首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   250547篇
  免费   111356篇
  国内免费   7290篇
林业   23786篇
农学   16915篇
基础科学   20295篇
  53498篇
综合类   138077篇
农作物   20417篇
水产渔业   14901篇
畜牧兽医   44685篇
园艺   20217篇
植物保护   16402篇
  2025年   3441篇
  2024年   7520篇
  2023年   7651篇
  2022年   12106篇
  2021年   11594篇
  2020年   12129篇
  2019年   14116篇
  2018年   11181篇
  2017年   15745篇
  2016年   13153篇
  2015年   17212篇
  2014年   16755篇
  2013年   19064篇
  2012年   24416篇
  2011年   24298篇
  2010年   22667篇
  2009年   20451篇
  2008年   20094篇
  2007年   18555篇
  2006年   15233篇
  2005年   12458篇
  2004年   8665篇
  2003年   6006篇
  2002年   5922篇
  2001年   5197篇
  2000年   4820篇
  1999年   2550篇
  1998年   1478篇
  1997年   1410篇
  1996年   1202篇
  1995年   1281篇
  1994年   1258篇
  1993年   1014篇
  1992年   1041篇
  1991年   869篇
  1990年   661篇
  1989年   645篇
  1988年   582篇
  1987年   454篇
  1986年   459篇
  1985年   229篇
  1984年   213篇
  1983年   198篇
  1982年   196篇
  1981年   266篇
  1980年   195篇
  1979年   158篇
  1978年   134篇
  1965年   148篇
  1964年   154篇
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
71.
An ontogenetic study was designed on developing rats in uterus of mothers tryptophan deprived at day 1 (exp. 1) and day 14.5 (exp. 2) of conception to verify the supposed determining role of the serotoninergic system (SS) in sexual differentiation in mammals. Tryptophan-free feeding was pursued uninterruptedly in the litter after birth, during lactation and post-natal development. Tryptophan-free pregnant rats were obtained by exclusion of tryptophan sources from chow. In both exp. 1 and exp. 2, the litter showed at birth a significant physical under evolution that worsened, during post-natal development, to a much more marked dwarfism in exp. 1 pups. Growth hormone concentrations in both sexes of dwarf rats were lower than that in the control rats. At 30 days post-natal age, whereas exp. 1 female rats showed a right-timed onset of puberty, no descensus of testes could be observed in male rats of same experiment. Dwarf male rats showed an evident hypotrophy of the whole reproductive apparatus. In histological examination of testes, neither spermatogenesis nor Leydig cells have been observed. Moreover, dwarf female rats showed a pronounced hypotrophy of reproductive organs, but a normal puberal status pattern was evident. In exp. 2, litters showed a less pronounced dwarfism, but a normal right-timed onset of puberty in both male and female rats. Data indicate that role of tryptophan in physical and sexual maturation in both male and female rats is essential.  相似文献   
72.
Maedi visna virus (MVV) and caprine arthritis encephalitis virus (CAEV) are a heterogeneous group of infectious agents affecting sheep and goats. Due to their natural cross-species infection they are referred to as small-ruminant lentiviruses (SRLV). Recently a new genetic cluster, highly divergent from MVV and CAEV was identified in the north-west part of Italy. A panel of genotype E specific antigens was developed and evaluated in flocks infected with B1 and E strains. The results clearly indicate that a strain specific antigen is required to correctly identify animals infected with different genotypes.  相似文献   
73.
News item     
  相似文献   
74.
75.
对玉溪市65家野生动物驯养繁殖单位的基本情况和疫源疫病监测防疫情况进行调查,收集数据进行汇总分析,从驯养繁殖种类及规模、技术人员配备、卫生防疫及消毒设施配备及消毒对象、人员防护、检疫、免疫、无害化处理、屠宰、信息档案等方面探讨玉溪市在野生动物驯养繁殖场所疫源疫病监测防疫中存在的风险和隐患.认为目前尚存在着对疫病传播的危害性认识不足,设施设备配置不全面,技术能力欠缺,尚未形成长期有效的监测防疫体系等问题,藉此提出加强宣传,增强意识;增加必要的投入;搭建技术平台,提供专业指导;完善监测防疫体系,形成长效机制等建议.  相似文献   
76.
