全文获取类型
收费全文 | 1461篇 |
免费 | 129篇 |
专业分类
林业 | 102篇 |
农学 | 31篇 |
基础科学 | 13篇 |
470篇 | |
综合类 | 44篇 |
农作物 | 60篇 |
水产渔业 | 182篇 |
畜牧兽医 | 545篇 |
园艺 | 24篇 |
植物保护 | 119篇 |
出版年
2024年 | 2篇 |
2023年 | 10篇 |
2022年 | 13篇 |
2021年 | 22篇 |
2020年 | 15篇 |
2019年 | 32篇 |
2018年 | 73篇 |
2017年 | 67篇 |
2016年 | 61篇 |
2015年 | 48篇 |
2014年 | 65篇 |
2013年 | 106篇 |
2012年 | 119篇 |
2011年 | 114篇 |
2010年 | 70篇 |
2009年 | 56篇 |
2008年 | 69篇 |
2007年 | 93篇 |
2006年 | 75篇 |
2005年 | 79篇 |
2004年 | 67篇 |
2003年 | 68篇 |
2002年 | 56篇 |
2001年 | 51篇 |
2000年 | 50篇 |
1999年 | 40篇 |
1998年 | 7篇 |
1997年 | 8篇 |
1996年 | 6篇 |
1995年 | 2篇 |
1994年 | 4篇 |
1992年 | 5篇 |
1991年 | 3篇 |
1990年 | 3篇 |
1989年 | 2篇 |
1988年 | 5篇 |
1987年 | 4篇 |
1986年 | 7篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1983年 | 3篇 |
1979年 | 2篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1973年 | 1篇 |
1972年 | 1篇 |
1945年 | 1篇 |
排序方式: 共有1590条查询结果,搜索用时 15 毫秒
991.
Chitimia L Muñoz-García CI Sánchez-Velasco D Lizana V Del Río L Murcia L Fisa R Riera C Giménez-Font P Jiménez-Montalbán P Martínez-Ramírez A Meseguer-Meseguer JM García-Bacete I Sánchez-Isarria MA Sanchis-Monsonís G García-Martínez JD Vicente V Segovia M Berriatua E 《Veterinary parasitology》2011,181(1):12-16
An epidemiological study was carried out to investigate asymptomatic Leishmania infantum infection by PCR and ELISA in wild rabbits, humans and domestic dogs in southeastern Spain. Seroprevalence was 0% (0/36) in rabbits, 2% (13/657) in humans and 7% (14/208) in dogs. The prevalence of PCR-positives was 0.6% (1/162) in rabbits tested in a wide range of tissue samples, 2% (8/392) in humans analysed in blood samples and 10% (20/193) and 67% (29/43) in dogs analysed in blood and lymphoid tissue samples, respectively. Results suggest that wild rabbits have a very low risk of becoming chronically infected with L. infantum, and provide further evidence that cryptic L. infantum infection is widespread in the domestic dog population and is also present in a comparatively smaller proportion of healthy humans. The epidemiological and clinical implications of these findings are discussed. 相似文献
992.
Rossini M García G Rojas J Zerpa H 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2011,40(3):374-379
Background: Commercial farming of Caiman crocodilus crocodilus has had an impact on the use of this species for meat consumption and the leather industry. Spectacled Caimans comprise part of the South American plains biodiversity. Misinterpretation of laboratory data is a risk owing to the limited hematologic and serum biochemical values available for this species. Objective: The aim of this study was to determine hematologic and serum biochemical values for wild Spectacled Caimans from the Venezuelan plains. Methods: Blood samples were collected form the caudal tail vein of 100 Spectacled Caimans (40 males and 60 females) from the plains located in the State of Apure. Values for RBC count, PCV, hemoglobin concentration, WBC absolute and differential counts, and thrombocyte counts were obtained using manual methods, and RBC indices were calculated. Serum biochemical analysis included measurement of alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, and creatine kinase activities and concentrations of total protein and albumin. Comparisons between sexes were analyzed using the Mann–Whitney test. Results: Reference values for wild Spectacled Caimans were determined. Minor differences in hematologic values, particularly for RBC counts, were found compared with previously published values for this species. Serum biochemical values were similar to those available for other crocodilians. There were no significant differences between males and females. Conclusions: Minor differences between the values obtained for wild Spectacled Caimans and those previously published for this species may be related to differences in methodology and environmental conditions. Availability of hematologic and serum biochemical reference values will be useful for accurate diagnosis and management of disease in this species. 相似文献
993.
