Comparison of quantitative immunoturbidimetric and semiquantitative latex‐agglutination assays for D‐dimer measurement in canine plasma

Background: D‐dimer measurement in dogs is considered the most reliable test for detecting disseminated intravascular coagulation or thromboembolism. Objectives: The purposes of this study were to compare 2 D‐dimer assays, a quantitative immunoturbidimetric and a semiquantitative latex agglutination assay, and to assess the effect of hemolysis and storage conditions on D‐dimer concentration using the quantitative assay. Methods: The immunoturbidimetric assay was validated using canine citrated plasma samples containing different concentrations of D‐dimer. The effect of storage at various temperatures and times was assessed. Hemolysis was produced by adding lysed RBCs to the samples for a final hemoglobin concentration of 0.35 g/dL. Results: For clinically relevant values (>250 μg/L), intra‐assay and interassay coefficients of variation were 6.8% and 7.2%. The assay was linear (r²=1.00), and the tests had good agreement (κ=0.685, P<.001). Storage at 4 °C and ?20 °C and hemolysis had no significant effect on D‐dimer concentrations. In hemolyzed samples stored at room temperature for ≥48 hours, fine clots were noted and often resulted in falsely increased D‐dimer concentrations. Conclusions: Our findings suggest that the immunoturbidimetric assay validated in this study is reliable and accurate for the measurement of D‐dimer in canine plasma. Samples can be stored for up to 1 month at ?20 °C and moderate hemolysis does not significantly affect the D‐dimer concentration in frozen or refrigerated samples.     

Zuni farming and united states government policy: The politics of biological and cultural diversity in agriculture

Indigenous Zuni farming, including cultural values, ecological and biological diversity, and land distribution and tenure, appears to have been quite productive and sustainable for at least 2000 before United States influence began in the later half of the 18th century. United States Government Indian agriculture policy has been based on assimilation of Indians and taking of their resources, and continues in more subtle ways today. At Zuni this policy has resulted in the degradation and loss of natural resources for farming, reduction in the number of Zuni farmers and their control over farming resources, individualization of rights in farmland, consolidation of farm fields, and declining biological diversity in agriculture. The Zuni Sustainable Agriculture Project with the Zuni Irrigation Association and the Zuni community, are now working to revitalize sustainable Zuni farming, based on traditional values, knowledge, and technology, combined with modern knowledge and technology where appropriate. The United States government can support these efforts through appreciation of the need for Zuni control and the potential value of cultural and biological diversity.     

Effect of nitrogen and potassium fertilizer on growth,herbage and oil yield of irrigated palmarosa (Cymbopogon martinii [roxb.] wats. var. motia burk) in a semi-arid tropical climate

The world's agricultural production is increasing by 1 to 2 per cent annually and has been enough to satisfy the growing world population's need for food. Breeding, fertilisation and crop protection have decidedly contributed to that progress in productivity. But since the 1960s, agriculture has been more and more conceived by the public as an environment-contaminating industry. The public is concerned about water contamination with nitrate as a result of the use of fertilisers, and about harmful effects of chemical plant protection products on soil, water, air, animals and wild plants. The public discussion has not remained without consequences. Sustainability of development has become a central political objective in all spheres of social life, and in all world regions. Sustainable development concerns economic, ecological as well as social objectives, and is also the guideline for a renovation of agriculture. Any concepts and strategies integrating environmental aspects into agricultural production are linked with the objective of sustainable development. Discussion on sustainable agriculture has become more concrete on the basis of the Brundtland Report and Agenda 21 , as well as the various follow-up meetings of the 1992 Conference of Rio. Although efforts towards sustainable development in agriculture encounter widespread scepticism, clear progress is to be noted since that discussion has intensified. Farmers have paid more attention to new farming practices. Environmental aspects now play an important role when extension services advise farmers on fertilisation and crop protection. Research has contributed essentially to the change in agriculture towards sustainable production. Sustainability in agriculture has also been expressed in new legal regulations. Principles of good professional practice in applying fertilisers, as it is required by the Fertilisers Act from the year 1989, have been spelled out more clearly in a legal regulation issued on 26 January 1996 (Federal Law Gazette part I of 06 February 1996, p. 118). Principles of good professional practice in crop protection were published in Bundesanzeiger (Federal Gazette) No. 220 a of 21 November 1998. Principles of integrated crop protection were first discussed by representatives of agriculture, environment protection offices and researchers at a workshop at Biologische Bundesanstalt für Land- und Forstwirtschaft (BBA, Federal Biological Research Centre for Agriculture and Forestry) in Kleinmachnow on 23° March 2000. The debate on sustainability centres around the aspects of economic and social efficiency of measures taken, and their effects on the environment. The following article deals with some basic aspects of efficiency on the one hand, and effects/emission on the environment on the other, of fertilisers and crop protection products as important input factors in agriculture. Crop husbandry always is an interference with nature. The Lüneburger Heide cropping region is a memorial of ecologically out-dated land management. Today, sustainable and at the same time efficient agriculture can only be realised with modern methods based on sound knowledge. This includes mineral fertilisation and chemical crop protection. Cropping permanently draws nutrients from the soil-plant system. They have to be refilled by fertilisation. If no fertilising measures are taken at all, the humus layer of the soil declines and soil degrades. The decline in fertility will result in yield losses of between 70 and 90%. In contrast, combination of organic and mineral fertilisers has continually added to soil fertility. This is reflected in the humus content of the soil, which is by about a quarter higher than it was a hundred years ago. Farmers resorting solely to mineral fertilisation produce yields lower by 5-10% than those combining mineral and organic fertilisers. If only organic fertilisers are used, this may reduce yields by between 10 and 40%, depending on the site. Unwanted side-effects of fertilisation (such as leaching of nitrates) may be reduced to a tolerable level by precise input of nutrients, improved methods such as precise fertiliser input depending on the condition of certain field patches, and by integrated crop protection, which relies on synergetic effects of various measures. Germany has for these reasons witnessed a relaxation of environmental pressure from excessive nutrient input. If crop protection measures were abandoned, pests and diseases would reduce yields by between 40 and 80 per cent. Our own trials about energy input-output proportions have shown a ratio of 1:4.6 with use of fertilisers (100kg N/ha), and a ratio of 1:8.6 with use of fertiliser and chemical plant protection products. The risk potential of modern chemical plant protection products has permanently decreased over the past few decades and is generally about one tenth of the values of 15 years ago. Integrated crop protection, which combines various methods of crop protection and reduces the use of chemical crop protection products to a necessary minimum, is the "optimal solution for the future" and "contributes to the sustainability of agriculture", as it is said in the Agenda 21 programme adopted by the 1992 environmental conference in Rio.     

