猪LPL基因PCR-RFLP遗传变异的研究

利用PCR-RFLP技术,对大白猪、长白猪、杜洛克、荣昌猪、内江猪、八眉猪、汉中白猪、汉江黑猪8个家猪品种和1个野猪种共368头猪LPL基因的第6和第8内含子进行遗传变异研究。结果表明:(1)在第6内含子的Sma -RFLP位点上,各猪种均存在多态性,长白、杜洛克、内江猪和八眉猪以AA和AB基因型为主,大白、荣昌猪、汉中白猪、汉江黑猪及野猪以AB和BB基因型为主;在Pvu -RFLP位点上,国外猪种均为DD纯合子,而国内猪种和野猪在此位点上均存在变异;(2)在第8内含子的Hind -RFLP位点上,国外猪种和荣昌猪表现出多态性,而国内其他猪种和野猪均为FF纯合子;在EcoR -RFLP位点上,各猪种均表现多态性,且以GH杂合子为主,没有表现出差异。     

植物几丁质酶及其应用研究进展

几丁质酶是高等植物普遍存在的一种重要的病程相关蛋白,近年来被广泛应用于植物抗病基因工程的研究.本文概述了国内外有关植物几丁质酶的研究情况,介绍了几丁质酶的特性、结构、分类、细胞学定位和功能及几丁质酶基因的克隆与其在植物基因工程中的应用.     

Analysis on Dynamic Heterosis for Allelopathy in Rice Under Different Environment Conditions

In this study, 5 parental rice varieties with different allelopathic potentials were employed indiallel cross [P(P+ 1)/2] to get a set of genetic materials including parental lines and two generations of F1 s.The dynamic heterosis for allelopathy in rice under different environmental conditions, was analyzed by usingadditive-dominant developmental genetic model. The results indicated that heterosis in both F1 and F2 showedinhibitory effects on shoot and root length of receiver plant (Lactuca sativa L. ). Heterosis over mid-parentbased on population mean(HMP) in F2 was lower than that in differental environmental conditions, showing1/2 HMP in F1. The heterosis in rice allelopathy was much higher under the field environmental conditionswith lower temperature and weaker sunlight than that under favorable environment, implying that the allel-opthic potential could be increased by stress environment. This finding interpreted the genetic reason thatplant could produce more allelochemicals under unfavorable environment.     

菌料搅拌机混合质量的试验研究

菌料中所包含的各种成分的混合均匀程度直接影响食用菌的质量和产量.在分析影响菌料搅拌机混合质量诸因素的基础上,提出了影响工作性能的主要参数是搅拌装置的转速、混合时间和填料量,并对其进行了正交试验分析,以此建立了上述3个参数的最优组合转速为23r@min-1,混合时间为3min,填料量为100kg.该菌料搅拌机的混合均匀度可达96%以上,保证了菌料的混合质量.     

福建竹类害虫发生特点、成因及综合防治探讨

在长期调查研究福建省竹类害虫的基础上 ,深入分析了竹类害虫发生的基本特点 ,主要表现为种类多、分布广、频率高、危害严重且新害虫不断出现 .同时从不同角度分析了害虫发生的主客观原因 ,探讨了害虫发生与环境条件、竹林管理水平、某些非生物因素以及检疫措施等因素之间的关系 .最后对福建省多年来所实施的竹类害虫综合防治进行小结     

20种树木提取液对蔬菜害虫胃毒作用的研究

采集20种树木的叶、花和果实等材料，预处理后分步用石油醚、乙醇和水为溶剂，依次浸溃制备成树木提取液。分批对蔬菜害虫中的菜青虫和小菜蛾进行胃毒作用测定：(1)初选出乌桕、樟树等lO种树木对害虫毒效超过l0％的14种提取液；(2)试验结果显示，乙醇提取液对蔬菜害虫胃毒作用比石油醚提取液的效果好；(3)建议提高供试药液的浓度；(4)试验为进一步复筛试验提供了借鉴和准备工作。     

辣(甜)椒抗TMV、CMV、疫病及炭疽病材料筛选

病毒病、疫病、炭疽病由于发生普遍、危害严重，一直是辣(甜)椒生产中最主要的三大病害。据报道世界上危害辣(甜)椒的病毒有45种，在我国有8种病毒侵染辣椒，其中烟草花叶病毒TMV、黄瓜花叶病毒CMV是主导毒源，常造成减产20％～70％。辣椒疫病是由Phytophthora capsici引起的一种毁灭性的病害，我国的吉林、辽宁、青海、河北、北京、云南、甘肃、陕西、上海、浙江、湖南、江苏、     

浅谈农业数字图书馆建设

结合本馆实际，探讨了网络环境下农业图书馆实现数字化的必然性和可行性，提出了农业数字图书馆建设的有效措施。     

DNA分子的电镜制样及观察

摸索了ＤＮＡ分子的水展膜电镜制样方法与技术，在无旋转投影设备的条件下，直接用透射电镜观察到了ＤＮＡ分子的线型结构。     

Effects of Long-term Located Fertilization on the Physico-chemical Property of Soil Humus

A systematic study concerning the effects of a long-term stationary fertilization on content and property of soil humus in fluvo-aquic soil sampled from Malan Farm, Xinji City, Hebei, and arid red soil and paddy red soil sampled from the Institute of Red Soil, Jinxian County, Jiangxi was conducted. The results showed that long-term fertilization had effects not only on the content and composition of soil humus, but also on the physico-chemical property of humus. With applying organic manure or combined application of organic manure and chemical fertilizer, E4 and E6 values of humic acid decreased in fluvo-aquic soil and arid red soil,but increased in paddy red soil. In paddy red soil, E4 and E6 values of humic acid increased also with a single application of chemical fertilizer, but E4 and E6 values had less change of humic acid in fluvo-aquic soil and arid red soil. The effects on the visible spectroscopic property of fulvic acid were different from that of humic acid. Long-term application of organic manure or combined application of organic manure and chemical fertilizer could increase E4 and E6 values of fulvic acid in three types of soil. Single application of chemical fertilizer had less effect on the E4 and E6. Long-term fertilization could also influence the ultraviolet spectroscopic property of humus. With a single application of organic manure or combined application of organic manure and chemical fertilizer, the ultraviolet absorbance of humic acid and fulvic acid increased in the three types of soil.But this effect was obvious only in short wave length, and the effect could decrease if the wave length increased. With a single application of chemical fertilizer the ultraviolet absorbance of fulvic acid could increase, but it of humic acid increased only in fluvo-aquic soil. Long-term application of organic manure or combined application of organic manure and chemical fertilizer could increase the content of total acidic groups, carboxy groups and phenolic hydroxy groups of humic acid and fuvic acid in the three types of soil.Single application of chemical fertilizer had less effect on the content of total acidic groups, carboxy groups and phenolic hydroxy groups of humic acid and fuvic acid in the three types of soil.