玉米种子增铁、锰、钼包衣对幼苗生长影响

以玉米品种‘三峡玉3号’为试验材料,采用盆栽方法,分析玉米种子进行增铁(Fe)、锰(Mn)、钼(Mo)包衣对玉米幼苗性状生长的影响。结果表明：Fe、Mn、Mo能促进玉米幼苗生长,不同的微量元素增微包衣玉米幼苗生长有差异,Fe和Mn对玉米幼苗生长影响作用大于Mo。除主根条数外,玉米各幼苗性状随着增微包衣浓度的增加呈现“低-高-低”的变化趋势,表现为二次函数关系(y=c+bx+ax²)。筛选出适合玉米幼苗生长的最佳Fe、Mn、Mo 3种微量元素种子包衣浓度分别为474.83~1163.45、100~500、386.40~649.04 mg/kg。     

秸秆深施对土壤蓄水能力的影响

对耕地进行秸秆深施,可提高土壤的入渗速率,增加土壤蓄水空间和能力,提高降水的利用率,是缓解干旱半干旱地区农田的水分匮乏的有效措施。将厚度为8cm的大豆秸秆、玉米秸秆和水稻秸秆分别深施于宽5cm、深28cm的沟中并回填土壤,研究其蓄水效果。结果表明,秸秆深施后,地表下0～20cm的土壤含水量比对照地提高了0.5%～2.0%,深度越接近秸秆深施处土壤含水量越大,反之越小,深施玉米秸秆和水稻秸秆的蓄水效果与对照地有显著差异(P0.05),而深施大豆秸秆的蓄水效果无显著差异(P0.25)。     

不同培养基对小麦纹枯病菌生长繁殖及致病力的影响

采用不同的培养基培养小麦纹枯病病菌,结果表明:在改良的PDA培养基、PDA培养基 小麦叶汁培养基、V8培养基和番茄培养基上培养的病原菌比在其他的培养基上培养的病菌菌丝生长速度快,且其对3种小麦品种的致病力较其他培养基上的病菌强,因此可用这四种培养基复壮、保存小麦纹枯病病菌。     

Comparison of morphology, viability, and function between blood and milk neutrophils from peak lactating goats

The morphological features of blood and milk neutrophils from peak lactating goats were compared using light microscopy, scanning electron microscopy and flow cytometry in order to investigate the cytological changes of neutrophils after migration into the mammary gland. The kinetics of reactive oxygen intermediates (ROI) generation and gelatinase release of blood and milk neutrophils, with or without stimulation of phorbol 12-myristate, 13-acetate ester (PMA), were used to characterize their responses to inflammatory stimuli. Neutrophils isolated from goat milk were highly segmented and contained multi-lobed nuclei. Ultrastructurally, milk neutrophils were more ruffled on the surface compared to blood neutrophils. Approximately 30% of milk neutrophils were undergoing cell death, either necrosis or apoptosis, in contrast to 8% of blood neutrophils. The ROI production of activated milk neutrophils peaked earlier than blood neutrophils, but the duration and the intensity were much less. Neutrophils from both sources augmented the release of gelatinase in response to PMA (1 ng/mL). However, the amount of gelatinase released from milk neutrophils was lower (P < 0.05) than that of blood neutrophils. In summary, more neutrophils become apoptotic and necrotic in the mammary gland, presumably due to spontaneous aging, the process of diapedesis, and the interaction with milk components. Milk neutrophils have impaired functionalities in comparison with blood neutrophils. The information is relevant when studying mammary gland immunity and related diseases, such as mastitis.     

