mRNA差异显示技术及其在动物遗传育种中的应用

ｍＲＮＡ差异显示技术是近年来国外发展起来的攻克隆新基因的一种新方法,同时已广泛用于人类基因调控和克隆研究。本文论述了这一新技术的原理、发展现状和在动物遗传育种中的应用领域。     

犬严重子宫蓄脓的影象学诊断

子宫蓄脓本为犬多发的常见病,诊断并不困难。最近我们收治１例特别严重的子宫蓄脓,诊断一时难以明确,经综合影象学检查后,方得以确定,报告如下。１　病史华南农业大学动物医学系动物医院收治１例本地母犬,７岁,从未配种怀孕。因不食、腹围渐胀大而求诊,临床疑为腹水症,两天前　　收稿日期：１９９８１１２７抽出过少量液体。２　体格检查病犬精神差,躺卧不能站立和运动,稍消瘦和贫血,厌食已２０余天,每天只饮水,无大便,尿量正常;心跳快而心音弱,脉搏１５０次／ｍｉｎ,呼吸５６次／ｍｉｎ,体温３９２℃。就诊时饮水…     

DIC and granulomatous vasculitis in a dog with disseminated histoplasmosis

Disseminated histoplasmosis and disseminated intravascular coagulopathy were diagnosed in a 7 mo old, female spayed mixed-breed dog. The dog improved transiently with supportive care, but deteriorated shortly after initiation of antifungal therapy. The dog was subsequently euthanized. At necropsy, marked granulomatous vasculitis was identified in all affected organs. The tunicae and laminae of the arteries and arterioles were obscured by epithelioid macrophages and multinucleated giant cells admixed with necrotic material. Intracytoplasmic yeast were present within some of these macrophages. To the authors' knowledge, this is the first reported case of granulomatous vasculitis associated with Histoplasma capsulatum in a dog.     

Genetic diversity and relatedness of Fasciola spp. isolates from different hosts and geographic regions revealed by analysis of mitochondrial DNA sequences

The present study examined sequence variability in a portion of the mitochondrial cytochrome c oxidase subunit 1 (pcox1) and NADH dehydrogenase subunits 4 and 5 (pnad4 and pnad5) among 39 isolates of Fasciola spp., from different hosts from China, Niger, France, the United States of America, and Spain; and their phylogenetic relationships were re-constructed. Intra-species sequence variations were 0.0-1.1% for pcox1, 0.0-2.7% for pnad4, and 0.0-3.3% for pnad5 for Fasciola hepatica; 0.0-1.8% for pcox1, 0.0-2.5% for pnad4, and 0.0-4.2% for pnad5 for Fasciola gigantica, and 0.0-0.9% for pcox1, 0.0-0.2% for pnad4, and 0.0-1.1% for pnad5 for the intermediate Fasciola form. Whereas, nucleotide differences were 2.1-2.7% for pcox1, 3.1-3.3% for pnad4, and 4.2-4.8% for pnad5 between F. hepatica and F. gigantica; were 1.3-1.5% for pcox1, 2.1-2.9% for pnad4, 3.1-3.4% for pnad5 between F. hepatica and the intermediate form; and were 0.9-1.1% for pcox1, 1.4-1.8% for pnad4, 2.2-2.4% for pnad5 between F. gigantica and the intermediate form. Phylogenetic analysis based on the combined sequences of pcox1, pnad4 and pnad5 revealed distinct groupings of isolates of F. hepatica, F. gigantica, or the intermediate Fasciola form irrespective of their origin, demonstrating the usefulness of the mtDNA sequences for the delineation of Fasciola species, and reinforcing the genetic evidence for the existence of the intermediate Fasciola form.     

冷季藏系羊补饲的效果观察

通过对20头2岁母藏系羊补饲,在补饲期间期中增重3.32kg,增重率为14.46%。期末增重0.91kg,增重率为3.81%。试验组羊总增重4.23kg,增重率为17.20%。对照组羊在期中增重-0.69 kg,增重率为-3.51%。对照组羊总增重-3.36kg,总增重率为-19.83%。补饲结束后在6月份增重2.65kg,增重率为9.99%。7月份增重8.03kg,增重率为23.24%。8月份增重5.18kg,增重率为13.04%。9月份增重8.73kg,增重率为17.42%。夏秋总增重21.59kg,增重率为44.88%。对照组羊在6月份增重0.29kg,增重率为1.70%。7月份增重3.74kg,增重率为17.92%。8月份增重2.13kg,增重率为9.26%。9月份增重6.22kg,增重率为21.22%。对照组羊在夏秋草场总增重12.09kg,增重率为41.88%。     

