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991.
Hurtado-Fernández E Carrasco-Pancorbo A Fernández-Gutiérrez A 《Journal of agricultural and food chemistry》2011,59(6):2255-2267
A powerful HPLC-DAD-ESI-TOF MS method was established for the efficient identification of the chemical constituents in the methanolic extracts of avocado (Persea americana). Separation and detection conditions were optimized by using a standard mix containing 39 compounds belonging to phenolic acids and different categories of flavonoids, analytes that could be potentially present in the avocado extracts. Optimum LC separation was achieved on a Zorbax Eclipse Plus C18 analytical column (4.6×150 mm, 1.8 μm particle size) by gradient elution with water+acetic acid (0.5%) and acetonitrile as mobile phases, at a flow rate of 1.6 mL/min. The detection was carried out by ultraviolet-visible absorption and ESI-TOF MS. The developed method was applied to the study of 3 different varieties of avocado, and 17 compounds were unequivocally identified with standards. Moreover, around 25 analytes were tentatively identified by taking into account the accuracy and isotopic information provided by TOF MS. 相似文献
992.
Barata A Campo E Malfeito-Ferreira M Loureiro V Cacho J Ferreira V 《Journal of agricultural and food chemistry》2011,59(6):2543-2553
In the present work, the aroma profiles of wines elaborated from sound and sour rot-infected grapes as raw material have been studied by sensory analysis, gas chromatography-olfactometry (GC-O), and gas chromatography-mass spectrometry (GC-MS), with the aim of determining the odor volatiles most likely associated with this disease. The effect of sour rot was tested in monovarietal wines produced with the Portuguese red grape variety Trincadeira and in blends of Cabernet Sauvignon and sour rotten Trincadeira grapes. Wines produced from damaged berries exhibited clear honey-like notes not evoked by healthy samples. Ethyl phenylacetate (EPhA) and phenylacetic acid (PAA), both exhibiting sweet honey-like aromas, emerged as key aroma compounds of sour rotten wines. Their levels were 1 order of magnitude above those found in controls and reached 304 and 1668 μg L(-1) of EPhA and PAA, respectively, well above the corresponding odor thresholds. Levels of γ-nonalactone also increased by a factor 3 in sour rot samples. Results also suggest that sour rot exerts a great effect on the secondary metabolism of yeast, decreasing the levels of volatiles related to fatty acids and amino acid synthesis. The highest levels of γ-decalactone of up to 405 μg L(-1) were also found in all of the samples, suggesting that this could be a relevant aroma compound in Trincadeira wine aroma. 相似文献
993.
J. Jesús Casas Juan S. Sánchez-Oliver Ana Sanz Miriam Furné Cristina Trenzado Melchor Juan Mariano Paracuellos María D. Suárez Francisca Fuentes Irene Gallego Carlos Gil José J. Ramos-Miras 《Biological conservation》2011,(1):253-262
We studied the relative value of natural habitats, river and coastal wetlands, and artificial habitats, irrigation canal and ponds, for the conservation of an endangered fish, Iberian toothcarp, in its southernmost area of distribution, characterised by agricultural intensification. Our results show that the bulk of the population of the Iberian toothcarp is concentrated in irrigation ponds. Natural habitats sustained null or impoverished subpopulations, and individuals showed signs of low metabolic activity. This coincided with the relatively high habitat quality observed in ponds, particularly those with submerged aquatic vegetation, in contrast with the chronic eutrophication of the coastal lagoons. In spite of a generalised aggressive management in the irrigation system, featured by periodic vegetation clearance, desiccation and biocide treatment, the subpopulation of the Iberian toothcarp thrives in it probably thanks to adequate water quality and to an active path dynamics maintained by connectivity through the canal. Agro-environmental measures are discussed for the improvement of this species conservation in natural and artificial habitats. 相似文献
994.
