不同驯食方式对鳜鱼胃肠道消化酶活性的影响

在水族箱中,对用建立摄食条件反射过渡法驯化和饥饿后直接驯化法驯化的鳜鱼胃肠道中消化酶活性的变化,进行了比较测定．结果表明：胃蛋白酶和类胰蛋白酶的活性随投饲和摄食活动而变化．投饲后,鳜鱼体内胃蛋白酶和类胰蛋白酶活性缓慢上升;而摄食后,食物进入消化道,胃蛋白酶和类胰蛋白酶活性则迅速升高．在摄食条件下,消化道类胰蛋白酶的活性高于胃蛋白酶．条件反射组的类胰蛋白酶活性、驯化率和生长比速均显著高于直接驯化组,从而说明采用条件反射法驯化比较符合鳜鱼的生理变化规律     

冷水处理对水稻温敏核不育系育性影响的初步研究

报道了不同临界温度温敏核不育系的育性对冷水处理的反应，证实适当温度的冷水处理具有恢复育性的效果，并讨论了冷水处理、材料耐冷性及冷水处理的应用等问题     

海南4个地区暹罗炭疽菌群体遗传结构分析

对分离自海南4个地区13种寄主植物上暹罗炭疽菌的ITS-CAL-GAPDH 3基因序列进行群体遗传结构分析。95条暹罗炭疽菌多基因序列可定义为16个单倍型,其中,单倍型H5为主要单倍型,分布于所有寄主植物。AMOVA分析显示,遗传变异主要发生在种群内;病菌未经历过大规模的种群扩张过程。研究结果表明,暹罗炭疽菌种群具有丰富的遗传多样性。     

“农超对接”模式下的供应链管理研究——基于农产品流通的视角

国家商务部、财政部、农业部于2009年在全国12个省区共同启动的农产品流通模式改革,将“农超对接”工作列为发展现代流通、建设现代农业的重要任务.基于这个视角,拟通过对建立合理、高效的农产品供应链模式的研究,以期达到在“农超对接”中减少农产品流通环节、降低其流通成本及损耗,并最终实现增加农民收入、稳定城镇农产品价格、促进城乡协调发展的目的.     

Cloning and Expression of Gene Responsible for High-Tillering Dwarf Phenotype in Indica Rice Mutant gsor23

High-tillering dwarf mutant gsor23 was generated from an indica rice variety Indica9 radiatied by γ-ray. Genetic analysis showed that this phenotype was controlled by one single recessive gene, which was mapped within a physical distance of 386 kb between two insertion-deletion (InDel) markers C1-WT2 and C1-WT4 on the long arm of chromosome 1. There is a known gene D10 within this region, the mutation of which causes high-tillering in rice. Sequence analysis of the D10 allele in gsor23 revealed that the base cytosine (C) at the 404^th position in the coding region was deleted, which would cause frameshift mutation after the 134^th amino acids. The mutation site and indica background of gsor23 were different from the previously reported japonica mutants d10-1 and d10-2. Therefore, gsor23 is a novel allelic mutant of D10 which encodes the carotenoid-cleaving dioxygenase 8 (CCD8), a key enzyme involved in the biosynthesis of the new plant hormone strigolactones (SLs). After treatment with GR24, a synthetic analogue of SLs, the high-tillering phenotype of gsor23 was restored to normal. Real-time RT-PCR analysis showed that D10 expression was high in roots, but low in leaves. Compared with the wild type Indica9, the expression of the SL biosynthesis gene D10 was upregulated, while genes likely involved in the SL signal transduction pathway such as D3 and D14 were down-regulated in the gsor23 mutant.     

浅析农民工转移培训与农广校发展

概括分析安徽省安庆市农村劳务输出情况及当前劳务输出所面临的困难与问题,指出农广校开展农民工转移培训势在必行,并提出其对策与途径。     

中华绒螯蟹配合饲料中蛋白质、脂肪、纤维素的适宜含量↑（*）

试验采用正交L18(37)表,用酪蛋白、结晶纤维素、鱼油分别作为蛋白源、纤维素源和脂肪源,制成含蛋白质36%、40%、44%,脂肪4%、6%、8%,纤维素3%、5%、7%的试验饲料,在水族箱中对体重19～25g的中华绒螯蟹(Eriocheirsinensis)配合饲料中蛋白质、脂肪、纤维素的适宜含量进行研究。结果表明,配合饲料中含40%～44%蛋白质、4%～6%脂肪、3%纤维素具有显著的促增重效果,高的生长比率,良好的饲料系数和蛋白质效率(P<0.01),交互作用极显著(P<0.01);5%和7%纤维素都显著地降低了生长比率、蛋白质效率、蛋白质表观消化率,使饲料系数极显著升高(P<0.01)。     

Isolation of infectious microalga Prototheca wickerhamii from a carp (Cyprinus carpio) – a first confirmed case report of protothecosis in a fish

Protothecosis is a rare infection caused by environmentally ubiquitous achlorophyllic microalgae of the genus Prototheca. Here, we describe a first case of protothecosis in a carp (Cyprinus carpio), which is at the same time the first case of protothecosis in a fish, confirmed by phenotype‐ and molecular‐based methods, including PCR sequencing of the rDNA cluster and protein profiling using matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry.     

饲料脂肪源和碘酸钾对中华鳖稚鳖生长、存活率、肝肠组织形态和脂类代谢的影响

为了比较饲料中添加鱼油、茶籽油、亚麻籽油以及碘酸钾对中华鳖（）稚鳖生长和脂类代谢的影响,本研究配制了4种油脂含量为10%的饲料,分别为鱼油料（FO,对照组）、鱼油+碘酸钾料（FO+PI,碘酸钾添加量为75 mg/kg）、茶籽油料（TO）和亚麻籽油料（LO）,饲喂初重为（5.06±0.05）g的中华鳖66 d。FO+PI组和LO组中华鳖稚鳖的存活率显著低于FO组（<0.05）。各组中华鳖稚鳖血浆中葡萄糖、总胆固醇和甘油三酯水平无显著差异（ACACA）的表达水平,上调了中华鳖-499的表达水平（-23b的表达水平（<0.05）。FO+PI组和TO组的稚鳖肝脏细胞内脂滴空泡较少,同时TO显著影响了肠道组织的黏膜褶。结论认为,相比于鱼油组,在饲料中添加10%茶籽油、亚麻籽油和添加75 mg/kg碘酸钾不会引起稚鳖生长差异,但是亚麻籽油和碘酸钾降低了稚鳖的存活率,茶籽油和碘酸钾影响肝脏的脂类代谢。