Research on Lead-time Compression of Inventory Integrating Remanufacturing and Manufacturing System for the IOT Environment

The lead time compression is the core of supply chain management with time competition and the powerful source of competitive advantage of supply chain.As an emerging technology,The Internet of things ...     

FGF4在肺癌细胞体内和体外中表达差异的研究

FGF4已经被证明是癌基因,它涉及肿瘤的生长和转移,为了解FGF4的表达与肿瘤微环境的关系。我们利用FGF4抗体通过免疫组化对一名肺癌患者癌旁,癌组织,癌组织小鼠移植瘤,二次移植瘤以及原代培养的细胞爬片进行FGF4检测,探究其表达差异。通过对比癌组织与对照组（癌旁组织）,FGF4在癌巢中高表达;同样,移植瘤与二次移植瘤的癌巢中与癌旁组织比较,FGF4表达相对较高;但是将癌组织进行原代培养后,免疫组化检测细胞爬片FGF4,发现仅有5%±0.21%的肿瘤细胞表达FGF4,对照蛋白Cytokine作为肿瘤标记物,则在100%的肿瘤细胞中表达。研究提示免疫组化检测到FGF4在体内和体外表达不同,提示肿瘤细胞FGF4的表达与肿瘤微环境调控密切相关,肿瘤微环境对肿瘤细胞的FGF4的调控有着重要作用。     

湖南省R&D投入产出效率研究

以湖南省R&D投入产出活动为研究对象，采用数据包络分析法(DEA)的BCC模型，结合全国30个省市R&D投入产出数据，对2013年湖南省R&D投入产出效率进行比较分析，并根据实证结果提出相关对策及建议.     

新疆多浪羊FecB突变检测及与产羔数的关系

骨形态发生蛋白受体IB基因(BMPR-IB)FecB突变是影响布鲁拉美利奴和小尾寒羊高繁殖力的主效基因.本研究采集产双羔及以上的多浪羊(Ovis aries)母羊135只及后代羔羊血样共353份,公羊血样211份,利用PCR-RFLP技术检测FecB突变,并分析不同基因型个体母羊的产羔数.结果表明:在多浪羊群体中检测到BB、B+和++基因型,基因型频率分别为0.005,0.146和0.849.B+型多浪羊产羔数均值比++型多0.51只(P<0.01),BB型多浪羊产羔数分别与B+和++型差异不显著.多浪羊群体在FecB位点上处于Hard-Weinberg平衡状态,表明在该位点上没有受到选择的影响.本研究首次在新疆多浪羊群体中发现了FecB突变BB基因型公、母羊个体(1只公羊和2只母羊),揭示多浪羊多羔产生的机理可能与布鲁拉美利奴和小尾寒羊相同,在生产过程中可以根据携带FecB突变的情况开展选种选配,组建多浪羊肉用高繁品系.     

Regulatory effect of salicylic acid and methyl jasmonate supplementation on ergosterol production in Hericium erinaceus mycelia

We evaluated the biomass and ergosterol content of Hericium erinaceus mycelium, and extracellular enzyme activities in H. erinaceus liquid culture following salicylic acid(SA) and methyl jasmonic acid(Me JA)supplementation. The optimal SA concentration was100 lmoláL-1, where the highest ergosterol content of 2.33 mgág-1was obtained following 6-day cultivation with100 lmoláL-1SA supplementation, and which was significantly higher than the unsupplemented control(p \ 0.01). Following 4-day supplementation with50 lmoláL-1Me JA, the highest ergosterol content obtained was 1.988 mgág-1, which was 25.8 % higher than the unsupplemented control. Our data indicate that SA and Me JA supplementation improves ergosterol content in H.erinaceus mycelium.     

