Orchardgrass and perennial ryegrass with applied nitrogen and in mixtures with legumes

High-yielding grass-legume mixtures play an important role in forage-animal systems but finding compatible and adapted species can sometimes be difficult. The objective of this study was to examine productivity of perennial ryegrass and orchardgrass in pure stands with N and in mixtures with legumes.
Broadcast sowings were made on conventionally prepared seedbeds in August 1979 and May 1980 on a Hagerstown silt loam soil (fine, mixed mesic Typic Hapludalf). When sown alone orchardgrass cv. Pennlate and perennial ryegrass cv. Reveille received rates of N ranging from 0 to 448 kg ha⁻¹ a⁻¹. The grasses were also grown in mixtures with alfalfa cv. Arc, red clover cv. Arlington, or birds foot trefoil cv. Viking. The same legumes were sown alone. Four legume seed rates were used in both the pure legume and mixed stands.
Orchardgrass-N swards were more productive than ryegrass-N swards over 3 years. More dry matter (DM) was harvested from pure stands of     

Can heat-pulse sap flow measurements be used as continuous water stress indicators of citrus trees?

Transpiration of well-watered and regulated deficit irrigated (RDI) citrus trees was determined by sap flow (SF) measurements using the compensation heat-pulse method. Its potential for detection of plant water stress was evaluated in comparison with measurements of midday stem water potential (ψ_stem). The study was carried out during 2 years in two commercial groves of Clementina de Nules (CN) and Navel Lane Late (NLL). SF measurements were taken in two trees per treatment instrumented with two identical gauges per tree in NLL and two different types of gauges (type 1 shorter than type 2) in CN. The absolute SF values underestimated the tree water use. Averaged over the entire period of water restrictions, a reduction of about 50 % in water application in the RDI trees of both species decreased tree transpiration compared to the control trees only by a 15 %. Both the nocturnal-to-diurnal SF ratio and the relative transpiration were in good agreement with differences in ψ_stem. Overall, results suggest that SF measurements should be preferentially used in relative terms. Sap flow sensors are useful for detecting plant water stress, but they also highlight some of the problems for accurately measuring transpiration.     

Aetiology of an ulcerative disease in goldfish, Carassius auratus (L.): experimental induction of the disease

Abstract. Infection experiments were conducted to determine the primary aetiology of an ulcerative disease in goldish, Carassius auratus (L.). Goldfish were exposed to atypical Aeromonas salmonicida and A. hydrophila , previousl y isolated from cutaneous ulcers, and to 04 5 μm filtrates of cutaneous ulcers and kidneys from diseased fish. Fish were exposed to each preparation by intraperitoneal, intramuscular or subcutaneous injection and by a method in which a small patch of scales was removed from each side of the fish and the inoculums applied. Most of the fish injected with A. salmonicida died without developing coetaneous ulcers; however, ulcers were induced in five of the ten fish exposed by the scale removal technique. Exposure to ultra filtrates or A. hydrophila did not result in consistent ulcer formation o r death. Additional experiments showed that a 30-min exposure of goldfish, without prior treatment, to water containing 3 × 10⁵ colony forming units (cfu/ml) of A. salnumicida was sufficient to produce cutaneous lesions in nine often fish exposed. Multiple lesions were produced in most fish and A. salmonicida was consistently recovered. Fish exposed by similar waterborne challenge s to 6·2 × 10⁶ cfu/ml of A. hydrophila or to 7·2 × 10⁶ cfu/ml of another lesion isolate identified as a member of the A. hydrophila complex produced no lesions, eve n when scales were removed. The studies demonstrate that atypical A. salmonicida can initiate cutaneous lesion s characteristic of ulcerative disease, while A. hydrophila and an A. hydrophila complex organism cannot.