Molecular epidemiology of Newcastle disease viruses in Vietnam

Newcastle disease virus (NDV) causes significant economic losses to the poultry industry in Southeast Asia. In the present study, 12 field isolates of NDV were recovered from dead village chickens in Vietnam between 2007 and 2012, and were characterized. All the field isolates were classified as velogenic. Based on the sequence analysis of the F variable region, two distinct genetic groups (Vietnam genetic groups G1 and G2) were recognized. Phylogenetic analysis revealed that all the 12 field isolates fell into the class II genotype VII cluster. Ten of the field isolates, classified as Vietnam genetic group G1, were closely related to VIIh viruses that had been isolated from Indonesia, Malaysia, and Cambodia since the mid-2000s, while the other two field isolates, of Vietnam genetic group G2, clustered with VIId viruses, which were predominantly circulating in China and Far East Asia. Our results indicate that genotype VII viruses, especially VIIh viruses, are predominantly responsible for the recent epizootic of the disease in Vietnam.     

宁夏引黄灌区冬小麦旱追肥肥效实验

在宁夏引黄灌区成功引种冬小麦,可促进产业结构调整,建立更高效的种植制度;但北方春季干旱多风、气温低与冬小麦返青期早、幼穗分化期遭遇缺水缺肥环境的矛盾有待进一步解决.探讨利用冬小麦返青前后灌区土壤返潮进行机械旱追肥的可行性及其增产效果,结果表明:冬小麦返青后至头次灌水前若没有自然降雨,机械旱追肥无增产效果,且有负面效应.原因主要是氮肥在土壤中易形成高浓度溶液,造成烧根烧苗,不利于促蘖增穗;在灌区干旱条件下,氮肥施用应结合灌溉水进行,才可充分发挥其增产效果.因此冬小麦返青至头水期间,田间管理应在潮水退前及早管理,提水保墒,有条件的区域早灌头水.机械旱追肥与种植前茬、土壤湿度、降雨、种植品种分蘖特性、苗情等关系较大,应综合考虑.     

Growth performance,carcass quality characteristics and colonic microbiota profiles in finishing pigs fed diets with different inclusion levels of rice distillers’ by‐product

The aim of this study was to evaluate the effects of diets containing rice distillers’ by‐product (RDP) on growth performance, carcass characteristics, meat quality, and gut microbiota of fattening pigs. Twenty‐four crossbred finishing pigs (Duroc × Landrace × Yorkshire), 56.9 ± 3.1 kg initial body weight, were randomly allocated to three groups. For 56 days, pigs were fed one of three diets including RDP0 (control), RDP15 (15% RDP in DM), and RDP30 (30% RDP in DM). With RDP level in diet, average daily gain and backfat thickness linearly increased (p < 0.05), and drip loss tended to increase (p ≤ 0.08). In addition, 16S ribosomal RNA gene amplicon profiling showed that RDP was associated with modulation of colonic microbiota composition, especially at family and genus levels. Relative abundance of Porphyromonadaceae and Erysipelotrichaceae families in colonic digesta increased with inclusion of RDP, while that of Enterobacteriaceae decreased. The proportion of genera unclassified Erysipelotrichaceae, and Butyrivibrio increased as inclusion of RDP. These results indicate that up to 30% inclusion in diet of finishing pigs, RDP can modulate colonic microbiota composition, and induces an improvement of animal growth and fat deposition.     

云贵川三省部分地区警犬狂犬病血清中和抗体调查

为评价警犬的狂犬病免疫效果,了解影响免疫效果的因素,2017年在云南、贵州、四川三省部分地区免疫狂犬病疫苗的警犬中随机抽样,采用荧光抗体病毒中和试验(FAVN),检测血清中狂犬病病毒中和抗体效价。结果显示,共抽检187只警犬,中和抗体阳性127只(≥0.50 IU/m L),总体免疫合格率为67.91%,其中云南、贵州、四川3省的免疫合格率分别为57.14%、68.33%、76.06%。结果分析表明,警犬狂犬病免疫合格率与犬的年龄、免疫次数、疫苗类型等因素有关,而与性别和品种无关。建议完善警犬免疫程序,加大血清中和抗体监测力度,对不合格警犬及时加强免疫;对每个影响因素进行连续性分析,以确定影响警犬狂犬病免疫合格率的具体因素。     