Simon-Grifé M Martín-Valls GE Vilar MJ García-Bocanegra I Mora M Martín M Mateu E Casal J 《Veterinary microbiology》2011,149(1-2):56-63
Swine influenza is caused by type A influenza virus. Pigs can be infected by both avian and human influenza viruses; therefore, the influenza virus infection in pigs is considered an important public health concern. The aims of present study were to asses the seroprevalence of swine influenza subtypes in Spain and explore the risk factors associated with the spread of those infections. Serum samples from 2151 pigs of 98 randomly selected farms were analyzed by an indirect ELISA for detection of antibodies against nucleoprotein A of influenza viruses and by the hemagglutination inhibition (HI) using H1N1, H1N2 and H3N2 swine influenza viruses (SIV) as antigens. Data gathered in questionnaires filled for each farm were used to explore risk factors associated with swine influenza. For that purpose, data were analyzed using the generalized estimating equations method and, in parallel by means of a logistic regression. By ELISA, 92 farms (93.9%; CI(95%): 89.1-98.7%) had at least one positive animal and, in total, 1340/2151 animals (62.3%; CI(95%): 60.2-64.3%) were seropositive. A total of 1622 animals (75.4%; CI(95%): 73.6-77.2%) were positive in at least one of the HI tests. Of the 98 farms, 91 (92.9%; CI(95%): 87.7-98.1%) had H1N1 seropositive animals; 63 (64.3%; CI(95%): 54.6-73.9%) had H1N2 seropositive pigs and 91 (92.9%; CI(95%): 87.7-98.1%) were positive to H3N2. Mixed infections were detected in 88 farms (89.8; CI(95%): 83.7-95.9%). Three risk factors were associated with seroprevalences of SIV: increased replacement rates in pregnancy units and, for fatteners, existence of open partitions between pens and uncontrolled entrance to the farm. 相似文献
994.
Vilar Saavedra P Lara García A Zaldívar López S Couto G 《Veterinary journal (London, England : 1997)》2011,190(2):e78-e83
Hemostatic abnormalities were investigated in 32 dogs with carcinoma and 19 age-matched healthy dogs. Thromboelastography, hemostasis profile (i.e. prothrombin time [PT], activated partial thromboplastin time [aPTT], fibrinogen concentration), platelet count (PLT), thrombin-antithrombin complexes (TAT), and plasminogen activator inhibitor-1 (PAI-1) activity were evaluated. Dogs with carcinomas had faster thrombus generation (TEG(TG), a mathematic value obtained from the first derivate of the thromboelastographic tracing; 834.8±91.1 vs. 707.8±75.8mm/min; mean±SD), increased fibrinogen concentration (276 vs. 151mg/dL), and PLT (425 vs. 324U×10(9)/L), but had decreased PAI-1 activity (15.7 vs. 26.2IU/mL).The most common hemostatic abnormalities found in carcinoma dogs were hypercoagulability (TEG(TG)>mean+2 SD of healthy dogs) and thrombocytosis (PLT>424×10(9)U/L) in 46% of cases, and hyperfibrinogenemia (fibrinogen >384mg/dL) in 32% of cases. Disseminated intravascular coagulation was uncommon and the extent of disease was not correlated with hypercoagulability. TEG(TG) showed good correlation with fibrinogen (r=0.80) and hyperfibrinogenemia seems to be a main factor of the hypercoagulable state in carcinoma dogs. In conclusion, TEG(TG) is a valid parameter to diagnose hypercoagulability. 相似文献
995.
