TonB is essential for virulence in avian pathogenic Escherichia coli

Avian pathogenic Escherichia coli (APEC) strains have multiple iron-uptake systems that facilitate adaptation to iron-restricted environments and are believed to assist in colonisation of the host. These systems include several TonB-dependent transporters of ferri-siderophores encoded by the chromosome and the large virulence plasmid common to APECs. The tonB gene of the virulent APEC strain E956 was replaced with a selectable antibiotic resistance marker using Lambda Red recombinase mutagenesis. The phenotype of the ΔtonB E956 mutant was compared to the parent strain under various culture conditions and in chickens experimentally infected via the respiratory route. The mutant was resistant to streptonigrin, impaired in its ability to adapt to growth in iron-depleted medium and had greater tolerance of oxidative stress than the parental strain. The mutant was avirulent in chickens, did not affect the growth of chicks and colonisation was mostly limited to the trachea. This study has demonstrated that TonB is essential for virulence in APEC.     

Duration of immunity with Mycoplasma synoviae: comparison of the live attenuated vaccine MS-H (Vaxsafe MS) with its wild-type parent strain, 86079/7NS

The duration of protective immunity elicited by the MS-H vaccine was evaluated by experimental challenge of chickens at 15 and 40 wk after eyedrop vaccination. Immunity induced by the parent strain of the vaccine, 86079/7NS, was also investigated for comparison. A serological response to Mycoplasma synoviae was detected in 89% to 100% of MS-H vaccinates and 86079/7NS inoculates at 15, 27, 30, 35, and 40 wk after inoculation. A significantly lower incidence of air-sac lesions and lower air-sac lesion severity were observed in both the MS-H vaccinated and the 86079/7NS inoculated groups, as compared to the unvaccinated controls, after both challenge points. Tracheal mucosal thicknesses in MS-H vaccinates was significantly lower in the upper, lower, and total trachea at 40 wk after vaccination, as compared to the controls. It was demonstrated in this experiment that protective immunity, as determined by protection against experimental challenge, was maintained to at least 40 wk after vaccination.     

Effect of combined heat and high-pressure treatments on the texture of chicken breast muscle (pectoralis fundus)

Commercially supplied chicken breast muscle was subjected to simultaneous heat and pressure treatments. Treatment conditions ranged from ambient temperature to 70 degrees C and from 0.1 to 800 MPa, respectively, in various combinations. Texture profile analysis (TPA) of the treated samples was performed to determine changes in muscle hardness. At treatment temperatures up to and including 50 degrees C, heat and pressure acted synergistically to increase muscle hardness. However, at 60 and 70 degrees C, hardness decreased following treatments in excess of 200 MPa. TPA was performed on extracted myofibrillar protein gels that after treatment under similar conditions revealed similar effects of heat and pressure. Differential scanning calorimetry analysis of whole muscle samples revealed that at ambient pressure the unfolding of myosin was completed at 60 degrees C, unlike actin, which completely denatured only above 70 degrees C. With simultaneous pressure treatment at >200 MPa, myosin and actin unfolded at 20 degrees C. Unfolding of myosin and actin could be induced in extracted myofibrillar protein with simultaneous treatment at 200 MPa and 40 degrees C. Electrophoretic analysis indicated high pressure/temperature regimens induced disulfide bonding between myosin chains.     

Toxicity of selected biorational insecticides to Pulvinaria aurantii Cockerell and its predator,Cryptolaemus montrouzieri Mulsant in citrus field