关于生物安全隔离区建设试点的几点思考

动物疫病区划管理是当前国际通行的动物疫病管理措施,目的是实现动物疫病控制或净化,提高动物卫生水平,促进动物、动物产品的国际贸易。2003年,世界动物卫生组织(OIE)提出了动物疫病区域化管理新模式--生物安全隔离区划,即在同一生物安全管理系统下,通过采取监测、控制和生物安全管理措施,建立并维持无一种或多种特定动物疫病状态的无疫生物安全隔离区。虽然该概念提出时间较短,但已引起了全世界范围的广泛关注,欧盟和澳大利亚、泰国等一些畜牧业发达国家都在进行积极地研究和探索。我国也在无疫示范区建设基础上,根据OIE有关标准和建议,研究制定生物安全隔离区建设和评价标准,并着手开展试点工作。     

胸膜莫肺炎放线杆菌APXIV部分基因片段的表达及纯化

参照GenBank中已发表的ApxIV基因序列,以自行分离的App DNA为模板,利用PCR方法扩增出ApxIV3'端,大小为552bp的保守基因序列.将PCR产物克隆到pMD18-T Simple Vector中,获得重组质粒pMD-ApxIV,对其重组质粒pMD-ApxIV进行BamHI、HindIII双酶切,并将酶切产物克隆到原核表达载体pET-32a(+)中,构建了重组表达质粒pET-ApxIV.将表达质粒转化至大肠杆菌BL21中,用IPTG诱导表达,通过SDS-PAGE和Western blot分析,结果表明pET-ApxIV在BL21中成功表达,并能被App阳性血清所识别,具有良好的免疫原性.表达蛋白的分子质量约为39.5KDa.利用HiTrap FF crude columns将表达的蛋白进行了纯化.     

辉煌后涅槃 涅槃中轮回——2009豆市年度投资报告

经过2008年全球金融危机引爆的资本市场“去杠杆化”后．全球大豆市场走势将逐步回归供需面的引导。由于全球走出经济危机尚需时日,2009年国内外大豆期价很难走出单边行情．预期将更倾向于宽幅区间振荡行情。如果2009年美豆种植面积减少．2009年下半年经济好转,流动性再度充裕．那么2009／10年度美豆的农场价格有可能高于本年度。但年度均价在未来的1到2个年度仍倾向于下滑。     

Adjuvant enhancement of humoral immune response to chemically inactivated bovine viral diarrhea virus.

Potentiation of the antibody response to inactivated bovine viral diarrhea virus by immunological adjuvants was studied in guinea pigs and cattle. The inactivated bovine viral diarrhea virus alone was demonstrated to be a weak immunogen. Addition of either 2 mg per mL diethylaminoethyl-dextran or 5% alhydrogel to inactivated bovine viral diarrhea virus did not or only slightly stimulated the antibody response; the combined adjuvants induced a significantly higher titer. A higher concentration (20 mg per mL) of diethylaminoethyl-dextran, on the contrary, suppressed the immune potentiation by the dual adjuvants. Combination of Bordetella bronchiseptica and alhydrogel adjuvants stimulated a high titer of antibody. The titer was further elevated upon revaccination and was significantly higher than that induced by alhydrogel alone. In cattle, alhydrogel enhanced the immune response and the additional inclusion of diethylaminoethyl-dextran did not cause a significant potentiation of the immunity. However, the antibody decay rate was significantly slower when stimulated by the combined adjuvants.     

安徽省一起非洲猪瘟疫情的暴发调查

2018年9月,安徽省铜陵市某猪场暴发非洲猪瘟疫情。为查明疫情可能的来源、波及范围,继而提出有针对性的预防控制建议,对该猪场的饲养管理、猪群发病现状以及相关流行病学关联场点进行了现场调查。调查发现,此次暴发可能由饲喂泔水引起。由此建议:暂时关闭辖区内生猪屠宰场,并进行彻底消毒;停止疫情相关区域生猪及其产品调运;监测受威胁区猪场,做好生物安全防护;停止饲喂泔水,并做好餐厨剩余物的无害化处理工作。