灰树花不同配方栽培研究

对灰树花七种栽培配方进行了研究，其结果表明：木屑和棉籽壳作为栽培的主要原料，若等比例混合。生物学效率最高；麦麸与玉米粉搭配使用，可以提高灰树花的产量及生物学效率；平菇废料是栽培灰树花较好的原料，并具有很好的经济效益、社会效益和生态效益；并筛选出灰树花高产的三个配方。     

不同温度下转换寄主对桃蚜生态学特征的影响

在5种温度、4组寄主与蚜源组合处理条件下研究了温度对转换寄主后的桃蚜的影响。同一寄主间移植的桃蚜在有效积温、发育起点温度、生殖率、死亡率、寿命以及抗逆性上与不同寄主间移植的桃蚜有明显的差异;寄主对桃蚜生物学、生态学特征有明显的影响,表明长期生活在一种寄主上的桃蚜获得了一定的专化性。若将其移植到另一种寄主上,则会表现出种种不适反应,但这种反应还未进化至型或寄主生殖隔离。     

国产克洛素隆尿药排泄数据分析

应用Kromasil-C18色谱柱(250 mm×4.6 mm,5 μm),WatersTM480型可调波长紫外检测器,0.01M磷酸钾(pH=7):乙腈(3:1)为流动相,检测波长265 nm,含量测定采用标准曲线法,建立了RP-HPLC法检测绵羊尿中克洛素隆含量的方法.方法有效性评价结果表明,尿药含量在0.01～5.0μg/ml及5.0～30.0μg/ml范围呈良好线形关系(r=0.9993、0.9995),方法平均回收率99.32%,日内、日间变异系数分别为3.91%、6.28%.尿药最低检测限0.005μg/ml.试验绵羊以7 mg/kg单剂量经静脉、肌肉及口服三种途径给药后,尿中药物浓度分析表明,克洛素隆经体内处理后主要经肾脏排泄,给药96 h内,静注经尿排泄原药为81.76%,肌注为64.03%,口服为48.50%.     

富铁酵母对家兔红细胞免疫功能的影响

为探讨富铁酵母对家兔红细胞免疫功能的影响,将32只家免随机分成富铁酵母高、低剂量组,FeSO4组及空白对照组,分别灌服富铁酵母(Fe元素8 mg/kg、4 mg/kg)、FeSO4(Fe元素4 mg/kg)及空白生理盐水;连续5 d后,检测红细胞C3g受体花环率和复合物(IC)花环率.结果:富铁酵母较FeSO4及空白组能显著提高红细胞C3h受体花环和红细胞复合物(IC)花环的百分率,但高、低剂量间无显著性差异.结论:富铁酵母能显著提高红细胞免疫功能.     

玉米螟赤眼蜂对亚洲玉米螟种群的控制作用

为了解决深圳特区甜玉米产区日趋严重的农药污染问题,笔者在深圳龙岗生态示范农场甜玉米地研究了玉米螟赤眼蜂Trichogramma ostriniae的应用技术,分析了玉米螟赤眼蜂释放量、释放次数对亚洲玉米螟Ostrinia furnacalis Guen6e控制作用的关系,提出了最件释放方法.     

Regulation of MMP-2 and TIMP-3 expression by uPA signal transduction system in human bone giant cell tumor

AIM: To study effects of urokinase-type plasminogen activator (uPA) signal transduction on expression of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of matrix metalloproteinase-3 (TIMP-3) in giant cell tumor of bone (GCT). METHODS: Expression of uPAR, MMP-2 and TIMP-3 in GCT tissue was detected by immunohistochemistry. Phosphorylation level of mitogen-activated protein kinase (p44) in uPA/uPAR signal pathway in cultured GCT cells was detected by immunoprecipitation. The expression of MMP-2 and TIMP-3 in cultured cells after treatment with uPA-ATF or anti-uPAR antibody was also detected by Western blotting. RESULTS: 1) Urokinase-type plasminogen activator receptor (uPAR) was positive on the cell membrane and in cytoplasm of some mononuclear stromal cells (MSCs) and multinucleated giant cells (MGCs); 2) MMP-2 was positive in the cytoplasm and on the cell membrane of almost all of MSCs and some of MGCs. The polar distribution of MMP-2 in the cytoplasm of MGCs was especially obvious; 3) The expression of TIMP-3 of some MSCs and MGCs in GCT was much lower than MMP-2. The positive signal also showed a prominent polarity; 4) After treatment with uPA-ATF, the phosphorylation level of p44 in GCT cultured cells was much higher than the control. Addition of anti-uPAR antibody in the cells remarkably down-regulated the phosphorylation level of p44 as compared with the control group, suggesting that uPA-ATF participates cell signal transduction and this reaction can be inhibited by anti-uPAR antibody; 5) uPA-ATF cell signal pathway up-regulated expression of MMP-2 and TIMP-3, while anti-uPAR antibody down-regulated the expression of MMP-2 and TIMP-3. CONCLUSION: These results demonstrate for the first time that uPA-ATF directly regulates the expression of MMP-2 and TIMP-3 by signal transduction pathway, and the over-expression of MMP-2 and TIMP-3 may play an important role in local osteolysis of GCT.     

金龟子绿僵菌固态培养生物变量优化研究

金龟子绿僵菌属于真菌类生物杀虫剂,本文研究了接种量、种龄、菌种代数等生物因素对金龟子绿缰菌生长及产孢量的影响,并对液—固两步发酵工艺进行了研究。经过优化,得到金龟子绿僵菌产孢的最佳生物参数是:接种量为2.5g/100g、种龄为5-6天;采用固体二代种,培养6天,孢子产量可达1.531×10~(10)孢子/g干培养基。     

几种绿色饲料添加剂在肉鸡生产上的应用效果

选1日龄AA肉鸡390只,分成13组,分别添加不同种类和不同种类组合的绿色饲料添加剂。试验结果表明,益生素能显著(p<0.05)提高肉鸡的生产性能,改善肉品质。     

日粮维生素E水平对后备种母鸡生长发育的影响

研究日粮维生素E对后备种母鸡体形、肌肉和生殖器官生长发育的影响。发现日粮维生素E水平对20周龄后备种母鸡的体重、体重均匀度、胸骨长度、胫长度、屠宰率、半净膛率、全净膛率、胸肌率、腿肌率、翅重率、腹脂率、冠高度和卵巢重量没有显著影响(P>0 05 ), 但高剂量维生素E日粮显著增加输卵管的长度和重量(P<0 05)。结果表明日粮高维生素E水平(180mg/kg) 可加速20周龄后备种母鸡输卵管的生长发育。     

复方中草药"毒菌杀"对禽大肠杆菌及鸡白痢沙门氏菌的体外抑菌试验

观察了复方中草药“毒菌杀”的安全性及其对禽大肠杆菌及鸡白痢沙门氏菌的体外抑菌情况。结果发现“毒菌杀”安全性高，组成“毒菌杀”的各单味中药及其合剂对大肠杆菌的最低抑菌浓度MIC(g/mL)分别为板蓝根0．05、穿心莲0．05、黄芪0．025、黄柏0．05、柴胡0．05、生地0．05、甘草0．05、当归0．025，“毒菌杀”方剂为0．05；对沙门氏菌的MIC分别为板蓝根0．05、穿心莲0．025、黄芪0．025、黄柏0．05、柴胡0．025、生地0．05、甘草0．025、当归0．05，“毒菌杀”方剂为0．025；而牛胆汁对这两种致病菌的最低抑菌浓度分别为2％和1％。说明“毒菌杀”方剂对大肠杆菌、沙门氏菌具有明显的抑制作用。