Luis V. García Cristina Ramo Cristina Aponte Adela Moreno María T. Domínguez Lorena Gómez-Aparicio Ramón Redondo Teodoro Marañón 《Biological conservation》2011,(2):764-771
Conservation management conflicts frequently arise when an overpopulation of a protected organism has negative effects on other valuable elements in the same ecosystem. We studied the interactions between a colony of protected tree-nesting wading birds and a remnant population of centenarian cork oaks that was part of the formerly dominant forests in the Doñana Biological Reserve (SW Spain). A significant increase in the tree mortality rates has been recorded in areas that are yearly influenced by the bird colony.We analysed a cohort of surviving trees using a gradient of nesting bird influence. Tree-nesting history, bird isotopic signature (δ15N), tree health-related parameters (defoliation, δ13C and leaf surface coverage by faeces) and several soil variables were evaluated. Bird influence was related to increased soil salinity. This increase correlated to increased water-use efficiency for the leaves and to crown defoliation, suggesting that the heavily occupied trees are under higher stress and in poorer health condition than the unoccupied ones. We tested structural equations models (SEM) that were based on hypothesised bird effects on the health of the trees. Soil-mediated effects of the nesting birds best explained the symptoms of the declining health of the trees, whereas the percent of leaves’ surface that was covered by faeces did not improve the fitted SEM model.For the reserve’s managers, a challenging trade-off exists between preserving the relict trees, which have a high genetic diversity and a key ecological role in these savannah-like ecosystems, and maintaining the current nesting area for these protected, but expanding, wading birds. 相似文献
995.
Microbial community composition and carbon cycling within soil microenvironments of conventional, low-input, and organic cropping systems 总被引:1,自引:0,他引:1
This study coupled stable isotope probing with phospholipid fatty acid analysis (13C-PLFA) to describe the role of microbial community composition in the short-term processing (i.e., C incorporation into microbial biomass and/or deposition or respiration of C) of root- versus residue-C and, ultimately, in long-term C sequestration in conventional (annual synthetic fertilizer applications), low-input (synthetic fertilizer and cover crop applied in alternating years), and organic (annual composted manure and cover crop additions) maize-tomato (Zea mays - Lycopersicum esculentum) cropping systems. During the maize growing season, we traced 13C-labeled hairy vetch (Vicia dasycarpa) roots and residues into PLFAs extracted from soil microaggregates (53-250 μm) and silt-and-clay (<53 μm) particles. Total PLFA biomass was greatest in the organic (41.4 nmol g−1 soil) and similar between the conventional and low-input systems (31.0 and 30.1 nmol g−1 soil, respectively), with Gram-positive bacterial PLFA dominating the microbial communities in all systems. Although total PLFA-C derived from roots was over four times greater than from residues, relative distributions (mol%) of root- and residue-derived C into the microbial communities were not different among the three cropping systems. Additionally, neither the PLFA profiles nor the amount of root- and residue-C incorporation into the PLFAs of the microaggregates were consistently different when compared with the silt-and-clay particles. More fungal PLFA-C was measured, however, in microaggregates compared with silt-and-clay. The lack of differences between the mol% within the microbial communities of the cropping systems and between the PLFA-C in the microaggregates and the silt-and-clay may have been due to (i) insufficient differences in quality between roots and residues and/or (ii) the high N availability in these N-fertilized cropping systems that augmented the abilities of the microbial communities to process a wide range of substrate qualities. The main implications of this study are that (i) the greater short-term microbial processing of root- than residue-C can be a mechanistic explanation for the higher relative retention of root- over residue-C, but microbial community composition did not influence long-term C sequestration trends in the three cropping systems and (ii) in spite of the similarity between the microbial community profiles of the microaggregates and the silt-and-clay, more C was processed in the microaggregates by fungi, suggesting that the microaggregate is a relatively unique microenvironment for fungal activity. 相似文献
996.
