and karyotypes of passage 6, passage 25 and passage 45 were compared by G-binding analysis. RESULTS:The early passages and long-term passages all showed strong proliferation; passage 6, passage 25 and passage 45 all showed normal karyotype. CONCLUSION:Long-term culture and passage of rBMMSC still remains strong proliferation. With this capability, the mutation inclination is not enhanced.     

不同栽培方式对生菜产量及品质的影响

采用土壤栽培、有机生态型无土栽培、无机耗能型无土栽培三种栽培方式，进行生菜三次采收四层立体栽培试验。结果表明：采用有机生态型无土栽培进行生菜分次采收立体栽培，可有效降低产品中硝酸盐含量，提高根冠比和相对生长速率，变一次性收获为三次采收，可使每盘产量提高30％以上，变平面栽培为4层立体栽培，使温室复种指数提高4倍。     

变叶海棠种群多样性的形成与分化研究

 采用杂种指数和形象化散点图法, 探明了变叶海棠以兼性无融合生殖方式与陇东海棠和花叶海棠产生渗入杂交变异, 又以无融合生殖方式保持新产生变异的遗传, 经过数千万年的世代繁衍,形成现今这样极其复杂多样的渗入杂交群体, 该群体为3 个种的杂交复合体。由于杂种的全部或部分能育子代中发生的基因重组和增加基因的突变, 丰富了物种的基因库, 从而深入的揭示了变叶海棠具有多种高抗性的遗传机理。在变叶海棠居群内保护或栽植适量的陇东海棠和花叶海棠, 对于保持和促进变叶海棠多样性的形成与分化具有重要意义。     

入世后我国甜樱桃面临的机遇与挑战及发展对策

甜樱桃作为一种早熟、优质、保健果品,而且种植效益较高,在国内国际都正处于发展时期。根据世界甜樱桃生产和贸易情况,指出了我国在甜樱桃科学技术与生产上与世界先进甜樱桃生产国的差距,分析了我国加入WTO之后,我国甜樱桃面临的机遇和挑战,并且机遇大于挑战。如果有适当的科技投入,我国甜樱桃产业将可克服现在科技滞后的状态,发挥我国劳动力低廉和国土辽阔的优势,迅速扩大其生产总量,提高品质,扩大出口,增加农民的收入,2015年可望形成32.4～43.2亿元的社会总产值。     

Preparation of gfp-bcl-XL-contained recombinant adenovirus vector by the homologous recombination in bacteria

AIM: To prepare gfp-bcl-X_L-contained recombinant adenovirus(rAd-gfp-bcl-X_L).METHODS: Bcl-X_L gene was amplified from pEGFP-C₃-bcl-X_L, subcloned into shuttle plasmid and formed transfer plasmid of pAdTrack-CMV-bcl-X_L. Then pAdTrack-CMV-bcl-X_L was linealinzed with PmeI and co-transformed into BJ5183 bacteria with adenovirus genomic plasmid of pAdEasy-1. The identified recombinant adenovirus plasmid was digested with PacI and transfected into 293 cells to package recombinant adenovirus particles. The target gene was detected by PCR.RESULTS: There were about 35% positive recombinant bacterial clones after the co-transformation of pAdTrack-CMV-bcl-X_L and pAdEasy-1 into BJ5183. Recombinant adenovirus particle were produced and further amplified after the transfection of pAdEasy-1-gfp-bcl-X_L into 293 cells. PCR test indicated that the recombinant Ad contained bcl-X_L gene. The titer of the purified rAd-gfp-bcl-X_L was 6.5×10¹² PFU/L. CONCLUSIONS: The homologous recombination in bacteria is a convenient and high efficient method to prepare rAd-gfp-bcl-X_L. This affords a good gene transfer vector for the gene therapy in human’s diseases.