我国昆虫病原线虫生物防治应用研究进展

综述了国内昆虫病原线虫的优良品系筛选，防治对象，田间侵染力，耐药性方面的研究。我国已筛选出多个线虫品系，用于田间防治，近期发现小杆科和双胃科的线虫有生防价值。国内研究集中于小卷蛾线虫，芜菁夜蛾线虫和异上杆线虫对40多种害虫的防治，田间防效达70％以上。线虫施用技术，生活习性及搜索方式直接影响其侵染力。应用保湿剂，抗干燥剂及干燥脱水等技术为线虫的储运和防治食叶害虫提供了方便。低毒化学农药不影响线虫的存活和田间侵染力，可以混合使用。我国还应进一步开发利用丰富的线虫资源。     

我国昆虫病原线虫生物防治应用研究进展(综述)

综述了国内昆虫病原线虫的优良品系筛选、防治对象、田间侵染力、耐药性方面的研究.我国已筛选出多个线虫品系,用于田间防治.近期发现小杆科和双胃科的线虫有生防价值.国内研究集中于小卷蛾线虫、芜菁夜蛾线虫和异小杆线虫对40多种害虫的防治,田间防效达70%以上.线虫施用技术、生活习性及搜索方式直接影响其侵染力.应用保湿剂、抗干燥剂及干燥脱水等技术为线虫的储运和防治食叶害虫提供了方便.低毒化学农药不影响线虫的存活和田间侵染力,可以混合使用.我国还应进一步开发利用丰富的线虫资源.     

Variation in essential oil composition within individual leaves of sweet basil (Ocimum basilicum L.) is more affected by leaf position than by leaf age

The aroma in sweet basil is a factor affecting the commercial value of the crop. In previous studies leaf age was considered to be a factor that influences the composition of essential oil (EO). In this study it was hypothesized that a single observation of the EO content in leaves from different positions on the main stem (young vs old) could predict the developmental changes in the plant during its life cycle. Plants harvested at week 16 demonstrated an exponential increase (R(2) = 0.92) in EO concentration in leaves on the main stem and lateral shoots, indicating higher EO concentrations in younger than in older leaves. Eugenol and methyleugenol predominated (28-77%) in the extract. Eugenol levels were higher in younger leaves (～53%), and methyl-eugenol levels predominated in older leaves (～68%). Linalool was lower in mature leaves than in younger leaves. This suggested that eugenol converted into methyleugenol and linalool decreased as leaf mature. However, in weekly monitored plants, the levels of these compounds in the EO had limited variation in the maturing leaf regardless of its position on the stem. This proposed that the EO composition in an individual leaf is mostly affected by the leaf position on the stem and not by its maturation process. Because leaf position is related to plant development, it is probable that the plant's physiological age at the time of leaf formation from the primordial tissue is the factor affecting the EO composition. It was concluded that interpretation of scientific observations should be carried out with caution and that hypotheses should be tested utilizing multifaceted approaches.     

Serum alkaline phosphatase activity is not a marker for neoplastic transformation of esophageal nodules in canine spirocercosis

Background: Spirocerca lupi is a nematode of Canidae that matures within the esophageal wall to form fibroblastic nodules with potential for malignant transformation. Diagnosis is based on histopathologic examination, but false‐negative results may be obtained from samples collected by endoscopy. Serum alkaline phosphatase (ALP) activity, frequently increased in hepatobiliary disease, is also increased in a variety of neoplastic conditions in dogs, including appendicular osteosarcoma, and has also been reported to be increased in dogs with spirocercosis. Objective: The aim of this study was to evaluate serum ALP activity as a marker for malignant transformation of esophageal nodules in S. lupi‐infected dogs. Methods: In this retrospective study, medical records of dogs diagnosed with spirocercosis from 1991 to 2008 were reviewed, and serum ALP activity determined at presentation was compared between dogs with nonneoplastic and neoplastic nodules. Owing to use of multiple analyzers, ratios of ALP activity to the upper reference interval for ALP were calculated and compared. Results: Median ALP activity ratios were 0.65 (0.07–4.00) and 0.86 (0.10–3.40) for dogs with nonneoplastic (n=88) and neoplastic (n=32) nodules, respectively, with no significant difference (P=.18) and substantial overlap between groups. Tumors included osteosarcoma (15 dogs), fibrosarcoma (15 dogs), and anaplastic sarcoma (2 dogs); there was no difference in ALP activity between the dogs with osteosarcoma and fibrosarcoma. Conclusion: ALP is a poor marker of malignant transformation in canine spirocercosis.     

