Technical note: recombinant LHRH fusion protein suppresses estrus in heifers

A recombinant luteinizing hormone-releasing hormone (LHRH) fusion protein was evaluated for its effectiveness in suppression of estrus in heifers. Eight heifers were randomly assigned to two equal treatment groups. Treatments consisted of recombinant ovalbumin-LHRH-7 or recombinant ovalbumin (control). This recombinant chimeric fusion protein consisted of ovalbumin with seven LHRH peptides (ovalbumin-LHRH-7). The plasmid for this protein was expressed in E. coli and was collected and purified as an insoluble protein. One milligram of the respective proteins was suspended in 2 mL of Z-Max adjuvant and administered by intramammary injection three times at 7-wk intervals. Luteinizing hormone-releasing hormone antibody binding was elevated in heifers treated with ovalbumin-LHRH-7 compared to ovalbumin-treated heifers (P < .05). Serum progesterone concentrations (< 1 ng/mL) indicate that the estrous cycle of the four heifers treated with ovalbumin-LHRH-7 was suppressed for a time period ranging from 60 to 238 d, which was different from control heifers (P < .01). Serum progesterone for the control heifers continued to exhibit cyclic profiles over the experimental period. This preliminary study in heifers demonstrated that a chimeric LHRH fusion protein induced elevated concentrations of circulating LHRH antibodies that suppressed estrus for an average of 122 +/- 41 d.     

乳牛口服大量β-胡萝卜素对维生素A的吸收及钙磷代谢的影响

从北京西郊某农场选取６头健康成年黑白花乳牛,随机分成试验和对照两组。整个试验周期共８周,分为前期,中期和后期。试验结果表明,试验组乳牛血清β－胡萝卜素水平与血清ＡＫＰ活性的变化规律相一致,均于中期时最高,血清ＡＫＰ同工酶酶谱中骨带染色变宽变深;血清维生素Ａ水平呈现逐渐升高趋势;血清钙水平呈下降趋势;血清无机磷水平逐渐下降。据此可以表明,β－胡萝卜素进入机体后,其转化为维生素Ａ的效率在不同的吸收阶段     

用重组核衣壳蛋白ELISA检测PRRSV抗体的研究

ＰＲＲＳＶ重组Ｎ蛋白ＥＬＩＳＡ检测人工感染猪血清７５份,与ＩＤＥＸＸ公司ＥＬＩＳＡ试剂盒及ＩＦＡ的符合率分别为９７．３％和１００％。检测疫苗免疫猪血清,阳性率１００％。在免疫后６ｄ就能检出抗体,比ＩＦＡ敏感。检测８５份我国田间猪血清,阳性率１８．８％。     

低成本省力化栽桑技术的研究进展

我国已育成了若干适合条桑育,适于扦插的桑品种及适于种子直播的杂交桑,为建立高密度低成本桑园打下了基础。推广了直接用袋接体,硬枝扦插和杂交种直接建园和初试了中晚秋条桑收获技术。今后的研究方向是选育发根性强,高产优势,适合条桑收获的桑树品种及以条桑收获为主的提高劳动生产率的栽培技术。     

秋冬交替季节鸡常见病的防治

一鸡传染性鼻炎1病原及流行特点本病是由副鸡嗜血杆菌引起的一种鸡的急性上呼吸道传染病。副鸡嗜血杆菌属于革兰氏阴性菌,有A、B、C三个血清型。前几年,我国流行的血清型主要是A型和零星C型,但近年来主要血清型是A和B型,且A型已经出现变异,发病比例有明显增加的趋势。     

Regulation of fibroblast growth factor 9 on the differentiation of goat intramuscular adipocytes

It has been found that fibroblast growth factor receptor (FGF-FGFR) signaling can regulate the expression of adipocyte differentiation genes. FGF9 is one of the members of FGFs that mainly binds receptors FGFR2 and FGFR3. FGF9 is highly expressed in the adipose tissue of humans and mice, but there are few reports on the role of FGF9 in goat intramuscular adipocyte differentiation. Therefore, this study explored the effect of FGF9 on adipocyte differentiation through cell culture, interference, and overexpression. The expression of receptors FGFR1–FGFR4 in adipocyte differentiation and their effects on differentiation were detected to screen receptor gene of FGF9. Finally, the interaction between FGF9 and the receptor was tested by cotransfection. Our results showed that FGF9 interacts with FGFR2 to inhibit goat intramuscular adipocyte differentiation by regulating peroxisome proliferator-activated receptor gamma (PPARγ) and preadipocyte factor 1 (Pref1), which is a data support for subsequent pathway research.     

秦川牛保种情况的分析 Ⅰ群体规模、年龄结构与迁移

采用群体遗传学理论与方法研究了陕西省秦川牛场秦川牛保种群30多年来的保种情况,详细地分析了保种群的规模、年龄结构及迁移情况。研究发现,该保种群规模变化剧烈,总的来说规模偏小,尤其是公牛群远小于保种遗传学基本法则规定的最小值;畜群结构不稳定,迁入不规则,该研究结果将为今后采取适当的措施提高保种效果提供科学的参考依据。     

基于文献统计分析桑树研究的进展

通过对国内外各大数据库中桑树相关文献量的计量,对桑树的研究状况进行了分析。结果显示,桑叶作为家蚕饲料的传统农业模式已被打破,现已转向涉及医药、食品与保健等领域的多元化发展的桑树资源综合开发利用模式。其中,对桑树活性物质的研究由生物碱类物质转向多酚类物质和多糖类物质,桑树的抑制肿瘤和抗癌活性逐渐被重视,国内研究者对桑树的研究深度及影响力逐年提高。该结果可为桑树资源的进一步研究提供一定的参考。     

肠黏膜自然杀伤细胞的免疫功能及其调控

自然杀伤(NK)细胞是肠黏膜免疫屏障的重要组分。本文从NK细胞的发育学特征、分子表型切入,分析了其在维护肠道屏障完整性中发挥的功能,以及肠道微生物对其免疫功能的调节作用,旨在为疾病预防和肠道健康的营养调控提供理论依据。     

温度、光照和发芽床对假俭草种子萌发的影响

实验室内进行了假俭草种子净度检验样品最低限量和发芽检验方法的研究，探讨了光照（以黑暗为对照）、温度（分别包括２０℃恒温，１５／２５℃、２０／３０℃、２０／３５℃和２５／３５℃变温）和发芽床（分设纸上ＴＰ和砂中Ｓ）等因素对种子发芽的影响。结果表明：假俭草种子净度检验样品最低限量是３ｇ，送验样最低量以及计数其它植物种子检验样品最低量均为３０ｇ；最佳萌发条件为２０／３０℃或２０／３５℃变温（１６小时低温，８小时高温），高温时段设光照，和纸上（ＴＰ）发芽；发芽的初次和未次计数时间分别以第１０天和第２１天为宜；种子的幼苗发育可划分为单子叶植物子叶留土类型。