针对红锥速生丰产造林技术探讨

红锥作为一种重要的造林树种,其种植受到人们的广泛重视.提升红锥的速生丰产造林技术水平,对于农民的增产增收具有重要的作用.笔者采取实验的方式,对红锥速生丰产造林技术进行了研究.     

从空间尺度论述陆生野生动物生境选择研究进展

指出了生境是动物生存和繁衍的重要场所,生境的质量及其可利用程度是决定动物种群生死存亡的关键因素。因此,研究动物的生境利用特点成为生物保护学研究的热点。综述了不同空间尺度上陆生野生动物生境选择研究的概况,以期对今后开展生境利用研究工作时在方法的选取方面提供借鉴和指导。     

福建柏自然资源调查研究及建议

调查了福建省内外的福建柏自然资源,分析了福建柏自然资源的分布情况及其现状,并针对保护工作提出了一些对策及建议。     

简述方正林业局第五经理期企业经营状况

经过走访调研,对黑龙江省森工林区方正林业局在第五经理期企业的经营状况进行了简要归纳和介绍,旨在为该局今后经济社会发展提供借鉴和帮助。     

轮圈式链轮设计理论的研究

本文提出链锯链轮与锯链正确啮合条件的新理论。对于小节距型号的链锯锯链确定了不同的几何参数作为原始数据 ,并在正确啮合条件下建立了一套适用于不同形式小节距锯链的轮圈式链轮设计理论公式。     

Event-specific quantitative detection of nine genetically modified maizes using one novel standard reference molecule

With the development of genetically modified organism (GMO) detection techniques, the Polymerase Chain Reaction (PCR) technique has been the mainstay for GMO detection, and real-time PCR is the most effective and important method for GMO quantification. An event-specific detection strategy based on the unique and specific integration junction sequences between the host plant genome DNA and the integrated gene is being developed for its high specificity. This study establishes the event-specific detection methods for TC1507 and CBH351 maizes. In addition, the event-specific TaqMan real-time PCR detection methods for another seven GM maize events (Bt11, Bt176, GA21, MON810, MON863, NK603, and T25) were systematically optimized and developed. In these PCR assays, the fluorescent quencher, TAMRA, was dyed on the T-base of the probe at the internal position to improve the intensity of the fluorescent signal. To overcome the difficulties in obtaining the certified reference materials of these GM maizes, one novel standard reference molecule containing all nine specific integration junction sequences of these GM maizes and the maize endogenous reference gene, zSSIIb, was constructed and used for quantitative analysis. The limits of detection of these methods were 20 copies for these different GM maizes, the limits of quantitation were about 20 copies, and the dynamic ranges for quantification were from 0.05 to 100% in 100 ng of DNA template. Furthermore, nine groups of the mixed maize samples of these nine GM maize events were quantitatively analyzed to evaluate the accuracy and precision. The accuracy expressed as bias varied from 0.67 to 28.00% for the nine tested groups of GM maize samples, and the precision expressed as relative standard deviations was from 0.83 to 26.20%. All of these indicated that the established event-specific real-time PCR detection systems and the reference molecule in this study are suitable for the identification and quantification of these GM maizes.     

Improvement of texture properties and flavor of frozen dough by carrot (Daucus carota) antifreeze protein supplementation

The effects of concentrated carrot protein (CCP) containing 15.4% (w/w) carrot (Daucus carota) antifreeze protein on texture properties of frozen dough and volatile compounds of crumb were studied. CCP supplementation lowered the freezable water content of the dough, resulting in some beneficial effects including holding loaf volume steadily and making the dough softer and steadier during frozen storage. Furthermore, SPME-GC-MS analysis showed CCP supplementation did not give any negative influences on volatile compounds of crumb and gave a pleasant aroma felt like Michelia alba DC from trans-caryophyllene simultaneously. Combining our previous results that CCP supplementation improves the fermentation capacity of the frozen dough, CCP could be used as a beneficial additive for frozen dough processing.     

Flumorph Is a Novel Fungicide That Disrupts Microfilament Organization in Phytophthora melonis

ABSTRACT The mechanism of the effects of flumorph (a novel fungicide) was investigated by analyzing alterations of hyphal morphology, cell wall deposition patterns, F-actin organization, and other organelles in Phytophthora melonis. Calcofluor white staining suggested that flumorph did not inhibit the synthesis of cell wall materials, but disturbed the polar deposition of newly synthesized cell wall materials during cystospore germination and hyphal growth. After exposure to flumorph, zoospores were able to switch into cystospores accompanied with the formation of a cell wall, whereas cystospores failed to induce the isotropic-polar switch and did not produce germ tubes but continued the isotropic growth phase. In flumorph-treated hyphae, the most characteristic change was the development of periodic swelling ("beaded" morphology) and the disruption of tip growth. Newly synthesized cell wall materials were deposited uniformly throughout the diffuse expanded region of hyphae, in contrast to their normal polarized patterns of deposition. These alterations were the result of F-actin disruption, identified with the fluorescein isothiocynate (FITC)-phalloidin staining. The disruption of F-actin also was accompanied by disorganized organelles: each swelling of subapical hyphae was associated with a nucleus. Vesicles did not undergo polarized secretion to the apical hyphae, but diffused around nuclei for the subapical growth; thus, the cell wall was thickened with periodic expansion along the hyphae. Upon removing flumorph, normal tip growth and organized F-actin were observed again. These data, as well as data published earlier, suggest that flumorph may be involved in the impairment of cell polar growth through directly or indirectly disrupting the organization of F-actin. The primary site of action by flumorph in the disruption of the F-actin organization is under investigation.     

Rapid evolution of Ophraella communa cold tolerance in new low-temperature environments

Low winter temperatures severely stress newly arriving insect species. Adaptive evolutionary changes in cold tolerance can facilitate their establishment in new environments. Ambrosia artemisiifolia, a noxious invasive plant, occurs throughout China. Ophraella communa, a biological control agent of A. artemisiifolia, mainly occurs in southern China. However, in 2012, it established populations in Beijing (39.98°N, 115.97°E) following introduction from Laibin (23.62°N, 109.37°E), implying cold adaptation. The mechanisms underlying its rapid evolution of cold tolerance remain unknown. We investigated the levels of cryoprotectants and energy reserves in adult O. communa from two latitudes. In high-latitude insects, we found high trehalose, proline, glycerol, total sugar, and lipid levels; five potential genes (Tret1a, Tret1b, Tret1-2, P5CS, and GST), responsible for regulating cold tolerance and involved in trehalose transport, proline biosynthesis, and glutathione S-transferase activation, were highly expressed. These hybridisation changes could facilitate cold temperature adaptation. We demonstrate the genetic basis underlying rapid adaptation of cold tolerance in O. communa, explaining its extension to higher latitudes. Thus, specialist herbivores can follow host plants by adapting to new temperature environments via rapid genetic evolution.

     

武汉市农田林网白蚁生存环境与防治技术研究初探

白蚁是危害武汉市农田林网的主要害虫之一,对全市立木、农作物、建筑和木材谷物等造成很大损失。本研究调查了武汉市主要农田林网地区白蚁的种类、生物学特性及活动生存环境,并分析了种群与土壤之间的关系。结果显示项目研究地农田林网树木白蚁主要为黑翅土白蚁,喜欢生活在潮湿的土壤中。在粘壤土中,主巢入土深度在150 cm以内的占总数80.2%。通过毒饵诱杀法和药物阻隔法,市售的三种药剂均对黑翅土白蚁具有较好的防治效果,平均防治效果达到80.0%以上。