Arbuscular mycorrhizal fungi improve the growth and phosphorus uptake of mung bean plants fertilized with composted rock phosphate fed dung in alkaline soil environment

Abstract

Inoculation effect of arbuscular mycorrhizal fungi (AMF) on phosphorus (P) transfer from composted dung of cattle with a diet supplemented with powdered rock phosphate (RP) and their successive uptake by mung bean plants was assessed in alkaline soil. The efficacy of composted RP fed dung alone or/and in combination with AMF inoculums containing six different species were compared with SSP in six replicates per treatment in pots. The results showed that the association of AMF with composted RP fed dung had a positive effect on mung bean shoot (3.04?g) and root (2.62?g) biomass, chlorophyll (a, b), carotenoid contents and N (58.38?mg plant^?1) and P (4.61?mg plant^?1) uptake. Similarly, the percent roots colonization (56%) and nodulation of mung bean plant roots and their post-harvest soil properties were also improved by the inoculation of AMF together with composted RP fed dung. It is concluded that the combined application of AMF with composted RP fed dung has almost the same effect as SSP for improving mung bean plants growth and their nutrients uptake. Moreover, AMF inoculants can be used as a suitable biofertilizer in combination with locally available organic sources of fertilizers for improving P status and growth of plants in alkaline soils.     

Mycorrhizal inoculation enhances growth and nutrition of cotton plant

Arbuscular Mycorrhizae Fungi (AMF) inoculations may improve growth and nutrient uptake of cotton (Gossypium hirsutum L.) plant. Although the importance of mycorrhizal symbioses for growth and nutrient acquisition of cotton plant is known, less is known about mycorrhizal dependency on P and Zn nutrition under low Zn fertile soil conditions. A greenhouse experiment was conducted to investigate the effect of different of P and Zn fertilizer addition on cotton plant growth as well as Zn and P uptake. Sterilized and non-sterilized low Zn fertile Konya series soil was treated with different levels of P and Zn. Soils were inoculated with two mycorrhizae species like Funneliformis mosseae and Claroideoglomus etunicatum after sterilization. Results showed that mycorrhizal inoculation on plant growth and nutrient uptake has significant effect when soil was sterilized. Cl. etunicatum mycorrhizae species has greater effect than Fu. mosseae mycorrhizae species. Root colonization increased 23–65% due to mycorrhizal amendment. The shoot: root ratio increased by 13 and 22% for non-sterile and sterile condition respectively in mycorrhiza amended soil. Mycorrhizal dependency varies 1–55% and 3–64% for non-sterile and sterile soil respectively on mycorrhizae, P and Zn amended soil. Mycorrhizal dependency analysis showed that cotton plant in both sterile and non-sterile soil conditions depends on mycorrhizae species, P nutrition, however is less depend on Zn nutrition. This study concluded that the inoculation of cotton plant with selected mycorrhizae is necessary under both sterile and non-sterile soil conditions.     

Alleviation of alternate bearing phenomenon in mango (Mangifera indica L.) trees using boron and nitrogen fertilization

Abstract

This investigation was conducted during two successive seasons (2014/2015) and (2015/2016) using 15?years old productive mango (Mangifera indica) trees cv. Zebda. The trees were grown at AlMalak Valley Farm, El-Sharkeya Governorate- Egypt (30–51° North; 32–53° East). Trees were planted 8?×?8 meter within and between rows in sandy soil under drip irrigation system using the Nile water. The objective of this study is to alleviate alternate bearing in cv. Zebda using mineral nutrients (nitrogen in the on year and boron in the off year). Treatments included three concentrations of nitrogen (1000, 1250, 1500?g/tree/year) and three concentrations of boron (0.0, 250, 500?mg L^?1). Nitrogen was applied to the soil as ammonium sulfate and boron was applied as foliar spray of boric acid. The extra amount of nitrogen fertilizer (250 and 500?g N/tree) was applied at three installments in (May, June and July). Treatment was arranged in a factorial Completely Randomized Block Design with three replicates for each treatment. Results show that the on-year nitrogen fertilization significantly increased mango tree vegetative growth (number of shoot/branch, shoot length, shoot thickness, number of leaves/meter and leaf area) and yield. The average yield in the on year is 85.5?kg/tree at 1250?g N/tree but 67.4?Kilogram/tree at 1000?g N/tree (the control treatment). While in the off year boron foliar application resulted in a significant increase in flowering, initial fruit set, final fruit set and fruit yield. The average yield in the off year is 47?kg/tree at 250?mg L^?1 boron but 9?kg/tree at 0.0?mg L^?1 boron rate (the control treatment).The interaction treatment of 250?mg L^?1 boron + 1500?g nitrogen/tree is the best treatment as it resulted in the highest values for all the tested parameters. The average yield of this treatment is 53.5?kg/tree. This treatment helps alleviate alternate bearing phenomenon by 41% and obtain the highest economic yield in the off-year, i.e. increased yield by 5.9 fold.     

