全文获取类型
收费全文 | 5204篇 |
免费 | 208篇 |
国内免费 | 8篇 |
专业分类
林业 | 309篇 |
农学 | 159篇 |
基础科学 | 44篇 |
782篇 | |
综合类 | 829篇 |
农作物 | 243篇 |
水产渔业 | 436篇 |
畜牧兽医 | 2049篇 |
园艺 | 152篇 |
植物保护 | 417篇 |
出版年
2023年 | 33篇 |
2022年 | 50篇 |
2021年 | 100篇 |
2020年 | 117篇 |
2019年 | 126篇 |
2018年 | 87篇 |
2017年 | 101篇 |
2016年 | 97篇 |
2015年 | 105篇 |
2014年 | 117篇 |
2013年 | 244篇 |
2012年 | 283篇 |
2011年 | 348篇 |
2010年 | 192篇 |
2009年 | 153篇 |
2008年 | 342篇 |
2007年 | 291篇 |
2006年 | 296篇 |
2005年 | 300篇 |
2004年 | 267篇 |
2003年 | 272篇 |
2002年 | 263篇 |
2001年 | 57篇 |
2000年 | 65篇 |
1999年 | 69篇 |
1998年 | 51篇 |
1997年 | 44篇 |
1996年 | 42篇 |
1995年 | 38篇 |
1994年 | 46篇 |
1993年 | 54篇 |
1992年 | 45篇 |
1991年 | 34篇 |
1990年 | 41篇 |
1989年 | 35篇 |
1988年 | 32篇 |
1987年 | 37篇 |
1986年 | 28篇 |
1985年 | 35篇 |
1984年 | 40篇 |
1983年 | 36篇 |
1982年 | 20篇 |
1981年 | 33篇 |
1980年 | 23篇 |
1979年 | 24篇 |
1978年 | 22篇 |
1977年 | 18篇 |
1975年 | 18篇 |
1974年 | 23篇 |
1973年 | 24篇 |
排序方式: 共有5420条查询结果,搜索用时 15 毫秒
21.
Katherine Smallwood Jean‐Benoit Tanis Iain A. Grant Laura Blackwood David R. Killick Isabel Amores‐Fuster James Elliott Aran Mas Aaron Harper Mary Marrington Riccardo Finotello 《Veterinary and comparative oncology》2019,17(2):165-173
The DMAC protocol (dexamethasone, melphalan, actinomycin‐D, cytarabine) has been evaluated in American studies for the treatment of relapsed canine lymphoma, comparing similarly to other rescue protocols. The aim of this study was to evaluate efficacy and toxicity of DMAC, in a larger UK cohort of resistant canine lymphomas. Medical records of dogs with resistant non‐Hodgkin high‐grade lymphomas that received DMAC as a rescue protocol were reviewed from 2007 to 2017. Response, time from initiation to discontinuation (TTD) and toxicity (Veterinary Cooperative Oncology Group criteria) were assessed. One hundred dogs were included; 86 received CEOP (modified CHOP including epirubicin) as first‐line treatment. Thirty‐five dogs (35%) responded: 21 complete responders (CRs) and 14 partial responders (PRs). Responders had significantly longer TTD (P < 0.001) compared with non‐responders: 62 days (range 28‐952) for CR vs 32 days (range 20‐70) for PR. Six CR received more than six cycles of DMAC (range 7‐36 cycles) and experienced a longer TTD (median 508, range 126‐952 days). Thrombocytopenia occurred in 45% (24 grade 1‐2, 21 grade 3‐4) and neutropenia in 36% of cases (29 grade 1‐2, 7 grade 3‐4). Gastrointestinal toxicity occurred in 42% of dogs (40 grade 1‐2, 2 grade 3‐4). Owing to chemotherapy toxicity, treatment was discontinued in five, and hospitalization required in six cases. In this study, response to DMAC was lower and of generally shorter duration than previously reported. Toxicity was high, but infrequently led to hospitalization or discontinuation of treatment. 相似文献
22.
This study aimed to investigate the distribution of Malassezia species yeasts on the skin of healthy horses. Acetate tape samples were obtained from the lip, axilla, interbulbar region, groin and anus of 12 healthy horses. The samples were stained and examined microscopically and sites harbouring yeast-like organisms were identified. Contact plates were applied to the skin at these sites and cultured at 26 degrees C and 32 degrees C. No growth was obtained on horse blood, Sabouraud's dextrose or modified Dixon's agar. A pure growth of a Malassezia-type organism was obtained on Sabouraud's dextrose agar enriched with oleic acid when it was incubated at 30 degrees C. It was identified by 26S ribosomal DNA D1/D2 sequence analysis as a member of the genus Malassezia, and most closely related to Malassezia sympodialis. However, the level of sequence divergence indicated that it was a novel species. 相似文献
23.
S Chema S Waghela A D James T T Dolan A S Young W N Masiga A D Irvin G H Mulela L S Wekesa 《The Veterinary record》1986,118(21):588-589
24.
