晚熟柑橘新品种‘华晚无籽沙糖橘’

 采用芽变选种的方法,从‘无籽沙糖橘’选育出晚熟柑橘新品种--‘华晚无籽沙糖橘’。果略小,单果质量45.0 g 左右,单果平均种子数2.0 粒以内,达到柑橘无籽的标准。果实扁圆形,皮橙黄色,薄而脆;果肉橙红色,味清甜,可溶性固形物含量14.43%,肉质爽脆化渣,品质优。早结实,丰产稳性好,发梢能力强,3 月中下旬开花,1 月下旬至2 月上旬果实成熟。     

丁二酸浸种对玉米幼苗生理代谢及产量的影响

采用盆栽和大田试验相结合,研究了不同浓度的丁二酸浸种对玉米幼苗生理代谢及产量的影响。结果表明,适宜浓度的丁二酸浸种能够促进玉米幼苗的生长,增加生物量,改善根部性状,提高幼苗叶片叶绿素和可溶性蛋白含量,增强硝酸还原酶活性;用200～400 mg/L浓度的丁二酸浸种可显著增加玉米产量,增产率为12.20%～12.50%。     

NAC对早期断奶仔猪抗氧化能力和免疫能力的影响

试验旨在研究日粮添加N-乙酰半胱氨酸(NAC)对早期断奶仔猪的氧化还原状况和免疫指标的影响。试验选取10窝仔猪,随机分为2组,每组5窝,对照组饲喂基础日粮,试验组从7日龄开始,至25日龄时结束,在基础日粮中添加500mg/kg NAC。仔猪21日龄断奶。25日龄时,每窝选取1头(每组共5头),采集血样和脾脏样。结果表明,日粮添加NAC组显著提高了血清谷胱甘肽过氧化酶(GSH-Px)和超氧化物歧化酶(SOD)水平(P0.05),降低了丙二醛(MDA)和过氧化氢(H2O2)(P0.05)的含量,同时增加了血清中免疫球蛋白IgM的含量(P0.05),而对仔猪血清炎性因子水平无显著影响。NAC处理能显著提高脾脏中IL-2,IL-6及I-κBαmRNA的相对表达水平(P0.05)。上述结果表明,日粮中添加500mg/kg NAC显著提高了断奶仔猪的抗氧化能力和免疫力,缓解断奶应激。     

椭圆锥临界截平面的分析与研究

通过分析和数学推导,建立了临界截平面的数学方程,确立了相应的平面作图方法,并对其应用进行了详细叙述。     

宝天曼自然保护区杂木林群落的植物特征分析

宝天曼自然保护区约有高等植物114科、334属、667种及变种，分别占宝天曼自然保护区植物科的51．12％，属的34．33％，种的24．60％；占河南等植物科的5．47％，属的25％和种的14．02％，组成本区杂木林植物的优势科有蔷薇科、菊科、禾本科、豆科等4科，合计199种，占本区被子植物总种数的32．4％，杂木林植物以温带成分为主，兼有一定热带分布类群，它体现了本区植物区系的过渡性、边缘性、多样性和独特性，显示出多方交汇和一定的古老性。本区杂木林群落垂直分布比较明显。     

Genetic and Association Mapping Study of English Grain Aphid Resistance and Tolerance in Bread Wheat Germplasm

English grain aphid （EGA） is a destructive insect pest of wheat. To identify the loci associated with EGA resistance and tolerance, 70 bread wheat accessions mainly from central Asia were evaluated for EGA resistance and tolerance traits at two locations, and genotyped with 51 SSR markers. Totally, three accessions showed high or moderate levels resistance and 17 genotypes displayed highly or moderately tolerate to EGA. Genetic diversity of these lines was investigated also. After 97 SSR loci which evenly covered all wheat chromosomes were scanned for association, four SSR loci were significantly associated with EGA resistance and four with EGA tolerance. After association analysis was conducted with dynamic aphid densities, we found four loci Xgwm192b, Xgwm391, Xbarc98, and Xgwm613b were detected continuously at different growing stages of wheat. In addition, the loci of EGA resistance/tolerance and Russian wheat aphid resistance were compared. The results generated in this study would be helpful for utilization of the EGA resistance/tolerance germplasm, and for development of mapping populations in EGA resistance breeding programs.     

花生根际解磷真菌的分离及其解磷能力研究

[目的]分离花生根际解磷真菌及测定其解磷能力。[方法]利用土壤稀释平板法,从花生根际土壤中分离筛选出解磷真菌PFK-2,接种于以Ca3(PO4)2为唯一磷源的液体培养基,振荡培养24、48、72、96、120、144、168 h时测定培养液的pH值、速效磷含量、菌丝混合物重。[结果]随着培养时间的增加,培养液pH值先降低后升高,菌株的溶磷量先增加后降低,菌丝混合物重量增加,当培养时间为120 h时,培养液pH值最低,速效磷含量最大。培养液pH值与菌丝重、菌株的溶磷量均呈极显著负相关(P<0.01)。[结论]在振荡培养120 h时,溶磷真菌PFK-2的解磷效果最佳。     

涪陵水牛mtDNA D-loop区遗传多样性研究

利用测序技术和生物信息学分析技术,对涪陵水牛27个个体的线粒体DNA D-loop 915 bp全序列的遗传多样性及系统进化进行分析,检测到16种单倍型,其核苷酸多态位点48个,约占所测核苷酸总长的5.24%,其中有43个转换,5个颠换。涪陵水牛mtDNA D-loop区核苷酸多样度(π值)为0.0196±0.0020,单倍型多样度(H)为0.9060±0.0460,表明涪陵水牛mtDNA遗传多样性丰富。根据单倍型构建了涪陵水牛的NJ分子系统树,发现存在沼泽型水牛支系A和B,表明涪陵水牛具有2个母系起源。     

鸡胸腺重和脾脏重性状的全基因组关联

【目的】全基因组关联分析（genome-wide association study,GWAS）是复杂性状和疾病相关基因定位的新策略。【方法】试验利用鸡60K SNP芯片对来自50个公鸡家系的728只北京油鸡进行SNP分型检测,采用全基因组关联分析方法对影响100日龄胸腺重和脾脏重的基因进行定位研究。【结果】结果发现24个达5%全基因组水平显著的位点,与100日龄胸腺重和脾脏重显著相关,并在这些位点附近发现Janus kinase 1（JAK1）、 zinc finger DHHC-type containing 8（ZDHHC8）、vav 3 guanine nucleotide exchange factor（VAV3）、SATB homeobox 1（SATB1）等候选基因;84个与这两个性状潜在关联同时达到5%染色体水平显著的位点。【结论】利用GWAS分析策略筛选和鉴定的重要突变位点及候选基因,将为揭示鸡免疫器官发育的分子调控机制和抗病育种分子标记辅助选择提供必要的分子基础。     

Sampling Investigation on Inhatitant Domestic Tap Water Consumption Situation and It’s Characteristics Analysis

Economics.Through sampling investigation and analysis on inhabitant domestic tap water consumption situation,it is given that economic factors are the main factors influence inhatitant's water consumption.The functional relations among inhabitant's income and water consumption,water price and consumption are given. The tap water's income elasticity of demand and price elasticity of demand is analyzed, along with the fluctuating range month by month of inhabitant's water consumption.Finally ,this paper offers the reference data for planning the ability of water supply and making policies of ladder water prices.