Induced ovulation of yellow catfish (Pelteobagrus fulvidraco) using a combination of a gonadotrop‐releasing hormone analogue and domperidone

The effects of an intraperitoneal hormone injection of gonadotropin‐releasing hormone agonist (D‐Ala⁶, Pro⁹‐NEt GnRHa) alone or in combination with a dopamine antagonist, domperidone (DOM), on ovulation induction in yellow catfish Pelteobagrus fulvidraco were tested. The hormone treatments were as follows: 6 mg kg⁻¹ body weight (BW) of carp pituitary extract as a positive control, GnRHa 10, 20, 40 and 80 μg kg⁻¹ BW and a combination of GnRHa and DOM as follows: 10 μg+5 mg, 20 μg+10 mg, 40 μg+20 mg and 80 μg+40 mg kg⁻¹ BW. Physiological saline (0.7% NaCl) was used as a negative control. Significant differences in the ovulation ratio, latency period and ovulation index (OI) were observed among treatments (P<0.05). The combination of GnRHa and DOM at doses of 40 μg+20 mg kg⁻¹ BW had higher values of the ovulation ratio and OI, and a shorter latency period compared with other treatments. The highest OI in GnRHa treatments was only 56.67%, suggesting a dopaminergic tone on gonadotropin secretion in this fish at the pre‐ovulatory stage. Therefore, ovulation can be successfully induced in yellow catfish with 40 μg kg⁻¹ GnRHa+20 mg kg⁻¹ DOM without affecting the egg quality.     

景宁县白茶品系性状品质比较研究

白茶是我国特有的茶树品种资源。通过对景宁白茶6个品系性状特征观测比较,春茶生化品质检测和加工产品感官评审,为该县茶叶品质提升和白茶开发利用提供基础资料。     

河南省及周边地区猪圆环病毒2型分子流行病学调查

为了解河南及周边地区猪圆环病毒2型(PCV2)的分子流行病学情况,运用PCR方法对2014—2015年采集到的河南及周边地区山东、山西、河北、甘肃5省共158份病料进行PCV2检测,将PCR扩增得到的PCV2 ORF2基因片段进行测序,分析研究PCV2遗传变异情况。结果显示,158份样品中,有69份样品为PCV2阳性,得到19株病毒的ORF2基因序列;将检测到的PCV2 ORF2序列与国内外分离株进行比对发现,核苷酸序列的同源性为89.9%~99.9%,其氨基酸序列同源性为88.0%~99.6%。综上可知,河南及周边地区PCV2流行广泛,病毒变异频繁。     

犬4种皮肤病的诊治

北京市密云县某社区统计饲养宠物犬约375只,其中约200只能够得到精心饲养,定期进行洗浴。调查发现,在定期洗浴的所有犬中,仅有少部分患有轻微皮肤病,个别患病严重;未定期洗浴的犬中,患皮肤病的现象十分普遍,并且相当一部分病情严重。在动物疫病预防控制中心就诊的23个病例中,犬被螨虫感染或真菌感染的概率都很高,分别为10/23、8/23,比例相当。犬同时被2者感染的概率较低,为4/23。其中,雄性患病率13/23;雌性患病率10/23,并未发现犬的皮肤病与性别有关。经过用药治疗,在2周内康复的病例为3例,第3周康复的为7例,4周以后康复病例较多,但其中1例继发感染,死于败血症。     

国外X80管道钢管的研究与应用现状

综述了国外在高钢级X80管道钢管方面的开发和应用状况，管材的研究目前正向更高屈服强度、更高韧性和易加工的方向发展，使用高钢级管材是提高管道设计压力的主要途径，同时，在同样的设计压力下使用高强度管材可养活钢材的消耗量。以德国Ruhrags管道为例，介绍了国外高钢级管道钢管X80的实际质量水平，包括管道钢的合金化、控制轧制和控制冷却工艺，并就高钢级管道钢管的部分热点问题如富气输送、高钢级管道钢管的焊接和裂纹扩展止裂等进行了讨论和分析。     

北海道9号苹果在冀北山地引种初报

为探讨北海道９号苹果在冀北山地推广的可能性，于１９９５￣１９９７年进行了引种观察，积累了试验数据。结果表明，北海道９号苹果抗寒性较强，丰产性能好，果实着色好，品质优良，可在冀北平泉县中部及其以南的区域进一步进行生产示范，建园方式采用以国光／海棠作基砧，用高接换头方法较好。