The citrus cottony scale, Pulvinaria aurantii Cockerell, is the most important pest of citrus orchards in northern Iran in recent years. Owing to the harmful impacts of chemical insecticides, the use of biorational pesticides seems a safe and convenient way to control this pest. The present investigation was set up to study contact toxicity of various biorational and chemical compounds on the first, second instar nymph and overwintering adult of the citrus cottony scale, P. aurantii, and its predator, Cryptolaemus montrouzieri Mulsant under field conditions. The results indicated that the highest percentage of mortality of first instar nymph of the citrus cottony scale was observed in the Dayabon treatment of 9 and 10?ml/L, 100.0%?±?0.0% and the lowest percentage was observed in Palizin treatment of 1.5?ml/L, 34.7%?±?2.9%. In the second instar nymph, the highest mortality was seen in the Dursban treatment of 2?ml/L, 94.7%?±?2.4% and the lowest mortality in the Palizin treatment of 1.5?ml/L, 30.0%?±?2.3%. Also, for overwintering adults Dayabon 10?ml/L caused the highest mortality, 96.0%?±?4.0% during 24?h. In the case of ladybird insects, the highest and lowest percentage of mortality was observed in the Dursban treatment of 2?ml/L, 76.7%?±?8.8%, Dayabon 5?ml/L, and Palizin 2?ml/L, 3.3%?±?3.3%, respectively. The highest and lowest mortality rate of larvae of ladybird was estimated in the Dursban treatment of 2?ml/L, 83.3%?±?3.3% and Dayabon 5?ml/L, 3.3%?±?3.3%, respectively. Also, for overwintering predator adults Palizin 1.5?ml/L had the lowest mortality, 3.3%?±?3.3% during 24?h and this rate remain unchanged after 96?h after treatment. According to the results obtained, treatments with Dayabon 9 and 10?ml/L, can be a viable alternative to other high-risk chemical pesticides in addition to effectively controlling citrus cottony scale without having any adverse impacts on predator ladybird.     

Removal of Chromium by Coagulation-Dissolved Air Flotation System Using Ferric Chloride and Poly Aluminum Chloride (PAC) as Coagulants

In this study, dissolved air flotation (DAF) was examined as a possible treatment method for the removal of chromium from aqueous solution and plating wastewater. Two coagulants, ferric chloride and poly aluminum chloride (PAC), were used for pretreatment of wastewater. Maximum removal of chromium was achieved for poly aluminum chloride (98 %). Artificial neural network was used for the prediction of the DAF system. The best neuron used for the prediction of chromium removal percentage of interpolated wastewater was 6 %. The mean score error and the coefficient correlation were 0.0007542 and 0.997, respectively.     

Effect of microbial decomposition of mature leaves on soil pH

The hypothesis that the microbial decomposition of organic matter low in organic nitrogen leads to an increase in soil pH was tested and confirmed in a pot experiment with two different soils into which mature cherry leaves were incorporated.     

Aggressiveness of eight <Emphasis Type="Italic">Didymella rabiei</Emphasis> isolates from domesticated and wild chickpea native to Turkey and Israel,a case study

Ascochyta blight, caused by Didymella rabiei, affects both domesticated chickpea and its congeneric wild relatives. The aim of this study was to compare the aggressiveness of D. rabiei isolates from wild and domesticated Cicer spp. in Turkey and Israel on wild and domesticated hosts from both countries. A total of eight isolates of D. rabiei sampled from C. pinnatifidum, C. judaicum and C. arietinum in Turkey and Israel was tested on two domesticated chickpea cultivars and two wild Cicer accessions from Turkey and Israel. Using cross-inoculation experiments, we compared pathogen aggressiveness across the different pathogen and host origin combinations. Two measures of aggressiveness were used, incubation period and relative area under the disease progress curve. The eight tested isolates infected all of the host plants, but were more aggressive on their original hosts with one exception; Turkish domesticated isolates were less aggressive on their domesticated host in comparison to the aggressiveness of Israeli domesticated isolates on Turkish domesticated chickpea. C. judaicum plants were highly resistant against all of the isolates from different origins except for their own isolates. Regardless of the country of origin, the wild isolates were highly aggressive on domesticated chickpea while the domesticated isolates were less aggressive on the wild hosts compared with the wild isolates. These results suggest that the aggressiveness pattern of D. rabiei on different hosts could have been shaped by adaptation to the distinct ecological niches of wild vs. domesticated chickpea.