复合PCR鉴定胸膜肺炎放线杆菌和副猪嗜血杆菌方法的建立及初步应用

在已经建立的胸膜肺炎放线杆菌、副猪嗜血杆菌的单项PCR诊断方法的基础上,通过对扩增条件和引物的筛选,成功地建立了APP、HPS复合PCR诊断方法,利用一次PCR反应,即可同时扩增APP的442 bp,和HIS的1090 bp的特异性片段,并应用于临床检测.该方法的建立对临床上进行这2种疾病的鉴别诊断和混合感染的检测都具有重要意义.     

Bone marrow mesenchymal stem cells are induced to differentiate into cardiomyocytes by hepatocyte growth factor in vitro

AIM: To explore a new method of hepatocyte growth factor (HGF) inducing bone marrow mesenchymal stem cells (MSC) to differentiate into cardiomyocytes. METHODS: Bone marrow MSC was cultured with DMEM media (10% fetal calf serum) 4-6 passages, and induced by HGF (10 μg/L) for 30 d. Automatical beating of the differentiated cells was observed daily with transverse microscopy, or under condition of 0.1% isoproterenol or cal-cium-deprived incubation. Specific cardiac myosin in the cells was indentified by immunochemistry. RESULTS: At 14-20 d of differentiation, bone marrow mesenchymal stem cells formed clones, in 10%-50% of which spontaneous beating cell-mass had come to continuously exist. Isoproterenol increased the beating rate and calcium-deprived media inhibited the beating. The cells were identified to be cardiomyocytes by expression of cardiac myosin heavy chain. CONCLUSION: HGF may induce bone marrow mesenchymal stem cells into cardiomyocytes with high efficiency, but the differentiating pathway of stem cells remains to be further studied.     

Osteological development and anomalies in larval stage of hatchery‐reared yellow catfish Pelteobagrus fulvidraco

Comprehensive knowledge of osteological development of fish not only provides means for understanding its functional development, but also allows early detection of skeletal anomalies. The present study was conducted to determine osteological development and occurrence of anomalies from the first to the 40th day after hatching (DAH) for yellow catfish Pelteobagrus fulvidraco, a commercially important species in China. Vertebral ontogeny started with formation of anterior neural arches at 3 DAH, and completed at 6 DAH. Vertebral centra started to develop at 4 DAH and ossification in all centra was visible at 15 DAH. Caudal fin appeared at 2 DAH and ossification was visible at 26 DAH. The onset of dorsal and anal fins appeared at 4 DAH and their ossifications were visible at 20 DAH. Pectoral fins were present before first feeding and formed entirely at 9 DAH, and their ossifications were visible at 21 DAH. Pelvic fins appeared at 9 DAH, and formed completely at 21 DAH. The ossification of pelvic fins was not finished until the end of the experiment (40 DAH). 24 types of skeletal anomalies were observed. About 20% of individuals showed at least one anomaly. Haemal vertebrae anomalies occurred at the highest frequency, followed by pre‐haemal, caudal vertebrae and anal fin anomalies, and caudal fin had the lowest anomalies frequency. For the first time, our study determined osteological development and anomalies incidence in larval yellow catfish, which help further investigations into rearing conditions leading to appearance of these anomalies to prevent their incidence.     

47个香菇主栽菌株的SCAR标记分子鉴别

利用前期研究开发的10个SCAR分子标记对47个香菇主栽菌株的基因组DNA进行扩增分析.基于SCAR标记在检测菌株中的存在与缺失差异,7个香菇菌株被首先鉴别出来,其余40个菌株被分成11个小组,其中一些形态特征极为相近的菌株,如L241与L241-4、闽丰1号与L12、Cr02与7402等被相互辨别.     

基于多元化经营视角下的我国农业上市公司成长性问题研究

农业上市公司的成长应该说是一个持续的研究热点,社会经济发展需要这个产业的发展,需要这个产业微观主体农业上市公司的壮大,而对于成长中的多元化经营给企业带来应有的额外收益,在目前企业经营中还没有明显显现。作为我国先进农业生产力旗帜代表的农业上市公司,目前普遍存在着相对数量小、规模普遍较小、科技程度不高,还有就是生产经营过程中的背农多元化转型等问题,笔者认为这是一个事物发展的必然和允许存在的偏离,关键是正视和解决这些问题,本文将对我国农业上市公司成长中的多元化问题作分析研究。     

细指长臂虾的幼体发育

1991年4月，从长江口采回细指长臂虾抱卵亲虾，至实验室进行幼体培育。孵化后的幼体，在水温22℃．盐度12、饵料充足的条件下，有规律的每2d蜕皮1次，经10个状幼体期，变态为仔虾。如在发育后期遇饵料不足等不良环境条件的影响，则幼体须经11次蜕皮后，始能发育为仔虾。     

工厂化育苗中根域容积对万寿菊幼苗生长的影响

以万寿菊色素专用品种'99146'(TageteserectaL.cvScarletade)为试验材料,采用50孔、72孔和128孔穴盘,从幼苗的生长量、根系活力及生理苗龄期等方面入手,探讨根域容积对幼苗生长的影响。结果表明:50孔、72孔和128孔穴盘育成的幼苗均生长良好,根域容积对万寿菊幼苗营养生长无显著影响。在实际生产中,根据成本及技术,建议采用72孔穴盘。     

速收除草肥在胡麻田的药效及安全性试验

采用随机区组设计方法,就速收除草剂与肥料混用在胡麻田中的药效及安全性作了盆栽试验,对不同速收除草肥浓度下胡麻及其模拟杂草的出苗、株高、鲜重等形态指标及胡麻的叶绿素、硝酸还原酶、POD、可溶性糖等生理指标进行了测定分析,结果表明：速收(180g/hm2)与肥料混用对胡麻田有较好的安全性不会对胡麻产生危害,可作为胡麻田良好的除草肥。     

渭北果区果-菇高效生态农业模式研究

概述了渭北果区利用果树枝杆生产香菇,既取得了显著的经济效益,又变废为宝,综合利用,取得了显著的生态效益,形成“果—菇—饲—沼—肥”的高效生态农业模式。     

杂种小麦籽粒成熟期间的优势性状研究

本文通过对杂种小麦(CHA 杂种)和普通小麦品种的比较研究,分析了杂种小麦在籽粒成熟过程中的几个优势性状。结果表明:杂种小麦顶部叶片的光合速率,光合速率高值持续期和有效叶面积等光合特性均优于普通小麦品种。在光合速率日变化方面,杂种小麦和普通小麦品种的最高值差异显著,而最低值则差异不大,表现出杂种小麦对强光的适应力强,具较大优势;而在弱光下,则难以发挥优势的特性。在籽粒增重上,杂种小麦也表现出灌浆速率大,高值持续时间长等特点。矮秆大穗型杂种小麦的产量因素构成则表现为每穗粒数多或粒重高,穗粒重高的显著优势。