小麦叶锈菌生理小种MFR的分子鉴定研究

 用AFLP方法对来自中国和墨西哥的23个小麦叶锈菌生理小种进行分析,共筛选了64对引物,获得一对引物(M₀₅/E₀₃)可在MFR小种中扩增出一条特异性DNA片段,进行回收、克隆、测序,结果表明该片段具有325个碱基。根据特异性片段序列设计出SCAR标记引物,对60个叶锈菌生理小种分离物进行回检结果表明,研制的SCAR标记能够准确区分MFR生理小种。本实验结果为小麦锈菌生理小种分子检测体系的建立奠定了基础     

禾谷镰孢菌对多菌灵的敏感性基线及抗药性菌株生物学性质研究

 引起小麦赤霉病的禾谷镰孢菌(Fusarium graminearum)在华东地区对多菌灵已出现了高水平抗药性。本文报道了F.graminearum对多菌灵的敏感性基线及其抗药性菌株生物学特性。离体条件下多菌灵对100个野生敏感型菌株的平均EC₅₀和MIC值分别为0.5748±0.0133 μg/ml和 < 1.4μg/ml。而对50个抗药性菌株的平均EC₅₀和MIC值则分别为9.2375μg/ml和 > 100 μg/ml。在麦穗上多菌灵对50个敏感菌株和抗药性菌株防效的平均EC₅₀分别为282.6 μg/ml和 > 2 000μg/ml。从田间获得的抗多菌灵菌株对苯菌灵、噻菌灵、甲基托布津表现交互抗药性,但不同于室内突变菌株,对乙霉威不表现负交互抗药性。抗药性菌株的无性和有性繁殖后代以及在无药培养基上菌丝体转代培养后,抗药水平保持不变。抗药性菌株的菌丝生长速率、产孢能力及致病力等与敏感菌株相比没有差异。     

小麦白粉病菌闭囊壳萌发及其对三唑酮敏感基线的建立

在没有甾醇脱甲基抑制剂 (DMI)类药剂用药历史的湖北利川山区麦田采集小麦白粉病菌闭囊壳 ,带回室内萌发 ,从每样本单孢子堆中分离得到61个野生菌株。采用叶段法测得每个菌株的EC₅₀,建立起小麦白粉病菌对三唑酮的相对敏感基线EC₅₀=(0.1090±0.0048)μg/ml。     

system modeling and simulation of wind turbines

Wind turbines operate in harsh environment with transient and variable working conditions. As a result, wind turbines are complicated, nonlinear, uncertain systems. Modeling and analysis of these systems are crucial to their design. Based on MATLAB, a new dynamic simulation model of a stalled wind turbine was presented. The blade element momentum theory and the state space method were programmed into MATLAB/SIMULINK to calculate wind turbine aerodynamic performance. The generator was also modeled in MATLAB/SIMULINK, taking the coupling between the generator and the rotor speed into consideration. Based on the proposed MATLAB model, an analysis of a 600 kW wind turbine was carried out. The calculation values were compared with the measured data. The results indicate the correctness of the proposed MATLAB model. The simulation model can be applied to optimize design and control of stalled wind turbines.     

猪瘟病毒E2基因抗原结构域A、B、C、D区在大肠杆菌中的表达

采用PCR技术扩增出猪瘟病毒E2基因抗原结构域A、B、C、D区并克隆于PGEM-T easy载体上，测序结果表明插入的为猪瘟病毒E2基因抗原结构域，切出目的基因，将目的基因克隆到表达质粒pProEX—HTb中，获得重组质粒经PCR、酶切和序列分析鉴定E2基因抗原结构域插入的位置、大小和读码框均正确，SDS—PAGE检测表明受体菌经IPTG诱导后能表达E2基因抗原结构域蛋白，Westernblot检测表明受体菌诱导表达E2基因抗原结构域蛋白可与猪瘟阳性血清发生特异性反应。     

Comparison of Strength and Ultimate Strain Models of Concrete Columns Confined with FRP

Based on maintaining the accuracy of existing models for circular specimens, revised models were proposed for predicting strength and ultimate strain of concrete columns confined with FRP with considering the effectiveness of confinement by cross section. An evaluation of existing strength and ultimate strain models of concrete columns confined with FRP was presented with extensive collection of experimental data. It was shown that the prediction of strength was more accurate than that of ultimate strain, among which the prediction of Gampione’s strength model and Lorenzis’s ultimate strain model were more accurate for all specimens and circular specimens, respectively. The comparison with experimental results and existing models showed that the proposed models were more accurate, simpler and more convenient. The proposed models can be used for the prediction of strength and ductility in practical engineering.     

基于名师工作室的高职畜牧兽医专业教学改革与实践

本文以职业教育省级名师工作室建设为背景,围绕畜牧兽医专业人才培养模式、教师教学团队、优质课程资源、就业创业意识及社会服务等方面开展研究与实践探索,以期为工作室建设提供优化路径及理论依据。     

阿魏蘑与杏鲍菇高产优质栽培模式研究

试验证明，阿魏蘑和杏鲍菇最佳栽培模式为菌棒半脱袋竖向畦栽半覆土栽培模式；最适环境条件为菇房温度13～20℃，空气相对湿度85％～95％；最佳栽培设施为半地下冬暖式塑料大棚。     

丁硫克百威在韭菜和土壤中的残留动态研究

通过大田试验及气相色谱分析,研究了丁硫克百威在韭菜和土壤中的残留动态。结果表明,丁硫克百威在韭菜中的残留半衰期为1.5d,在土壤中,未能检测出丁硫克百威残留。按推荐剂量750mL·hm-2和2倍剂量1500mL·hm-2施用丁硫克百威20%乳油,使用3次,采样距最后一次施药间隔期14d时,丁硫克百威及其代谢物在韭菜和土壤中的残留量分别为0.026mg·kg-1、未检出和0.097mg·kg-1、未检出。