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【目的】旨在寻找与产羔数相关的遗传标记,为绵羊高繁殖力的标记辅助选择提供科学依据。【方法】以控制Lacaune绵羊高繁殖力的骨形态发生蛋白15(bonemorphogenetic protein15,BMP15)基因为候选基因,采用PCR-SSCP方法在绵羊(Ovisaries)高繁殖力品种(小尾寒羊和湖羊)和低繁殖力品种(多赛特、特克塞尔、考力代、杜泊、南非肉用美利奴和中国美利奴绵羊)中检测BMP15基因的FecXL突变,同时研究该基因突变对小尾寒羊和湖羊高繁殖力的影响。【结果】这8个绵羊品种都没有发生与Lacaune绵羊相同的FecXL突变(C53Y)。【结论】BMP15基因中影响Lacaune绵羊高繁殖力的突变位点对小尾寒羊和湖羊的高繁殖力都没有显著影响。 相似文献
123.
用绵羊进行肺炎病毒接种4只山羊3-4个月后,可观察到接毒山羊都出现了发育迟缓和消瘦现象,并有1只山羊山现了明显的临床病症。而未接毒的对照山羊则发育正常。接毒28d后,可以从接毒山羊的外周血单核细胞中分离到病毒。病理剖检发现4只接毒山羊中有1只山羊的多种器官出现了较为严重的病变,组织学检查则可看到典型的间质性肺炎、中度的脑炎和较为严重的脾炎的症变。以上的结果表明OPPV可以感染山羊,并对山羊有较强的 相似文献
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126.
油樟油细胞和粘液细胞发育的超微结构 总被引:1,自引:0,他引:1
利用超薄切片法和透射电镜研究油樟油细胞和粘液细胞发育过程。依据油细胞3层细胞壁的发育将其分为4个阶段。阶段1:仅有初生纤维素壁层,又可分为原始细胞和细胞液泡化二时期。质体内具白色小泡和黑色嗜饿滴,细胞质中有黑色或灰色的嗜饿物质,以及嗜饿物质与液泡的融合。阶段2:栓质化壁层的形成。片层状的栓质叠加在初生纤维素壁内侧。阶段3:内纤维素壁层的形成。较厚而结构松散的内纤维素壁层逐步形成,并叠加在栓质化壁层的内侧,大液泡成为充满嗜饿油脂的油囊。阶段4:油细胞成熟及细胞质解体。杯形构造由内纤维素壁层向细胞腔内突起形成,油囊由液泡包被连接到杯形构造中。解体的细胞质变得电子不透明或呈杂乱状态。粘液细胞发育方式有两种:一种是由内纤维素形成以后的油细胞发育而来,其细胞质中不断产生以同心圆或螺旋线方式排列的多膜结构,并充满整个细胞腔,最后多膜结构解体而成为丝状或颗粒状的粘液;另一种是由已完全成熟的油细胞发育而来,其油囊中的油呈不均匀的状态,并产生局部降解点,逐步扩大,最后油完全降解成颗粒成或丝状的粘液。 相似文献
127.
Methylene chloride and methanol extracts of 20 Indonesian plants with ethnomedical uses have been assessed for in vitro antibacterial and antifungal properties by disk diffusion method. Extracts of the six plants: Terminalia catappa, Swietenia mahagoni Jacq., Phyllanthus acuminatus, Ipomoea spp., Tylophora asthmatica and Hyptis brevipes demonstrated high activity in this bioassay system. These findings should stimulate the search for novel, natural product such as new antibacterial and antifungal agents. 相似文献
128.