Critical nutrient concentrations and antagonistic and synergistic relationships among the nutrients of NFT‐grown young tomato plants

Critical concentrations of nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), iron (Fe), zinc (Zn), and manganese (Mn) with respect to dry matter yield end antagonistic and synergistic relationships among these nutrients were studied in which tomato (Lycopersicon esculentum L.) was grown in recirculating nutrient solution (NFT). Increments of nutrient elements in the nutrient solution increased the proportional rate of the corresponding nutrient elements. Increasing levels of N negatively correlated with plant P and positively correlated with Ca, Fe, and Zn. Iron and Mn contents of the plants were increased and N, K, Ca, and Mg were decreased as a function of P applied. Increases in K in the nutrient solution caused increases in the concentrations of K, N, P, and Zn, and decreases in the concentration of Ca and Fe. Applied Ca increased the concentrations of Ca and N, and decreased the concentrations of P, Mg, Fe, Zn, and Mn. Potassium, Ca, and Fe contents of the plants were decreased and Zn increased, while N, P, and Mn were not affected by the increasing levels of external Mg. Iron suppressed the plant Mg, Zn, and Mn contents. Synergism between Zn and Fe was seen, while P, K, Ca, Mg, and Mn contents were not affected by Zn levels. Potassium, Ca, Mg, and Fe were not responsive to applied Mn, however, N and P contents of the plants were decreased at the highest levels of Mn.     

Simulating the Production of Rice Genotypes by Flood Management and End-Season Water Stress Conditions Using AquaCrop Model

ABSTRACT

The present study was conducted to assess the ability of AquaCrop model in predicting of grain and biological yield of rice genotypes in water management. A two-year field experiment was conducted at the experimental farm of the Iranian Rice Research Institute in Rasht, Iran from 2016 to 2017. The experiment was established in a split-plot design with two irrigation management (continuous submergence and end season water stress) as the main plot, fourth rice genotypes as the sub-plot and three replications. The goodness-of-fit between observed and simulated grain yield and final biomass was assessed by means of the coefficient of determination (R ²), the absolute and normalized root mean square errors (RMSE). The RMSE_n of predicting grain yield at calibration and evaluation stages was in the range of 6–12% and 6–8% for biological yield. The results indicated that AquaCrop model is suitable to predict grain yield and biological yield of rice genotypes in northern Iran. AquaCrop model can be used to determine optimization strategies to improve the water consumption of rice genotypes.     

Nutrients management strategies to improve yield and quality of sugar beet in semi-arid regions

The objective of this study was to evaluate the effects of organic and inorganic fertilizers on the yield and quality of sugar beet genotypes (Beta vulgaris L.). Therefore, a field trial was carried out in Peshawar, Pakistan, during the winters in 2012–2013. The field experiment was conducted in a randomized complete block design with split plots, having three replications. Fertilizer treatments (control, composted manure Higo Organic Plus at 5 t ha^?1, Maxicrop Sea Gold seaweed extract at 5 L ha^?1, farm yard manure at 10 t ha^?1, inorganic nitrogen–phosphorus (NP) at 90:60 kg ha^?1, NP at 120:90 kg ha^?1 and NP at 150:120 kg ha^?1) were allotted to main plots, while genotypes (Sandrina, Serenada and Kawe Terma) were allotted to the sub-plots. Plots treated with the application of NP at 120:90 kg ha^?1 produced the highest beet yield (76.4 t ha^?1) and sugar yield (11.1 t ha^?1), and had the second highest polarizable sugar content (14.52%) and more economic return (Rs. 553,000 per hectare) as compared to control plots. Sugar beet genotype Serenada had significantly higher beet yield (55.5 t ha^?1) and sugar yield (7.9 t ha^?1) and a higher economic return (Rs. 380,000 per hectare) than the other genotypes. Sugar beet genotype Serenada supplied with NP at 120:90 kg ha^?1is recommended for the general cultivation in the agro-climatic conditions of Peshawar valley.     

Effects of ambient gaseous pollutants on photosynthesis,growth, yield and grain quality of selected crops grown at different sites varying in pollution levels

In recent decades, ambient gaseous pollution has increased due to anthropogenic activities worldwide. The studies to evaluate the adverse effects of ambient pollutants on commonly grown food crops are still limited, especially in Asian countries like Pakistan. The present study was conducted to measure the ambient pollutants in different sites of Faisalabad and their impact on growth and yield of wheat, mung bean and peas. Plants were grown in pots and placed at three sites named as control (Wire house of Government College University, Faisalabad), low pollution (LP) (Farm Area of Ayub Agricultural Research Institute) and high pollution (HP) (GT Chowk, Faisalabad) sites. Results showed that ambient ozone (O₃) concentration was highest at HP site followed by LP site and was below AOT40 in control site. Ambient pollutants caused foliar injury in crops and decreased plant height, leaf area, biomass and grain yield. Pollutants caused a reduction in photosynthetic pigments, stomatal conductance and net photosynthetic rate and grain protein contents in all three crops. In conclusion, the ambient O₃ level was highest at HP site, this current O₃ level and other pollutants decreased the growth and yield of important food crops.     

