秋种马铃薯高产栽培技术

经过多年多点示范,总结出高山秋种马铃薯高产栽培技术。即秋种马铃薯以选择排灌水方便的沙壤土或黄壤土为宜,忌与茄科作物连作;8月上中旬,采用单垄双行双三角形播种,密度5.25万~6.00万株/hm2;合理施肥,注意防治病虫害。     

药剂拌种对小麦根部病虫害防效及产量的影响

药剂拌种是防治小麦根部病虫害安全有效且操作简单的方法。为了给冀中北地区小麦根部病虫害防控提供依据,2018年秋季在廊坊节水高效综合试验站小麦(中麦1062)播种前利用生产上近年使用的拌种剂酷拉斯、奥拜瑞、亮势进行拌种,以不包衣种子为对照(CK),研究了不同拌种剂处理对小麦根腐病、茎基腐病、纹枯病和孢囊线虫防效以及主要农艺性状和产量的影响。结果表明:在统一播种、统一管理的情况下,不同药剂拌种的小麦生育期进程、主要生育期农艺性状、产量及其构成因素差异及其与CK差异均不显著,但酷拉斯拌种对小麦根腐病、茎基腐和纹枯病防治效果均最好,亮势拌种对小麦纹枯病和孢囊线虫防治效果最好。     

微信公共领域里的失范行为及其法治规范建设

从哈贝马斯公共领域理论出发，提出了微信群与微信公众号已经初步形成了公共领域这一观点，并主要介绍了围绕微信公共领域的社交功能、自媒体传播功能和商业贸易平台功能而产生的失范行为。依法规范微信公共领域，可以从完善依法管理微信公共领域、依法运营微信公共领域、依法使用微信公共领域三个有机联系层面的法治规范展开。     

Cloning and Sequence Analysis of PTTG1 Gene in Luchuan Pig

This experiment was aimed to clone PTTG1 gene CDS sequence of Luchuan pig,and was analyzed by bioinformatics methods.A pair of special primers was designed according to predicted sequence of porcine PTTG1 in GenBank.The coding sequence of PTTG1 in Luchuan pig was amplified by RT-PCR,its gene sequence characteristics and protein structure was systemically analyzed by bioinformatics techniques.The results showed that the cloned PTTG1 fragment included a 609 bp CDS (coding 202 amino acids).The sequence multi-aligned results showed that Luchuan pig shared 90.15%,87.85%,87.52%,87.03%,76.03%,74.38%,55.74% and 44.48% of similar nucleotide sequence with that of Bos,Pan troglodytes,Homos,Macaca,Rattus,Mus,Gallus and Danio retio,respectively.The protein structure analysis results showed that the protein attributed hydrophilic protein without signal peptide,localized in cell cytoplasm and had 16 phosphorylation sites.The phylogentic tree of amino acid indicated that PTTG1 was highly conserved in the process of evolution of different species.The cloning and analysis of PTTG1 gene provided an important foundation for further study biological function of porcine PTTG1 during early embryonic development.     

中国沙棘良种选育及遗传改良研究进展与展望

中国沙棘良种选育及遗传改良研究虽起步较晚但进展迅速,在优良品种选育、优异种质引种及遗传改良等方面的研究中取得了重大进展.为给中国沙棘资源的可持续利用提供理论依据和科技支撑,在汲取国内相关研究成果及查阅有关学术论文的基础上,综述了中国沙棘优良类型选择、优良个体(品种)选育、引种驯化、杂交育种及遗传改良等领域的研究进展,指...     

Pathogen Analysis of Goat's Respiratory Diseases

To investigate the pathogens of goat's respiratory disease from a goat farm in Guangxi province, the epidemiological investigation, clinic observation, pathological examination, bacterium isolation and identification, biochemical test, PCR and animal experiment test were conducted.The preventive and control measures were taken on the basis of pathogen epidemiological characteristics and drug sensitive test results.The results showed that Mycoplasma, influenza virus and parasite were negative by isolation and culture or PCR, however, two strains of gram-negative bacteria named MS1 and MS2 were isolated from lung.MS1 was identified as Serratia marcescens by biochemical test and 16S rRNA sequencing, which shared 99% nucleotide homology with other Serratia marcescens in GenBank.And MS2 was identified as E.coil by the same methods that shared 99% nucleotide homology with other E.coli in GenBank.Animal experiment showed that two strains could cause death in mice.The drug sensitive tests showed two strains were highly sensitive to spectinomycin, amikacin, kanamicin and neomycin.Kanamycin and dexamethasone were used to treat the sick goats, and achieved good results.     

Preparation of Atherosclerosis Model Using Guangxi Bama Mini-pig

The atherosclerosis (AS) model was prepared in Guangxi Bama mini-pig,and the atherosclerosis index (AI) was preliminarily identified in occurrence of AS to provide the basis for the preparation of related models.20 Guangxi Bama mini-pigs were randomly divided into control group and experimental group which were fed with high fat and cholesterol diet to prepare the AS model.Blood biochemical indexes were detected in the process of model preparation and the correlation between AI and the results of vascular slices was analyzed to initially draw up AI of AS.The results of vascular slices showed that the incidence rates of AS of Guangxi Bama mini-pigs in experimental group and control group were 20% and 0,respectively.The association analysis between the results of vascular slices and AI in the pathogenic Guangxi Bama mini-pigs preliminarily suggested that AI was above 3.8 and lasted for more than 3 months during the attack.     

不同培养基成分对薄荷试管苗生根的影响

[目的]筛选薄荷试管苗生根的最佳培养基组合。[方法]以薄荷试管苗茎段为试验材料,探讨不同培养基成分、凝固剂种类及添加量对其生根的影响。[结果]在薄荷试管苗培养过程中,使用琼脂为凝固剂,且添加量为7 g/L的1/2MS培养基时,其生根时间最短,生根数和生根长度较高。[结论]为薄荷试管苗的大规模、优质、高效生产提供理论依据。     

茂兰喀斯特森林溪流蜻蜓目昆虫资源调查初报

通过调查和鉴定,从茂兰喀斯特森林溪流采集到蜻蜓63种,隶属11科,其中,蜻科25种,春蜓科4种,蟌科7种,蜓科6种,扇蟌科5种,大蜻科2种,色蟌科3种,溪蟌科4种,丽蟌科2种,隼蟌科4种,山蟌科1种;跳长尾蜓、黑尾灰蜻、黑丽翅蜻、红痣绿色蟌、大溪蟌、点斑隼蟌、白腹小蟌属于贵州新记录种。