黄芩地膜露头栽培密度研究

目的:探索地膜露头栽培模式下陇西同类地区黄芩栽培的最佳密度。方法:采用35 cm黑膜覆盖露头栽培模式,在控制统一行距的条件下,设计4种不同的株距进行田间栽培试验,通过观测黄芩地上部分、地下药用部位的生长量、试验区药材产量和种子产量等生物经济指标,结合经济效益分析,优选黄芩地膜露头栽培的最佳密度。结果:株距5 cm栽培的鲜药产量较高,但药材商品规格、产籽量均低,增产不增收;株距15 cm栽培的鲜药商品规格较高,但产量和产籽量均偏低,经济效益低;株距10 cm栽培的出苗率、鲜药产量、产籽量、田间长势、药材商品规格表现好。结论:黄芩35 cm黑膜覆盖露头栽培,在陇西同类地区的最佳密度为行距30 cm、株距10 cm,即亩保苗22 000-22 500株。     

昆明北市林区森林火灾防治方略

文章收集整理昆明北市林区20a(1989～2009)森林防火资料,分析森林火灾发生特点,提出适合该区森林火灾防治方略.     

黑龙江烟草病害种类鉴定及发生情况

为了明确黑龙江主要烟区目前病害发生和危害情况，于2010-2013年对黑龙江烟草主产区病害进行普查。鉴定结果表明，黑龙江烟草病害26种，其中真菌病害11种，细菌病害2种，病毒病害7种，寄生性种子植物1种，非侵染性病害5种；发生普遍且危害重的病害主要是烟草普通花叶病、角斑病。     

腐殖酸与EDTA对高铁酸钾降解水中孔雀石绿影响的研究

在高铁酸钾与孔雀石绿的质量比为10∶1,pH值为7.0的条件下,考察腐殖酸与EDTA对高铁酸钾降解水体中孔雀石绿的影响。结果表明,加入少量的腐殖酸和EDTA溶液,孔雀石绿溶液的吸光度降低,有利于高铁酸钾对孔雀石绿的降解;EDTA的加入量过多时,反而抑制高铁酸钾对孔雀石绿的降解;加入2.0 mL腐殖酸和4.0 mL EDTA溶液降解2 h效果较好。     

草莓FaSKP1-1基因的克隆与表达分析

为研究草莓中SCF复合体的功能,以栽培草莓品种‘74’为试材,采用同源克隆的方法分离出2个SKP1基因。这2个基因核苷酸序列全长均为519bp,核苷酸序列相似性为98.46%,氨基酸序列相似性为98.84%,并具有特殊的‘GVDED’尾巴结构,分别命名为FaSKP1-1a和FaSKP1-1b(基因登录号分别为KU975057和KU975058)。RT-PCR和dCAPS分析发现FaSKP1-1a和FaSKP1-1b在草莓根、茎、叶、花托、花粉、花柱、果实中均高表达,在花瓣中表达量低。上述结果表明FaSKP1-1可能在草莓SCF复合体的形成过程中发挥重要作用。     

Effect of Psammaplin A on in vitro Development of Bovine Aging Oocytes

To investigate the effect of histone deacetylation inhibitor Psammaplin A (PsA) on the development of bovine aging oocytes in vitro,oocytes were randomly divided into control group,aging group and 50 mmol/L PsA treated aging group (PsA group).Immunofluorescence staining and JC-1 were used to detect the blastocyst rate of bovine oocytes after parthenogenetic activation,the number of cells in blastocysts,apoptosis,reactive oxygen species (ROS),glutathione (GSH) and mitochondrial membrane potential intensity of embryos.The results showed that the blastocyst rate of the aging group was significantly lower than that of PsA and control groups (P<0.05).The blastocyst rate of PsA group was not significantly different from that of control group (P>0.05).The number of cells in the blastocysts of control group and PsA group were significantly higher than that of aging group (P<0.05).The number of cells in the blastocysts of PsA group was not significantly different from that of control group (P>0.05).The apoptosis rate in aging group was significantly higher than that of control and PsA groups (P<0.05),the apoptosis rate of PsA group was significantly higher than that of control group (P<0.05).The GSH level of MⅡ oocytes in aging group was significantly lower than that of control and PsA groups (P<0.05).There was no significant difference in GSH level between control and PsA groups (P>0.05).The ROS level of the embryos in aging group was significantly higher than that of control and PsA groups (P<0.05).The ROS level in PsA group was significantly higher than that of control group (P<0.05).The mitochondrial membrane potential of early embryos of aging group 4-8 cells was significantly lower than that of control and PsA groups (P<0.05).The mitochondrial membrane potential intensity of control group was significantly higher than that of PsA group (P<0.05).In summary,PsA could effectively delay the aging of bovine oocytes and improve the quality of oocytes.     

桑园施沼液好处多

随着各地新农村建设不断深入开展,农村旧村改造以及城镇旧城改造进一步扩大,农民人居环境得到了不断改善,从事畜禽生产的农户急剧减少,并逐渐向专业大户养殖发展。农户在种植土地上施用农家肥也越来越少,     

国家级公益林区划成果落界探讨——以兰州市为例

按照国家及甘肃省技术要求、工作流程进行了兰州市国家级公益林区划落界工作,结果为兰州市国家级公益林本期区划落界132611.07 hm^2,剔除了2013年上报中的16104.46 hm^2,在林地类型、区位、权属等方面更符合国家级公益林的条件要求,从地类、生态区位等方面还分析了国家级公益林的面积结构。