Robert Brus Dalibor Ballian Peter Zhelev Marija Pandža Martin Bobinac Jane Acevski Yannis Raftoyannis Kristjan Jarni 《European Journal of Forest Research》2011,130(4):657-670
We examined leaf and mature seed cone variation of Juniperus oxycedrus L. subsp. oxycedrus in 12 natural populations across the species range in the Balkan Peninsula. We measured 10 morphological traits from a minimum
of 100 leaves in each of 190 individuals, and two morphological traits from 30–50 seed cones in each of 94 females. High phenotypic
variation was found, but no geographical structure or cline across populations was detected for any of the studied traits.
Mean values of comparable leaf and cone morphological traits did not differ considerably from values reported elsewhere. Gender
dimorphism in leaf morphology was detected, but it was not distributed uniformly throughout the studied area. An ANOVA model
with both nested and crossed effects revealed that the largest proportion of the total variation was, as expected, contained
within populations, partly as among-tree variation (18–47%, depending on the trait) and partly as within-tree variation (33–77%),
which was remarkably high. Gender dimorphism explained only 0–3% of the total variation. Differences among populations (2–23%)
were significant for all studied traits except one; however, PCA showed no clear geographical differentiation of the studied
populations. This lack of phylogeographical structure may be the consequence of repeatedly occurring colonisation-retreat
scenarios and suggests the existence of several small refugial populations scattered over a large part of the Balkan Peninsula
in the Pleistocene. Further research including palaeobotanical and molecular genetic studies will be needed to better understand
the forces that shaped current variation patterns of J. oxycedrus L. subsp. oxycedrus in the Balkan Peninsula. 相似文献
997.
Serena Santolamazza-Carbone Montserrat Pestaña José Antonio Vega 《Journal of pest science》2011,84(3):343-353
The spectrum and dynamics of xylophagous insects infesting Pinus pinaster after a fire event were studied from November 2007 to December 2008 at Sierra de Outes (Galicia, NW Spain). Insects were
collected within and outside the fire area. Relationships among species status, species abundance, tree parameters and tree
injury were assessed. Mortality of injured trees was also recorded. Insect colonization built up consistently during the first
year after the fire, although the pests did not colonize unburned trees in nearby areas. Thirteen insect species from six
xylophagous families were collected. Tomicus spp., Buprestis novenmaculata, Anobium punctatum and Pissodes castaneus were the first species detected. Tomicus spp. (29%) and Ips sexdentatus (23%) were the dominant taxa. Bole char height and soil burn severity were positively correlated with insect presence. Trees
with short stem diameter and thinner bark were also preferred. B. novenmaculata presence was positively correlated with crown scorch. Loss of cambium hydration was negatively related to the occurrence
of pests, likely because sapwood desiccation and loss of nutrient impede brood development. About 33.3% of the fire-scorched
pines died. The results recommend the removal of injured and dying pines after fire, to avoid the rise of pest population
threatening recovering trees in the burned areas. 相似文献
998.
M. R. Mosquera-Losada R. Cuiña-Cotarelo A. Rigueiro-Rodríguez 《European Journal of Forest Research》2011,130(6):997-1008
Tree–understory competition is one of the most important aspects that control tree growth after reforestation. The relationship
between trees and the understory can be modified by improving acidic soils with lime and by fertilisation. This experiment
aims to evaluate the effect of soil improvements on the pasture–tree relationship by liming and fertilisation on different
dates in a Pinus radiata-reforested area. Both lime and sewage sludge improved soil fertility by increasing Ca and reducing Al in the soil. Initially,
tree development was reduced by lime, which improved the establishment of competitive grasses. Tree growth in limed treatments
did not initially respond to sludge inputs, likely because both tree and grass roots shared the same soil depth layer. Three
years after establishment, the use of high doses of sewage sludge in limed plots caused a growth rate similar to the best
treatments of unlimed plots, which grew with a poorly sown grass establishment. After 2 years of the experiment, the presence
of Erica woody shrub diminished tree development. High doses of sewage sludge with lime, as well as high doses of sewage sludge without
lime, applied in April and low doses of sewage sludge without lime added in early February improved tree growth. From a practical
point of view, lime and sewage sludge dose close to 100 kg total N ha−1 should be recommended if a silvopastoral system is established, as it enhances pasture production and tree growth. 相似文献
999.