Richards JR Elston TH Ford RB Gaskell RM Hartmann K Hurley KF Lappin MR Levy JK Rodan I Scherk M Schultz RD Sparkes AH 《Journal of the American Veterinary Medical Association》2006,229(9):1405-1441
Vaccination is a medical procedure, and the decision to vaccinate should be based on a risk-based assessment for each cat and each vaccine. 相似文献
25.
Okano S Hurley DJ Bergh MS Vandenplas ML Budsberg SC Moore JN 《Veterinary immunology and immunopathology》2006,112(3-4):234-242
The purpose of this study was to optimize conditions for high throughput measurement of radical oxygen species (ROS) production and expression of tissue factor, also termed procoagulant activity, by canine leukocytes. Granulocytes and mononuclear cells were separated by density gradient centrifugation from peripheral blood collected on several occasions from three healthy large breed dogs. To determine optimal conditions for ROS production, granulocytes were incubated for 1 or 3h in PBG (PBS containing 0.5% BSA and 5mM glucose) or RPMI containing 10% fetal bovine serum (FBS); lipopolysaccharide (LPS), zymosan, peptidoglycan (PGN) and phorbol myristate acetate (PMA) were used as stimuli. ROS was assessed by conversion of the nonfluorescent dye dihydrorhodamine 123 to fluorescent rhodamine 123 by radical species released into the media. To identify optimal conditions for expression of tissue factor, mononuclear cells were incubated for 5h in RPMI containing different concentrations of heat-inactivated FBS (HI-FBS), and LPS, zymosan, PGN or PMA as stimuli. Expression of tissue factor was determined using a one-stage recalcification assay performed in an automated nephelometric coagulation analyzer. Neither LPS nor zymosan increased ROS production by granulocytes incubated in PBG media. In contrast, granulocytes incubated in RPMI had dose-dependent increases in ROS production in response to zymosan and PGN. ROS production was significantly increased by incubation with concentrations of LPS of 0.01microg/ml or greater, and by zymosan concentrations of 0.1microg/ml or greater. ROS production in response to incubation with PMA was significantly increased starting at 10(-7)M, and was significantly greater for cells incubated in RPMI than cells incubated in PBG. LPS-, zymosan- and PGN-stimulated procoagulant activity increased in a dose-dependent manner, whereas PMA-stimulated procoagulant activity peaked at 10(-7)M. Increasing concentrations of HI-FBS significantly increased LPS-, zymosan- and PGN-induced procoagulant activity of mononuclear cells. Results obtained in this study indicate production of ROS by canine granulocytes is optimal when these cells are incubated for 3h in RPMI with LPS (0.1microg/ml), zymosan (10 microg/ml), PGN (10 microg/ml), and PMA (10(-7)M). Furthermore, canine mononuclear cells express procoagulant activity in response to LPS, zymosan, PGN, and PMA, and responses to LPS, zymosan and PGN are enhanced by the addition of HI-FBS. These findings suggest that HI-FBS retains important serum proteins that facilitate interactions between each of these bacterial or yeast derived products and the mononuclear cells. Consequently, future studies regarding the regulation of procoagulant activity by canine mononuclear cells should be performed in the presence of HI-FBS. Both assays utilized in this study allow high throughput of samples, and therefore are appropriate choices for rapid screening of conditions and/or therapeutic interventions affecting the canine inflammatory system. 相似文献
26.
Okano S Hurley DJ Vandenplas ML Moore JN 《American journal of veterinary research》2006,67(6):1020-1024
OBJECTIVE: To determine the effect of fetal bovine serum (FBS) and heat-inactivated FBS (HI-FBS) on lipopolysaccharide (LPS)- and zymosan-induced procoagulant activity of equine and canine mononuclear cells. SAMPLE POPULATION: Mononuclear cells from 18 horses and 3 dogs. PROCEDURES: Cells were incubated with various concentrations of FBS, HI-FBS, LPS, zymosan, polymyxin B, and anti-LPS-binding protein monoclonal antibody or combinations of these constituents. A 1 stage recalcification assay was used to determine procoagulant activity. RESULTS: Addition of FBS to media significantly increased procoagulant activity; equine and canine cells were stimulated by 1% and 10% FBS, respectively. Coincubation of cells with FBS and polymyxin B did not reduce this effect, suggesting that the response was not attributable to LPS contamination. Addition of HI-FBS to media did not stimulate procoagulant activity of equine or canine cells, and the sensitivity of the equine cells to LPS was significantly increased by HI-FBS. This increased LPS sensitivity was reduced 40% with monoclonal antibody directed against human recombinant LPS-binding protein. Increasing concentrations of HIFBS significantly increased LPS- and zymosan-induced procoagulant activity of canine cells. CONCLUSION AND CLINICAL RELEVANCE: Procoagulant activity production in equine and canine mononuclear cells was significantly increased by addition of FBS, whereas heat inactivation of FBS eliminated this effect. Heat inactivation did not eliminate the function of serum proteins involved in enhancement of LPS and zymosan-induced procoagulant activity. Results suggest that HI-FBS can be used as a source of serum proteins that increase the sensitivity of mononuclear cells to bacterial and yeast cell wall components. 相似文献
27.
