pH对产叶酸植物乳杆菌叶酸产量及相关基因表达的影响

叶酸是细胞代谢的重要中间体,人类由于缺乏叶酸生物合成途径中的关键酶,需要从膳食中摄取叶酸。由于谷氨酸尾数目的差别,生物合成的叶酸更容易被人体利用。一些植物乳杆菌菌株可以合成叶酸,但培养条件对叶酸合成有较大影响。选择一株高产叶酸的植物乳杆菌4_3菌株,探讨了在同一培养基中,不调节pH和使pH稳定在6.0对其生长状况和叶酸合成的影响。比起不调节pH,维持pH 6.0可以长期保持较高的活细胞密度。实时荧光定量PCR的结果显示,在pH 6.0的培养基中,叶酸合成相关基因表达量均要高于不调节pH的培养基。     

自行式牛蒡播种机关键部件的有限元仿真与设计

牛蒡作为一种重要的经济作物而被广泛的种植生产,但在其播种作业中,主要依靠人力,生产效率低,播种效果不理想,人力成本高。为了解决上述问题,设计了一种自行式牛蒡播种机,以实现播种的半自动化。通过UG建立整体模型,导入虚拟仿真平台,验证机构的合理性及可行性。为此,基于有限元分析软件ANSYS workbench,对重要受力部件部件进行静力学与模态仿真,验证关键部件的强度刚度是否满足实际工作需要。静力学分析结果表明:该机可以满足牛蒡播种的工作要求。最后,通过参数优化选择试制试验样机,进行实际检验,得出结论:播种滚子满足实际生产中的强度与刚度需要。     

The response of microbial composition and enzyme activities to hydrological gradients in a riparian wetland

Journal of Soils and Sediments - Hydrological condition is one of the important factors impacting microbial structure in riparian wetlands. However, how these microbial communities respond to...     

内蒙古图牧吉国家级自然保护区雁形目鸟类时空分布特征

文章以了解分析内蒙古图牧吉国家级自然保护区雁形目鸟类种类及分布特征并更好地保护管理的目的,于2017年4月至2018年1月份春、夏、秋、冬四个季节,采用样线和样点统计法,对保护区图牧吉泡子和三道泡子两处湖泊湿地雁形目鸟类种类和数量进行调查统计。结果显示:共观察记录到雁形目鸟类22种,隶属于1科8属。图牧吉泡子和三道泡子分别观察到19种和14种。两处湖泊湿地之间距离虽然较近,但雁形目鸟类种类有较大的区别,其中图牧吉泡子不仅种类多,而且珍稀濒危物种的分布也较集中。保护区雁形目鸟类时空分布特征与两处湖泊湿地各自食物资源、隐蔽条件和人为干扰程度有关外也与鸟类本身的迁徙规律和种间竞争有着密切关系。     

基于Workbench的某转子发动机偏心轴强度分析

以某转子发动机的偏心轴为研究对象,对偏心轴的振动及最大爆发压力下的瞬态冲击应力进行了分析。运用CATIA软件建立偏心轴三维模型,将模型输入ANSYS Workbench进行模态和强度分析,得出了输出偏心轴的前6阶固有频率和及偏心轴在最高爆发压力下的瞬态冲击应力。结果表明,偏心轴的最低阶固有频率高于偏心轴的激励频率,最大爆发压力下的极限应力发生在轴承段与主轴颈的过渡圆角处。     

青冈栎林分空间结构对林下更新的影响

林分空间结构会显著影响其林下幼树更新。为明确其影响林下幼树更新的关键因子,以湖南省青羊湖国有林场青冈栎林天然次生林为研究对象,测量幼树株数密度、树高、地径和冠幅,计算混交度、大小比数、开敞度指数以及聚集指数代表林分空间结构,评价林下幼树更新情况,并用灰色关联度分析方法探究两者间的相关关系。结果表明,青羊湖国有林场13块青冈栎林样地林下更新幼树有6种,分属于4科5属,分别是青冈栎、苦槠、多脉青冈、南酸枣、黄檀、马尾松,青冈栎在更新幼树中占明显优势,是优势树种,幼树重要值为63.09%。青冈栎林各林分空间结构指数与林下更新指数均大于0.5,关联度较高,综合所有林分空间结构指数对林下更新指数关联度的均值来看,对林下更新的影响由大到小为大小比数>开敞度>聚集指数>混交度,大小比数对青冈栎林下更新影响最为显著。在促进青冈栎天然次生林林下更新时,应以调整林木大小分化程度为主,综合考虑林木空间分布格局的调整方案。     

基于超星学习通的混合式教学模式应用研究——以发酵工程课程为例

为了提高发酵工程课程的教学质量和教学效果,针对发酵工程课程的内容和特点,将互联网+技术与教学相结合,进行了基于超星学习通平台的混合式线上线下教学方式的改革,结果表明:在增强了学生自主学习能力的同时,也促进了教师的自我提升。     

Effect of Sodium Chloride on Meltability of Mozzarella Cheese

Meltability is one of the most important properties of Mozzarella cheese, as it is generally used in pizza and other foods. Mozzarella was prepared by no salted and immature production technology, and the effect of different addition amounts of salt on the meltability of mozzarella cheese was measured by Schreiber method and small amplitude oscillatory shear analysis（SAOSA） method. The results showed that different adding amounts of NaCl had significant influence on the meltability of Mozzarella cheese, and 2% NaCl addition was the best condition. The results measured by the methods of Schreiber and SAOSA were basically same： adding different amounts of NaCl had significant influence on the hardness and elasticity of mozzarella cheese, but no significant influence on the sticky. It was a good microscopic structure arrangement of Mozzarella cheese with 2% NaCl addition. Scanning electron micrographs showed that a space grid structure formed by casein had changed, and formed many uniform molecular holes. The results indicated that different addition amounts of salt had influence on meltability of no salted immature Mozzarella cheese, and this technology could be drastically shorten the processing time.     

Variation at the insulin-like growth factor 1 gene and its association with body weight traits in the chicken

The insulin-like growth factor 1 (IGF1) is essential for normal embryonic and postnatal growth in mammals. In this study, a total of 342 F(2) individuals, derived from Broiler crossing to Baier layer (Northeast Agricultural University Resource Population, NEAURP), were used to investigate the associations of haplotypes in the chicken IGF1 (cIGF1) gene with body weight traits. Primers for the 5'-flanking, exon 3 and 3'-flanking regions of cIGF1 were designed according to chicken genome database. Single nucleotide polymorphisms (SNPs) between parental lines were detected by sequencing, and PCR restriction fragment length polymorphism (PCR-RFLP) and PCR single-stranded-conformation polymorphism (PCR-SSCP) methods were used to genotype the SNPs in the population. Haplotypes were constructed with the three SNPs detected. The association analysis showed that haplotypes based on three cIGF1 polymorphisms (c.-366A>C, c.528G>A and c.*1024C>T) were associated with body weight traits, suggesting that cIGF1 or a tightly linked gene had effects on body weight in the chicken.