The influence of connectivity on richness and temporal variation of reef fishes

To test the effects of connectivity on fish diversity in isolated reef patches we deployed pairs of artificial reefs (AR) at constant distances (12 and 25 m) from a large continuous reef and added series of small ARs to half of them. These small reefs served as stepping-stones to increase fish movement between the large ARs and the continuous reef. Species gain and loss curves were compared to obtain deeper understanding of the underlying mechanisms in relation to the theory of island biogeography. We found that AR without stepping-stones maintained up to 57-fold more individuals than ARs with stepping-stones during mass recruitment events that were dominated by the family Apogonidae. However, in-between these mass recruitment events the overall impact of the stepping-stones on richness and abundance was small. By contrast, increasing distance from the continuous reef from 12 to 25 m increased fish richness, although this was confounded with time since AR deployment. Increase in richness with distance was predominantly caused by elevated species gain. Our results suggest that fish assemblages respond in distinct ways to two types of isolation: (1) Increased richness with distance from a continuous reef, and (2) increased fluctuation in abundance with decreased connectivity. The latter may be the outcome of accumulation and then abrupt emigrations of fishes from isolated reefs. This study confirms that the spatial setting of reef patches in relation to larger or continuous reefs alters fish assemblages, which may be important for planning AR deployments that maximizes diversity.     

Transient megaoesophagus and oesophagitis following diaphragmatic rupture repair in a cat

A 6-month-old domestic shorthair female cat was presented with suspected diaphragmatic hernia (DH) that was later confirmed by thoracic radiography. The cat underwent exploratory celiotomy with a diaphragmatic rupture (DR) repair and recovered. Six days later, it was represented with vomiting and anorexia. Megaoesophagus (MO) and gastric dilatation were diagnosed by contrast radiography. A second celiotomy revealed no abnormalities and gastropexy was performed. Endoscopy demonstrated MO, oesophagitis and gastro-oesophageal reflux. MO persisted for several weeks and was an unexpected complication as no association between DR (or DH) and MO has never been described in the veterinary literature. The cat was treated medically with aggressive prokinetic and antacid therapy along with prolonged temporary oesophageal diversion (percutaneous endoscopic gastrostomy tube) with an excellent outcome.     

Marked subcutaneous mast cell and eosinophilic infiltration associated with the presence of multiple Dirofilaria repens microfilariae in 4 dogs

Dirofilaria repens is a parasitic nematode in the subcutaneous tissue of carnivores, including dogs and cats, transmitted by mosquitoes. Human beings may be accidental hosts. Infection of a dog with D repens was first reported in Palestine in 1934, and 2 additional cases were reported in dogs in Israel to date. This report describes D repens infection in 4 dogs in Israel that presented with subcutaneous masses, which were cytologically characterized by marked mast cell and eosinophil infiltration. In 3 cases, multiple microfilariae were present in the lesions; rare microfilariae were present in the 4^th case. In all 4 dogs, PCR of fine‐needle aspirates from the lesions were positive for D repens. The mast cells observed in all lesions were uniform and highly granulated, and with the presence of the microfilariae, a mast cell tumor was considered less likely. This report suggests that D repens infection‐associated subcutaneous lesions, characterized cytologically by massive mast cell and eosinophil infiltration, should be considered a differential diagnosis for mast cell tumor, especially in geographic locations endemic for this nematode. Notably, all 4 dogs were infected with D repens despite a routine prophylactic doramectin therapy administered every 3 months, probably due to the relatively long time interval between treatments.     

Coinfection with multiple tick-borne and intestinal parasites in a 6-week-old dog

Coinfection with Ehrlichia canis, Babesia canis, Hepatozoon canis, Isospora spp., Giardia spp., and Dipylidium caninum were detected in a 6-week-old dog. The effect of multi-pathogen infection was a fatal combination of gastrointestinal and hematologic abnormalities, including diarrhea, vomiting, anorexia, distended painful abdomen, intussusception, severe thrombocytopenia, anemia, and hypoproteinemia.