Description and validation of a magnetic resonance imaging-guided stereotactic brain biopsy device in the dog

A stereotactic brain biopsy system that is magnetic resonance (MR) imaging-guided has not been validated in dogs. Our purpose was to determine the mean needle placement error in the caudate nucleus, thalamus, and midbrain of a canine cadaver brain using the modified Brainsight stereotactic system. Relocatable reference markers (fiducial markers) were attached to the cadaver head using a dental bite block. A T1-weighted gradient echo three-dimensional (3D) sequence was acquired using set parameters. Fiducial markers were used to register the head to the acquired MR images in reference to a 3D position sensor. This allowed the planning of trajectory path to brain targets in real time. Coordinates (X, Y, Z) were established for each target and 0.5 microl of diluted gadolinium was injected at each target using a 26-gauge needle to create a lesion. The center of the gadolinium deposition was identified on the postoperative MR images and coordinates (X', Y', Z') were established. The precision of this system in bringing the needle to target (needle placement error) was calculated. Seventeen sites were targeted in the brain. The mean needle placement error for all target sites was 1.79 +/- 0.87 mm. The upper bound of error for this stereotactic system was 3.31 mm. There was no statistically significant relationship between needle placement error and target depth (P = 0.23). The ease of use and precision of this stereotactic system support its development for clinical use in dogs with brain lesions > 3.31 mm.     

Effects of a Rest–Rotation Grazing System on Wintering Elk Distributions at Wall Creek,Montana

The large-scale influence of livestock grazing in the western United States generates a need to integrate landscape management to incorporate both wildlife and livestock. The purpose of this project was to evaluate the effects of four different grazing cells (spring grazing, summer growing-season grazing, fall grazing, and resting) on wintering elk resource selection within the Wall Creek range in southwest Montana. We collected biweekly observations of elk (Cervus elaphus) numbers and distributions across the winter range from 1988 to 2007. Using a matched-case control logistic regression model to estimate selection coefficients, we evaluated the effects of annual green-up conditions, winter conditions, landscape features, and grazing treatment on elk group resource selection within the grazing system. We found that within the grazing system, elk groups preferentially selected for rested pastures over pastures that were grazed during the previous spring (1 May–1 June), summer (1 June–15 July), and fall (15 September–30 September). The strength of selection against the pasture grazed during the summer growing season was strongest, and pastures grazed during the spring and fall were selected for over the pasture grazed during the summer. The number of elk utilizing the grazing system increased in the 19 yr following implementation of the grazing system; however, total elk herd size also increased during this time. We found no evidence that the proportion of the elk herd utilizing the grazing system changed following implementation of the rest–rotation grazing system. Wintering elk group preference for rested pastures suggests rested pastures play an important role in rotation grazing systems by conserving forage for wintering elk. Additionally, rested pastures provide important cover for a host of other wildlife species. We recommend wildlife managers maintain rested pastures within rotation grazing systems existing on ungulate winter range.