金中都公园的园林景观与历史文化展示

本文试以北京金中都公园设计和建设过程为例,论述如何将园林景观与历史文化的合理展示相结合。提出设计师的创作和实践应该与建设过程相配合,以匠人精神应对施工阶段的现场完形和设计提升。总结了设计项目从概念规划直至建成,设计师的全过程设计的重要性和必要性。探讨了景观与艺术专业的协同设计在文化表达和景观展示中的叠加效应。提出设计师对场地的挖掘和设计推敲,应该随着项目的施工建设进度并以现场设计为核心,并举例论述了现场调整对提升设计品质的重要作用。以期为公园改造和园林景观设计师的工作实践提供有益的参考和引导。     

漳州市加快发展对台农业交流合作政策选择的研究

随着ECFA的实施,漳台(漳州市、台湾省,下同)农业资源将进一步得到合理配置,漳台农业交流合作的空间和领域将得到更大的拓展。漳州市如何在现有的基础和成效上抓住这些有利时机,突破制约漳台农业进一步合作的瓶颈,努力促进漳台农业合作实现跨越式发展,具有重大的现实意义和深远的历史意义。     

温室黄瓜新品种中农33号的选育

中农33号是以自交系09676和12173为父母本配制的黄瓜一代杂种。植株生长势强,耐低温弱光性好。果实商品性好,颜色深绿、有光泽,腰瓜长约35 cm,瓜粗3.4 cm左右,瓜把长约4.5 cm,心腔较小,果肉为淡绿色。白刺、密,瘤小,无棱。高抗WMV,中抗CGMMV、枯萎病、黑星病。早熟性好,持续结果及丰产优势明显。温室长季节栽培每667 m~2产量高达10 000 kg以上。适宜日光温室和春棚栽培。     

Mycoplasma pneumoniae induces IL-1β production through activating NLRP3 inflammasome by ROS in RAW264.7 cells

AIM: To investigate whether Mycoplasma pneumoniae (Mp)-induced interleukin-1β (IL-1β) production in RAW264.7 cells is through the activation of NLRP3 inflammasome via reactive oxygen species (ROS). ME-THODS: RAW264.7 cells were randomly divided into 3 groups. In normal group, RAW264.7 cells were treated without Mp. In model group, RAW264.7 cells were treated with 1∶ 10 multiplicity of infection (MOI) of Mp. In NAC group, RAW264.7 cells were pretreated with N- acetylcysteine (NAC) at a concentration of 5 mmol/L for 30 min before infection with Mp. The RAW264.7cells were infected with Mp (1∶ 10 MOI) for 4, 8, 16 and 24 h in model group and NAC group, respectively. The intracellular ROS level was analyzed by flow cytometry. The mRNA expressions of NLRP3, ASC and caspase-1 were detected by real-time PCR. The protein levels of NLRP3, ASC and caspase-1 p20 were determined by Western blot. The levels of pro-inflammatory cytokine IL-1β in the supernatant were measured by ELISA. RESULTS: Compared with normal group, the production of ROS were significantly increased at 4, 8, 16 and 24 h after infection, the mRNA expression of NLRP3, ASC and caspase-1 were increased at 8, 16 and 24 h after infection, the protein levels of NLRP3, ASC and caspase-1 p20 were increased at 16 and 24 h after infection, and the releases of IL-1β were increased at 24 h after infection in model group (P<0.01). Compared with the model group, the level of ROS in NAC group decreased, so as the expression of NLRP3, ASC and caspase-1 at mRNA and protein levels and the releases of IL-1β in the supernatant at the corresponding time points. CONCLUSION: Mp may stimulate the ROS production to activate NLRP3 inflammasome in RAW264.7 cells.     

农机车载电子设备用户界面框架设计

介绍了一种农机车载电子设备显示界面框架的设计与实现。基于C语言分析设计了界面框架的数据结构,使用分层的数据结构设计方法实现了多类型数据的显示需要和灵活的界面类型组合方式,以极少的开销实现了多级菜单。将内容数据与操作函数分离,解决了操作函数复用的问题,同时提高了程序的可维护性。提出的新型的界面显示框架实现了多种农业装备常见的界面应用类型,解决了小屏幕嵌入式系统显示界面移植性差问题。