Manuel López-Vicente Noemí Lana-Renault José Maria García-Ruiz Ana Navas 《Journal of Soils and Sediments》2011,11(8):1440-1455
Purpose
Sediment delivery from headwater catchments to reservoirs is a serious threat to reservoir sustainability and is a critical issue in Mediterranean environments where water resources are scarce. In this study we assessed the consequences of two landscape management scenarios (natural vegetation recovery and scrub clearance) on soil erosion and sediment yield. The results were analyzed in relation to predicted and measured rates of soil erosion and sediment yield, with the aim of promoting better management practices. 相似文献996.
Scagnoli V Staub U Bodenthin Y de Souza RA García-Fernández M Garganourakis M Boothroyd AT Prabhakaran D Lovesey SW 《Science (New York, N.Y.)》2011,332(6030):696-698
Orbital currents are proposed to be the order parameter of the pseudo-gap phase of cuprate high-temperature superconductors. We used resonant x-ray diffraction to observe orbital currents in a copper-oxygen plaquette, the basic building block of cuprate superconductors. The confirmation of the existence of orbital currents is an important step toward the understanding of the cuprates as well as materials lacking inversion symmetry, such as magnetically induced multiferroics. Although observed in the antiferromagnetic state of cupric oxide, we show that orbital currents can occur even in the absence of long-range magnetic moment ordering. 相似文献
997.
998.
Candresse T Saenz P García JA Boscia D Navratil M Gorris MT Cambra M 《Phytopathology》2011,101(5):611-619
Typing of the particular Plum pox virus (PPV) strain responsible in an outbreak has important practical implications and is frequently performed using strain-specific monoclonal antibodies (MAbs). Analysis in Western blots of the reactivity of 24 MAbs to a 112-amino-acid N-terminal fragment of the PPV coat protein (CP) expressed in Escherichia coli showed that 21 of the 24 MAbs recognized linear or denaturation-insensitive epitopes. A series of eight C-truncated CP fragments allowed the mapping of the epitopes recognized by the MAbs. In all, 14 of them reacted to the N-terminal hypervariable region, defining a minimum of six epitopes, while 7 reacted to the beginning of the core region, defining a minimum of three epitopes. Sequence comparisons allowed the more precise positioning of regions recognized by several MAbs, including those recognized by the 5B-IVIA universal MAb (amino acids 94 to 100) and by the 4DG5 and 4DG11 D serogroup-specific MAbs (amino acids 43 to 64). A similar approach coupled with infectious cDNA clone mutagenesis showed that a V74T mutation in the N-terminus of the CP abolished the binding of the M serogroup-specific AL MAb. Taken together, these results provide a detailed positioning of the epitopes recognized by the most widely used PPV detection and typing MAbs. 相似文献
999.
Among various derivatives of tetramethyl-diamino-diphenylmethane only the quinoid-structured dye salts had bacteriostatic activity in our experiments, while the leucoderivatives were inactive. 相似文献
1000.
Sage C Huang M Karimi K Gutierrez G Vollrath MA Zhang DS García-Añoveros J Hinds PW Corwin JT Corey DP Chen ZY 《Science (New York, N.Y.)》2005,307(5712):1114-1118
In mammals, hair cell loss causes irreversible hearing and balance impairment because hair cells are terminally differentiated and do not regenerate spontaneously. By profiling gene expression in developing mouse vestibular organs, we identified the retinoblastoma protein (pRb) as a candidate regulator of cell cycle exit in hair cells. Differentiated and functional mouse hair cells with a targeted deletion of Rb1 undergo mitosis, divide, and cycle, yet continue to become highly differentiated and functional. Moreover, acute loss of Rb1 in postnatal hair cells caused cell cycle reentry. Manipulation of the pRb pathway may ultimately lead to mammalian hair cell regeneration. 相似文献