A soil temperature control system for ecological research in greenhouses

We designed and tested a soil temperature control system for plant ecophysiological experiments in greenhouses and growth chambers. The system consists of a plywood box, polyethylene liner, insulation, seedling containers, a water pump, and a flow-through heater or chiller. One hundred and twelve seedling containers (11cm diameter, 13.5 cm high) are mounted in the plywood box. There is a hole at the bottom center of each container to allow the free drainage of irrigation water and fertilizer solution. The space between containers is filled with water that is circulated through the chiller/heater. The water is also circulated within the plywood box by a water pump to increase the uniformity of temperature. The system was tested for three soil temperatures (5, 20, and 30°C) over a period of four months. The containers were filled with a peat-moss vermiculite mixture and planted with tree seedlings. The test showed that the soil temperature was almost equal to the water temperature for all three soil temperatures (regression slop = 0.99, intercept = 0.12,r ² = 1.00). The average soil temperatures were within (0.41°C of the set values. The soil temperature of the 112 containers within the same box followed a normal distribution with a small standard deviation (0.34°C for the 30°C treatment). There was a temperature gradient from the top to the bottom of the container (< 1°C). The direction of the temperature gradient was determined by the direction of temperature difference between the soil and the ambient air. When the soil temperature was lower than air temperature, the soil temperature decreased from the top to the bottom of the container, and vise versa. The soil temperature was higher during the day than at night (difference < 1.5°C). Funding for this research was provided by Lakehead University, CFI, ORDCF, and NSERC research grants to Dang, and an NSERC PGS A to Cheng.     

棉花叶螨的发生及其综合防治技术措施

该文阐述了棉叶螨形态、危害症状、发生规律、传播途径和因地制宜地采取农业、化学、生物等多种手段进行综合防治技术措施。     

澳大利亚动物疫病防控体系简介

澳大利亚动物疫病净化工作有许多值得借鉴之处。本文就澳大利亚畜牧业基本情况、兽医服务体系、动物标识和疫病追溯体系和动物疫病应急管理体系等方面进行了介绍,列举了7个方面的可借鉴之处,包括联邦政府和各州政府动物疫病防控分工明确、各级兽医服务专业化程度高、行业协会在动物疫病控制中地位显著、动物标识体系完善、动物疫病控制规划详尽、疫病净化政策坚持不懈和兽医流行病学技术应用纯熟等,以期对我国动物疫病防控体系建设工作有所启发。     

创建无疫小区的现实意义与关键措施

本文阐述了我国现阶段创建无疫小区的背景、优势和意义,结合10年来指导企业创建无疫小区的工作实践,指出基层兽医管理部门和企业在创建无疫小区过程中存在的主要问题,并针对性提出了9条措施,以期对今后无疫小区创建实践提供参考.     

Effects of polyethylene glycol and a synthetic ice blocker during vitrification of immature porcine oocytes on survival and subsequent embryo development

We evaluated the effects of polyethylene glycol (PEG) and Supercool X‐1000 (SC) as supplements during the vitrification of immature cumulus‐enclosed porcine oocytes in a solution based on 17.5% ethylene glycol + 17.5% propylene glycol. After warming, the oocytes were subjected to in vitro maturation, fertilization and embryo culture. In Experiment 1, equilibration and vitrification solutions were supplemented with or without 2% (w/v) PEG (PEG+ and PEG‐, respectively). The survival rate, cleavage and blastocyst development were similar between PEG+ and PEG‐ groups; however, all values were lower than those in the non‐vitrified control. In Experiment 2, vitrification solution was supplemented with or without 1% (v/v) SC (SC+ and SC‐, respectively). The percentages of survival and blastocyst development were similar between SC+ and SC‐ groups but lower than those in the non‐vitrified control. The percentage of cleavage in the SC‐ group was significantly lower than the control and the SC+ groups, which were in turn similar to one another. In both experiments, the cell numbers in blastocysts were not significantly different among the non‐vitrified and vitrified groups. In conclusion, PEG did not improve oocyte survival and embryo development, whereas SC improved the ability of surviving oocytes to cleave but not to develop into blastocysts.