侵染马铃薯的烟草坏死病毒的鉴定

 1987年自东北农学院马铃薯区域试验材料(东农85-3334)上分离到烟草坏死病毒(Tobacco Necro-sis Virus简称TNV)。     

棉花幼苗侧根发生的化学诱导研究 Ⅲ.Ca~(2+)对棉花幼苗侧根发生和根系IAA含量的影响研究初报

清水或 DPC 200ppm 溶液浸种培养的棉花幼苗在移栽时用含 Ca~(2+)30ppm 的 CaCl_2溶液漫根2小时处理,栽后8天观察发现,两种浸种方式,Ca~(2+)均能促进棉苗侧根的发生,并提高根系 IAA 含量。但 Ca~(2+)处理 DPC 降低根对棉酚含量的效应无明显影响。上述试验结果显示出 Ca~(2+)与诱导棉苗侧根发生和调节根系 IAA 水平之间存在着某些内在的联系,这可能涉及到 Ca~(2+)·CaM系统(胞内第二信使系统)与植物激素效应之间的关系。     

Effect of mitochondrial ATP sensitive potassium channels opening on translocation of protein kinase C epsilon in cultured adult rat ventricular myocytes

AIM: To investigate the effects of mitochondrial ATP sensitive potassium (MitoKATP) channel opening on translocation of protein kinase C epsilon (PKCε) and the relationship between the translocation of PKCε and the production of reactive oxygen species. METHODS: The expression of PKCε in cultured adult rat ventricular myocytes was investigated with immunofluorescence and Western blotting techniques. RESULTS: （1） Diazoxide, a selective MitoKATP channel opener, caused a significant translocation to myofibrillar-like structures in cultured adult rat ventricular myocytes. (2) The N-2-mercaptopropionylglycine (MPG), a free radical scavenger, partly inhibited the translocation of PKCε caused by diazoxide. (3) Chelerythrine, a selective protein kinase C (PKC) inhibitor, completely blocked the translocation of PKCε caused by diazoxide. CONCLUSION: Opening of MitoKATP channel might activate PKCε and make it translocation to myofibrillar-like structures. PKCε activation occurs downstream of MitoKATP channel, and might be caused by production of reactive oxygen species after opening of MitoKATP channel.     

促进4种野生花卉种子萌发的方法研究

野生花卉金莲花(Trollius chinensis)、牛扁(Aconitum barbatum vat.puberulum)、野罂粟(Papaver naudicaule)和草本威灵仙(Veronicastrum sibiricum)发芽率低,采用不同的温度、赤霉索浓度和光周期组合处理,明显提高了这些花卉种子的发芽率和发芽势,加快种子萌发进程.结果表明:(1)采用500 ms/L GA3,25℃和8 h光照16 h黑暗组合处理,可以使金莲花种子发芽率由对照的4%提高到84%;(2)采用500 ms/L GA3,15℃、12 h光照12 h黑暗组合处理,可以使牛扁种子萌发率由对照的20%提高到82.4%;使草本威灵仙种子发芽率由对照的17.3%提高到100%.(3)采用125mg/L GA3,15℃,24 h光照萌组合处理,可以使野罂粟种子萌发率由对照的29.3%提高到61.3%.     

白木香解剖构造及抽提物化学成分分析

以内含物不同的白木香样品为研究对象,对比分析其微观构造和离析组织结构,研究了白木香抽提物的主要化学成分变化,探讨了其结香机理。结果表明：白木香的树脂分布在木射线薄壁细胞及内含韧皮部,在内含韧皮部中伴胞为合成树脂的主要细胞,筛管等为辅助细胞;白木香结香是由于木射线、伴胞等薄壁细胞受到胁迫的生理机能作用所形成,与木材心材的形成机制相同;抽出物与木质素含量变化均为白木香受到刺激后产生的现象,灰分含量与抽出物含量呈负相关,间接说明白木香结香与木质部中的水分含量有重要关系。     

牛磺酸的化学合成方法及应用前景

牛磺酸具有广泛的药用和保健功能。综述了牛磺酸的化学合成方法,并分析了牛磺酸的应用前景。     

焚烧对芨芨草滩地草场土壤生态条件的影响

本文研究了焚烧对芨芨草滩地草场上壤生态条件的影响。结果表明，焚烧可使土壤温度升高，地表可升温3．2～10．8℃，地表以下土层也有所升温，但其数值随深度增加而递减。焚烧后土壤含水量在0～40cm土层内可增加2．42～4．58％，土壤含盐量和土壤含磷量均有增加，但土壤含氮量则有所下降。     

The long-term effects of thinning and mixing on species and structural diversity of Chinese fir plantations

New Forests - Plantations are playing an increasingly important role in providing forest products and improving the ecological environment, but they also face many challenges, such as low...     

桑树TrnL-trnF基因间隔区序列的特点及分析

设计桑树TrnL trnF基因间隔区序列的特异引物 ,扩增并分析了桑属育 71 1和桑莲 2个品种的TrnL trnF基因间隔区序列 ,其长度分别为 4 14bp和 4 17bp ,且富含A/T。该序列有 4 2 1个分析性状 ,具有 17个变异位点。在这些变异中主要以碱基的插入和缺失 (主要是插入 /缺失dA)为进化形式 ,其余的发生了少数置换。除这些变异位点以外的核苷酸序列完全相同 ,说明两者有高度同源性 ,与桑科、菊科和蔷薇科的TrnL trnF基因间隔区序列有一定同源性。用Clustalx软件对 2 1份材料的TrnL trnF基因间隔区序列进行聚类分析 ,建立了系统进化发育树     

Identification of quantitative trait loci for agronomic and physiological traits in maize (<Emphasis Type="Italic">Zea mays</Emphasis> L.) under high-nitrogen and low-nitrogen conditions

Low-nitrogen (LN) tolerance is a compound character with a complex genetic basis. Many agronomic traits have been shown to be closely related to LN tolerance in maize. In this study, 150 F₇ recombinant inbred lines derived from a cross between inbreds 178 and K12 were evaluated for agronomical and physiological traits under high-nitrogen (HN) and LN conditions in 2 years. Inclusive composite interval mapping (ICIM) was used to identify the quantitative trait loci (QTLs) for traits recorded under different treatments (LN and HN) in 2 years. In total, 86 QTLs were detected: 38 for HN and 35 for LN, while 13 QTLs were detected under both nitrogen levels, suggesting that LN-specific QTLs may play a role in improving LN tolerance in maize. Overlapping QTLs for different traits were located on all chromosomes except chromosome 4 and chromosome 9. Many of these regions overlapped with previously reported QTLs. Several consensus major QTLs and LN-specific major QTLs found in the study can be used in marker-assisted selection breeding for genetic improvement and LN tolerance in maize in the future.