Role of active aluminum in the formation of water-stable macroaggregates

Soil structure is determined by the arrangement of particles in soil and the particles of sand, silt, and clay bind together into aggregates of various sizes by organic and inorganic materials. Structural stability which is the ability of the aggregates and pores to remain intact when subjected to stress, markedly affects crop production and soil erosion (Tisdall 1996). Since water, either directly as rainfall or as surface runoff is the main agent of aggregate breakdown, in the analyzes of stable soil aggregation, the term water-stable aggregation is generally used (Lynch and Bragg 1985). Water-stable aggregates have been divided into micro aggregates < 0.25 mm dia.) and macro aggregates (> 0.25 mm dia.) (Edwards and Bremner 1967; Tisdall and Oades 1982). Microaggregates show a relatively high stability against physical disruption (Edwards and Bremner 1967). On the other hand, macro aggregates are sensitive to soil management (Tisdall and Oades 1982).

There are many reports on the relationships between the aggregate stability and the soil physicochemical properties. For example, significant correlations were found between the aggregate stability and the amounts of organic C (Tisdall and Oades 1982), total N, and carbohydrates or the CEC (Chaney and Swift 1984). However, most of these studies were conducted in non-volcanic ash soils. Volcanic ash soils are widely distributed in Japan and are very important soils for crop production. The objective of this study was, therefore, to obtain more information on the relationship between the degree of macro aggregation and the soil physicochemical properties in non-volcanic and volcanic ash soils.     

Boron-Calcium Synergically Alleviates Aluminum Toxicity in Wheat Plants (Triticum aestivum L.)

The effects of B and Ca treatments on root growth, nutrient localization and cell wall properties in wheat ( Triticum aestivum L.) plants with and without Al stress were investigated. Seedlings were grown hydroponically in a complete nutrient solution for 7 d and then treated with B (0, 40 μM), Ca (0, 2,500 μM), and Al (0, 100 μM) in a 500 μM CaCl₂ solution for 8 d. The cell wall materials (CWM) were extracted with a phenol: acetic acid: water (2:1:1 w/v/v) solution and used for subsequent pectin extraction with trans -1,2-diami-nocyclohexane- N,N,N,N -tetraacetic acid (CDTA) and Na₂CO₃ solutions. Boron, Ca, and B + Ca treatments enhanced root growth by 19.5, 15.2, and 27.2%, respectively, compared to the control (pH 4.5). Calcium and B+Ca treatments enhanced root growth with Al stress by 43 and 54%, respectively, while B did not exert any effect. The amounts of CWM and pectin per unit of root fresh weight increased by Al treatment, whereas the Ca and B+Ca treatments slightly reduced the contents of these components. Seventy-four percent of total B, 69% of total Ca, and 85% of total Al were located in the cell wall in the B, Ca, and Al treatments, respectively and 32% of total B, 33% of total Ca, and 33% of total Al were located in the CDTA-soluble and Na₂CO₃-soluble pectin fractions. A more conspicuous localization of B was observed in the presence of Al. Aluminum treatment markedly decreased the Ca content in the cell wall as well as pectin fractions, mainly in the case of the CDTA-soluble pectin fraction. Boron + Ca treatment decreased the Al content in the cell wall and pectin fractions compared to the Ca treatment alone in the presence of Al. It is concluded that the B+Ca treatment enhanced root growth and, B and Ca uptake, and helped to maintain a normal B and Ca metabolism in the cell walls even in the presence of Al.     

Genetic tracking of the brown bear in northern Pakistan and implications for conservation

Asian bears face major threats due to the impact of human activities as well as a critical lack of knowledge about their status, distribution and needs for survival. Once abundant in northern Pakistan, the Himalayan brown bear (Ursus arctos isabellinus) has been exterminated in most of its former distribution range. It presently occurs sparsely, in small populations, the Deosai National Park supporting the largest isolate. This decline might imply a reduction in genetic diversity, compromising the survival of the population. Using a combination of fecal DNA analysis and field data, our study aimed at assessing the size and genetic status of the Deosai population and give guidelines for its conservation and management. Using fecal genetic analysis, we estimated the population to be 40-50 bears, which compares well with the field census of 38 bears. The northern Pakistani brown bear population may have undergone an approximate 200-300-fold decrease during the last thousand years, probably due to glaciations and the influence of growing human population. However, in spite of the presence of a bottleneck genetic signature, the Deosai population has a moderate level of genetic diversity and is not at immediate risk of inbreeding depression. Gene flow might exist with adjacent populations. We recommend careful monitoring of this population in the future both with field observations and genetic analyses, including sampling of adjacent populations to assess incoming gene flow. The connectivity with adjacent populations in Pakistan and India will be of prime importance for the long-term survival of Deosai bears.