Prevalence of species and genotypes of Cryptosporidium found in 1-2-year-old dairy cattle in the eastern United States 总被引:6,自引:0,他引:6
The prevalence of Cryptosporidium species in 1-2-year-old heifers was determined for 571 animals on 14 dairy farms in seven states on the East Coast of the United States. A fecal specimen collected directly from each heifer was processed to concentrate oocysts that were then examined by polymerase chain reaction (PCR). For every PCR-positive specimen the 18S rRNA gene of Cryptosporidium was sequenced. Cryptosporidium was identified by PCR from heifers on 13 of 14 farms. On all except four farms groups of heifers were housed in a barn or in large covered pens. Others were pastured. From many of the same farms an earlier study reported that 41% of 393 pre-weaned calves and 26.2% of 447 post-weaned calves were infected. In the present study, 11.9% of 571 heifers were infected with Cryptosporidium, 0.7% with Cryptosporidium parvum, the zoonotic species. Of 68 PCR-positive specimens characterized by gene sequencing 1, 4, 10, 24, and 29 calves were infected with Cryptosporidium suis, Cryptosporidium parvum, Cryptosporidium deer-like genotype, Cryptosporidium bovis, and Cryptosporidium andersoni, respectively. These findings demonstrate a lower prevalence of infection in 1-2-year-old dairy cattle than in younger cattle as well as a change in the diversity of species present. Consequently, the risk of humans acquiring infection with C. parvum from exposure to feces from yearling and older cattle appears much lower than from exposure to pre-weaned calves. 相似文献
28.
S B James R A Cook B L Raphael M D Stetter P Kalk K MacLaughlin P P Calle 《Journal of zoo and wildlife medicine》1999,30(4):521-525
Twelve babirusa (Babyrousa babyrussa) (four females/eight males) were immobilized 30 times during a 4-yr interval. Significantly higher premedication and immobilizing doses were needed for females than for males (P < 0.05). An i.m. preanesthetic xylazine dose of 1.88 +/- 0.37 mg/kg (range = 1.20-2.12 mg/kg) was used for females and 1.22 +/- 0.16 mg/kg (range = 0.82-1.43 mg/kg) for males. After xylazine, the animals were induced with i.m. tiletamine/zolazepam; females received 2.20 +/- 0.47 mg/kg (range = 1.78-3.33 mg/kg) and males received 1.71 +/- 0.34 mg/kg (range = 1.08-2.05 mg/kg). Anesthesia was reversed with yohimbine (0.14 +/- 0.03 mg/kg; range = 0.07-0.20 mg/kg) and flumazenil (1 mg flumazenil/20 mg zolazepam) either i.m. or i.v. This anesthetic combination produced smooth induction, good relaxation, and sufficient immobilization to perform routine diagnostic and therapeutic procedures (venipuncture, hoof and tusk trims, transportation, radiographs, ultrasound examination, weight determinations, and skin biopsies). Supplemental ketamine HCl or isoflurane was administered to two animals to effectively deepen or prolong the anesthetic plane, with no resultant adverse effects. 相似文献
29.
Biffa D Inangolet F Bogale A Oloya J Djønne B Skjerve E 《Tropical animal health and production》2011,43(2):529-538
This study was aimed at investigating risk factors associated with prevalence of tuberculosis (TB)-like lesions and associated
mycobacteria in Ethiopian cattle slaughtered. The study was carried out during 2006–2007 in five selected municipal and export
abattoirs. Methods of investigation involved detailed necropsy examination of carcasses and isolation of mycobacteria from
pathologic tissue specimens. Factors of animal and environment origin were recorded and examined as explanatory variables
in relation to the presence or absence of TB-like lesions and the presence of viable mycobacteria. Multivariable logistic
regression analysis was used to identify risk factors associated with prevalence of TB-like lesions and mycobacteria. Out
of 3,322 carcasses inspected, 10.2% (95% confidence interval (CI) [9.2–11.3]) and 3.2% (95% CI [2.6–3.8]) were positive, respectively,
based on necropsy and bacteriologic examinations. The highest and lowest lesion prevalence was recorded in Adama (24.7%) and
Yabello (4.2%), respectively. Multivariable logistic regression analysis identified age, breed, abattoir location, geographic
origin and management system as being risk factors for prevalence of TB-like lesions and occurrence of viable mycobacteria
in Ethiopian cattle. The study demonstrated mycobacterial infection as important infectious disease of Ethiopian cattle. The
reported confirmed cases of the disease in different management and geographic settings in Ethiopia disproved the earlier
held opinion of its occurrence as a low sporadic profile. In view of a dietary proclivity of Ethiopian communities (milk and
meat are predominantly consumed raw) and lifestyle (close contact of people with animals), the risk of bovine tuberculosis
as a public health threat is eminent. 相似文献
30.