60Co辐射诱变姬松茸突变株J3子实体不同部位的氨基酸分析研究

测定并比较了60Co辐射诱变姬松茸突变株J3与原菌株J1子实体不同部位的氨基酸含量.结果表明,除菌柄和盖皮外,姬松茸突变株J3子实体其它部位的必需氨基酸、儿童氨基酸、硫氨酸、支链氨基酸、鲜味氨基酸、芳香族氨基酸和甜味氨基酸均高于原菌株J1.姬松茸突变株J3子实体中谷氨酸含量占氨基酸总量的比例分别比原菌株J1、α-酪蛋白和卵清蛋白提高34.88%、56.68%和122.32%.     

苹果采前落果与内源激素的关系

为了探讨苹果采前落果与内源激素之间的关系,在采前8周间定期测定了不同苹果品种的果柄、果台和离层形成部位组织中IAA、ABA含量;在采收前20d内定期测定了离层部位组织中细胞壁分解酶(Cellulase)的活性;在收获期测定了果实乙烯的发生量。结果表明:不同品种果柄、果台和离层部位组织中IAA和ABA含量变化有差异,但它们变化的总趋势相似,都是随着果实成熟IAA含量下降,而ABA含量上升;不同品种的成熟果实中乙烯发生量有很大差异,以落果多的品种显著大于落果少的品种;采前落果重的品种离层部位组织中细胞壁分解酶的活性在果实成熟期急剧增加。由于果实进入成熟阶段后,IAA含量下降,ABA含量升高,ABA/IAA之间的相对平衡被打破,高ABA/IAA以及高乙烯会刺激离层组织中细胞壁分解酶的活性增高,进而促进离层形成,这可能是导致落果发生的原因。     

香菇出口生产技术综述

香菇无公害生产全程技术监控，包括优化产地生态环境栽培原材料把关，精选优良菌种，菌袋生产规范化，出菇科学管理，控制农药使用，产品标准化等七个方面。     

葡萄子油及原花青素研究与开发利用

综述了葡萄子油、葡萄子原花青素等天然产物的提取与保健作用的研究进展,原花青素的生物活性及药理研究的现状,葡萄子油成分分析方法和葡萄子,提取物中原花青素含量测定的方法;介绍了用超临界CO2萃取法制取高质量的葡萄子油和应用夹带剂提取葡萄子中的原花青素的方法。分析了葡萄子的综合开发的应用前景。     

马铃薯脱毒快繁及微型薯生产技术试验研究

马铃薯,俗名土豆、洋芋、山药蛋,是一种无性繁殖作物,传统的种植方式是将前茬生产的薯块留做后茬当种。由于马铃薯在种植过程中易感染病毒病,随着时代的延续,病毒就会在植株体内增殖、运转、积累于所结的薯块中,使病情逐年加重,通常所说的马铃薯退化现象,就是因为病毒的侵染而造成的产量降低、品质下降。如何去除马铃薯所感染的病毒,作者于2000-2004年进行了试验研究,认为解决这一问题的主要途径是对马铃薯进行脱毒、组培快繁,培育脱毒种薯。一、马铃薯的脱毒方法在我国,寄生于马铃薯上的病毒主要有PVX(马铃薯普通花叶病毒)、PVY(马铃薯…     

极早熟大白菜新品种豫新50的选育

采用游离小孢子培养双单倍体技术快速育成早熟大白菜杂交一代新品种豫新50。结果表明:豫新50为极早熟耐热品种,从播种到采收50-56d,高抗病毒病和软腐病,抗霜霉病;平均667m2产净菜2716.87kg,株形紧凑,适宜密植;叶球矮桩叠抱,整齐度高,商品性好;质地柔嫩,风味佳,每100g鲜样含粗蛋白质1.19g,粗纤维0.40g,维生素C20.8mg,可溶性糖2.80g。     

夏季猪舍屋顶遮阳降温效果研究

本文通过理论分析与试验研究了屋顶遮阳系统在猪舍夏季降温中的应用效果,应用红外热图像摄取及分析系统获取猪舍屋顶内表面和猪体表面的热图像,结果表明屋顶上架设铝箔遮阳网对减少太阳辐射进入猪舍进而降低猪舍环境温度和减轻猪热应激的效果显著。     

Effect of cyclophosphamide on proliferation and apoptosis of rat mesangial cells in vitro

AIM:To investigate the effect of cyclophosphamide(CTX) on proliferation and apoptosis of mesangial cells(GMC) of rat in vitro. METHODS:GMC proliferat ion was detected by MTT method,GMC apoptosis was examined by inverted microscopy for phase-contract and fluoroscopy and flow cytometry analysis.The levels of Fas and Bcl-2 were also detected by immunohistology. RESULTS:The proliferation of GMC were inhibited by CTX, methylprednisolone(MP), low molecular weight heparin(LMWH). Apoptosis of GMC was induced by CTX, the apoptosis rate of GMC was 8.2%, and the Fas level was increased. CONCLUSION:CTX could inhibit proliferation and induce apoptosis of GMC possibly by enhancing the Fas level.     

植物源农用杀菌剂银泰对作物刺激生长作用

本文通过应用植物源农用杀菌剂银泰的不同浓度处理草莓和菠菜，测定绿色面积指数、叶面积指数、根冠比、叶绿素含量、硝酸还原本科活性、生物产量、经济产量。结果表明，银泰对草莓和菠菜均具有明显的刺激生长作用。     

Induction of apoptosis with Salvia miltiorrhiza monomer IH764-3 via downregulating focal adhesion kinase in H2O2-stimulated hepatic stellate cells

AIM: To investigate the effect of IH764-3 on the apoptosis of H₂O₂-stimulated hepatic stellate cells(HSCs) and the alteration of focal adhesion kinase (FAK). METHODS: The proliferation of HSCs was examined by direct cell count and the apoptosis was determined by Annexin-V/PI labeling, while the morphological change was observed by light microscopy and transmission electron microscopy. In addition, FAK mRNA was detected by RT-PCR. RESULTS: H₂O₂ promoted the proliferation of HSCs. IH764-3 induced the apoptosis of HSCs in a dose-dependent manner. The HSC apoptotic rates of different groups were 6. 35%,9. 28%,15. 10%,19. 69%,respectively, after treated with different concentrations of IH764-3 for 48 h while H₂O₂ group showed 2. 30%. In 30 mg/L group, the apoptosis rates were 6. 73%、10. 34%、15. 10% for the indicated time periods(12 h, 24 h, 48 h). In the presence of IH764-3, FAK mRNA decreased. The FAK mRNA reduction began at 2 h after adding IH764-3. CONCLUSION: IH764-3 induced the apoptosis of HSCs. Down-regulating the expression of FAK mRNA may be one of its mechanisms.