Lara Vilar del Hoyo M. Pilar Martín Isabel F. Javier Martínez Vega 《European Journal of Forest Research》2011,130(6):983-996
About 90% of the wildland fires occurred in Southern Europe are caused by human activities. In spite of these figures, the
human factor hardly ever appears in the definition of operational fire risk systems due to the difficulty of characterising
it. This paper describes two spatially explicit models that predict the probability of fire occurrence due to human causes
for their integration into a comprehensive fire risk–mapping methodology. A logistic regression technique at 1 × 1 km grid
resolution has been used to obtain these models in the region of Madrid, a highly populated area in the centre of Spain. Socio-economic
data were used as predictive variables to spatially represent anthropogenic factors related to fire risk. Historical fire
occurrence from 2000 to 2005 was used as the response variable. In order to analyse the effects of the spatial accuracy of
the response variable on the model performance (significant variables and classification accuracy), two different models were
defined. In the first model, fire ignition points (x, y coordinates) were used as response variable. This model was compared with another one (Kernel model) where the response variable
was the density of ignition points and was obtained through a kernel density interpolation technique from fire ignition points
randomly located within a 10 × 10 km grid, which is the standard spatial reference unit established by the Spanish Ministry
of Environment, Rural and Marine Affairs to report fire location in the national official statistics. Validation of both models
was accomplished using an independent set of fire ignition points (years 2006–2007). For the validation, we used the area
under the curve (AUC) obtained by a receiver-operating system. The first model performs slightly better with a value of AUC
of 0.70 as opposed to 0.67 for the Kernel model. Wildland–urban interface was selected by both models with high relative importance. 相似文献
1000.
Jaime A. Ocampo-Hernández Patricia Tamez-Guerra Samuel Pineda Fernando Tamayo-Mejía Ariel Guzmán-Franco José I. Figueroa de la Rosa Ana Mabel Martínez 《Journal of pest science》2011,84(4):471-477
Entomopathogenic fungi represent excellent candidates for biological insecticides. Among the many entomopathogenic fungi, Beauveria bassiana (Balsamo) Vuillemin (Hypocreales: Clavicipitaceae) is one of the most promising species. Here, we report the results of infection studies performed using four B. bassiana native isolates (Bb37, Bb38, Bb40, and Bb45) compared with the commercial product Mycotrol® (strain GHA) on adult Mexican bean beetle Epilachna varivestis Mulsant (Coleoptera: Coccinellidae), one of the most important pests of bean production in Mexico. First, single concentration (1 × 108 conidia mL?1) assays were carried out using all isolates applied using an immersion method. Examinations at 10 days after inoculation indicated that isolates Bb37, Bb38, and Bb40 significantly reduced adult survival, causing 60–75% mortality compared to the commercial strain GHA (33%) and control (29%). Second, using two selected isolates (Bb37 and Bb40), the mean lethal concentration values (LC50) were estimated for third instar larvae and adult E. varivestis. The LC50 values for Bb37 and Bb40 isolates decreased 5.26-fold and 3.19-fold, respectively, in third instars compared with adults. However, such difference for Bb40 isolate was not significant. Finally, an experiment, to compare the median survival time (MST) values between third instar and adult E. varivestis, was conducted using Bb37 and Bb40 at 1 × 109 conidia mL?1. MST values were significantly reduced against larvae (87 and 100 h) compared with adults (130 and 134 h) by Bb37 and Bb40, respectively. We conclude that native isolates of B. bassiana represent an important alternative for the control of E. varivestis. However, to demonstrate its effectiveness under field conditions, detailed studies are needed. 相似文献