  总被引:1,自引:1,他引:1  
A biotin/avidin double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of antigens of Leptospira interrogans serovars in experimentally inoculated bovine urine samples was evaluated. Immunoglobulin G (IgG) from rabbits immunized with L. interrogans serovar hardjo type hardjobovis sonicated, whole cell, and formalinized-heated antigen preparations were purified by a protein A-superose column coupled to fast protein liquid chromatography, and evaluated for species specificity in the ELISA. The ELISA using each specific IgG detected as few as 10(4) leptospires of the homologous serovar hardjo diluted in phosphate-buffered saline solution with Tween 20 (PBSS-Tween 20). On immunoblot analysis of proteinase-K-digested whole cell leptospiral preparations, each IgG revealed the presence of bands specific to serovar hardjo, suggesting the presence of serovar-specific epitopes on the lipopolysaccharide molecules. The minimum number of cells of heterologous serovars pomona, grippotyphosa, bratislava, icterohaemorrhagiae and copenhageni detected by each ELISA was greater, ranging from 10(6) to 10(7). The common antigenic determinants observed on immunoblot analysis were different for each specific IgG, except for a major cross-reacting, possibly flagellar, protein doublet at approximately 36-36.5 kDa. Leptospires were equally well detected by the ELISA in both bovine urine and PBSS-Tween 20.  相似文献   
77.
从翻地、整地、农药、种子、肥料等方面对旱作水稻与常规水稻、旱作水稻与玉米进行经济效益比较。总结出旱作水稻所具有的省工省肥、耐盐碱、低投入高产出等特点,阐述了发展旱稻生产的意义。  相似文献   
78.
Objective: To describe diagnostics, therapy, and sequelae of acute carbon monoxide (CO) toxicity because of a motor vehicle generator in 4 dogs and 2 cats. Series summary: Four dogs and 2 cats presented for recumbency, disorientation, dyspnea, and stiffness after an estimated 6–8 hour exposure to exhaust from a generator. Diagnostics included a serum carboxyhemoglobin levels evaluation, arterial blood gas analysis, pulse oximetry readings, and blood pressure measurements. Initial therapy included oxygen (O2) administration, intravenous bronchodilators, fluids, and a hemoglobin‐based O2 carrying (HBOC) molecule. Following administration of the HBOC, 4 of the 6 animals showed dramatic clinical improvement. Two weeks after hospital discharge, the owner reported potential hearing deficits in all animals. Brain auditory evoked response (BAER) tests were conducted in all surviving animals and some degree of hearing impairment was documented in all cases, with complete clinical resolution noted 6 weeks later. Unique information provided: This report describes the therapeutic use of an HBOC in acute isolated CO toxicity (i.e. without the complications of smoke inhalation). In addition, delayed nervous system dysfunction was documented in all surviving animals.  相似文献   
79.
  总被引:6,自引:0,他引:6  
Fecal samples from diarrheic and nondiarrheic dairy calves (1 to 3 weeks old) from 12 regions of Quebec, collected between 1992 and 1994, were screened for group A bovine rotavirus (BRV) using a combination of 2 VP6-specific monoclonal antibodies (MAbs) in an enzyme-linked immunosorbent assay (ELISA). The overall prevalence of BRV infection was 26.4% (107/405). In diarrheic calves, BRV infection reached 74.3% (55/74), but only 15.7% (52/331) in nondiarrheic calves. BRV-positive samples were serotyped by enzyme-linked immunosorbent assay (ELISA) using G6 and G10 specific MAbs. The analysis of 107 field samples revealed that, in diarrheic calves, 34.5% (19/55) were G6, 27.2% (15/55) were G10, 9% (5/55) were G6 and G10 positive, and 29.9% (16/55) were G6 and G10 negative. In nondiarrheic calves, 19.2% (10/52) were G6, 19.2% (10/52) were G10, 7.6% (4/52) were G6 and G10 positive, and 53.6% (28/52) were G6 and G10 negative. Rotavirus dsRNA was extracted from BRV-positive samples and examined by polyacrilamide gel electrophoresis (PAGE). Of 107 samples tested, 74 (69.1%) were positive, and all the samples demonstrated a typical group A rotavirus migration pattern.  相似文献   
80.
  总被引:1,自引:0,他引:1  
  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号