From January 1999 to May 2001, we investigated seasonal variations in the photosynthetic capacity of Taiwan spruce (Picea morrisonicola Hay.) growing in the subalpine region of subtropical Taiwan (23 degrees 29' N, 120 degrees 53' E, 2600 m a.s.l.). Photosynthetic capacity (near light-saturated net photosynthetic rate, Pnsat, chlorophyll fluorescence (Fv/Fm) and soluble protein concentration of needles all increased from mid or late spring to early winter. Even when minimum air temperature of the measuring day dropped to near 0 degrees C, Pnsat remained at about 20% of the highest value observed in winter. There was a curvilinear relationship between Fv/Fm and the minimum or mean air temperature of the measuring day. The increase in Fv/Fm with temperature was slowed when the daily mean air temperature was above 7 degrees C, or the minimum air temperature was above 3 degrees C; however, when air temperatures dropped below these values, Fv/Fm varied sharply. Seasonal variations in Pnsat paralleled those in Fv/Fm and needle soluble protein concentration. In early or mid spring when air temperature and Fv/Fm increased, Pnsat and soluble protein concentration remained low. Multiple regression analysis showed that seasonal variations in Pnsat were affected by Fv/Fm, air temperature and needle soluble protein concentration, and the multiple regression equation could be used to estimate Pnsat in different seasons. We conclude that the decrease in photosynthetic capacity of Taiwan spruce in winter and its subsequent recovery in spring were mainly caused by photoinhibition and its reversal, and changes in needle soluble protein concentration. Another possible explanation for the delayed recovery of photosynthetic capacity in spring may be associated with the slow increase in needle soluble protein concentration. 相似文献
129.
上海新建绿地的土壤质量现状和对策 总被引:4,自引:0,他引:4
对上海新建的18块绿地319个土壤样品的调查分析显示,上海新建城市绿地土壤质量存在以下一些共性:pH呈碱性或强碱性;电导率(EC值)和有机质含量偏低;土壤密度偏高,42.45%的土壤大于1.35Mg·m^-3;土壤通气性差,83.2%的土壤通气孔隙度〈5%;土壤阳离子含量适中,其中水解性氮和有效磷含量低,但速效钾含量适中;土壤质地基本为壤质土。土壤质量是限制上海新建城市绿地植物生长和生态景观效果发挥的主要限制因子之一,针对上海城市土壤存在问题,建议从城市土壤标准建立、采样方法、测试方法、植物营养平衡、土壤安全性、土壤改良与废弃物利用、管理对策等几个方面提高城市绿地的土壤质量。 相似文献
130.
Sheng-Yang Wang Wan-Chi Lai Fang-Hua Chu Chien-Tsong Lin Shi-Yen Shen Shang-Tzen Chang 《Journal of Wood Science》2006,52(6):522-526
This is the first article to report the evaluation of a natural product used as an antisilverfish agent. Silverfish (Lepisma saccharina), primitive wingless insects, feed on a variety of materials, including paper, cotton, starch, and cereals. They can be a
problem in libraries and other places where books, documents, and papers are stored. In this pilot study, the essential oil
from leaves of Cryptomeria japonica was investigated to test its properties as a silverfish repellent and insecticide. The results from a repellency bioassay
show that the essential oil significantly repelled silverfish. The repellent activity was 80% at a dosage of 0.01 mg/cm3. When silverfish were exposed to a concentration of 0.16 mg/cm3 of essential oil, they were killed within 10h. The chemical composition of essential oil, the emissions from a test chamber,
and the residue left on filter papers previously soaked with the essential oil in a chamber were analyzed by gas chromatography-mass
spectrometry. The components of the essential oil were found to be: elemol (18.22%), 16-kaurene (11.63%), 3-carene (9.66%),
sabinene (9.37%), 4-terpineol (9.06%), β-eudesmol (5.70%), α-pinene (5.62%), and limonene (5.26%). Only some constituents of the essential oil compounds collected by solid-phase microextraction
were found to be emitted in the test chamber. The main constituents were: 3-carene (21.03%), p-cymene (10.95%), limonene (9.49%), β-myrcene (9.39%), γ-terpinene (9.10%), α-terpinene (8.57%), and 4-terpineol (7.97%). 相似文献