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301.
302.
Effect of Bacillus cereus as a water or feed additive on the gut microbiota and immunological parameters of Nile tilapia
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Miao Wang Guanbin Liu Maixin Lu Xiaoli Ke Zhigang Liu Fengying Gao Jianmeng Cao Huaping Zhu Mengmeng Yi Deguang Yu 《Aquaculture Research》2017,48(6):3163-3173
We evaluated the effects of Bacillus cereus, as an additive in water and feed, on the gut microbiota and immunological parameters of Nile tilapia (Oreochromis niloticus) fingerlings. Experiments were performed in tanks and net cages respectively. Experiment 1: Tilapia were housed in tanks for 42 days, and B. cereus was added to the water at 1.0 × 104 cfu mL?1 (Treatment 1) and 1.0 ×105 cfu mL?1 (Treatment 2) weekly. For the control, no probiotic was added. Experiment 2: Tilapia were housed in cages for 42 days, and the feed was supplemented with B. cereus at 1.0 × 107 cfu g?1 (Treatment 1) and 1.0 × 108 cfu g?1 (Treatment 2) weekly. For the control, no probiotic was added. Each treatment contained three replicates, with 50 male tilapias per replicate. The fish from the probiotic treatments in both tank and cage experiments had significantly higher serum lysozyme and peroxidase activities than the control. In the cage experiment, alkaline phosphatase and total superoxide dismutase activities in tilapia were significantly higher in probiotic treatments compared with the control. The results of polymerase chain reaction‐denaturing gradient gel electrophoresis showed that B. cereus supplementation in the feed and water affected the autochthonous gut bacteria community of tilapia and stimulated various potentially beneficial bacteria. Therefore, B. cereus, as a water or feed additive, could enhance the immune status and affect the gut microbiota of tilapia. Bacillus cereus was more effective as a feed supplement rather than a water additive for enhancing the immune status of tilapia. 相似文献
303.
Comparative study on dietary protein requirements for juvenile and pre‐adult gibel carp (Carassius auratus gibelio var. CAS III)
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Two 8‐week growth trials were conducted in indoor recirculation system to evaluate the protein requirements for juvenile (3.70 ± 0.20 g) and pre‐adult (85.2 ± 0.70 g) gibel carp, Carassius auratus gibelio var. CAS III. Six isoenergetic diets were formulated for each trial using fish meal and casein as protein sources, and protein level was 250–450 g kg?1 in Trial 1 and 200–450 g kg?1 in Trial 2. With the increasing dietary protein, feeding rate (FR) and feed conversion ratio (FCR) significantly decreased (P < 0.05). Weight gain (WG) increased first and then reached a plateau in 330–450 g kg?1 in Trial 1 (P > 0.05), while decreased after the maximum value in 350 g kg?1 in Trial 2 (P < 0.05). Productive protein values (PPVs) were lower in 370–450 g kg?1 in Trial 1 and 400–450 g kg?1 in Trial 2 (P < 0.05). Increasing dietary protein level increased protein content and decreased lipid content in whole fish body and white muscle (P < 0.05). Apparent digestibility coefficient of dry matters (ADCd) decreased, while apparent digestibility coefficient of protein (ADCp) increased in 370–450 g kg?1 in Trial 1 and 250–450 g kg?1 in Trial 2 (P < 0.05). Trypsin activity significantly increased in 370–450 g kg?1 in Trial 1 (P < 0.05) and was not affected in Trial 2 (P > 0.05). Hepatic alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities in both trials increased when dietary protein was above 400 g kg?1 (P < 0.05). Based on quadratic regression of WG, it was estimated that dietary protein requirement for maximum growth was 414 g kg?1 (digestible protein of 376 g kg?1) and 365 g kg?1 (digestible protein of 324 g kg?1) for juvenile (3.70 g) and pre‐adult gibel carp (85.2 g). 相似文献
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A study was conducted to investigate the effect of dietary yeast polysaccharides on some hematologic parameters and intestinal morphology of channel catfish. Channel catfish were fed diets containing yeast polysaccharides at 0 (control), 0.1, 0.2, or 0.3?% for 7?weeks. Each diet was provided to 10 channel catfish specimens (5.82?±?0.13?g initial weight) replicated 3?times in individual 250?L fiberglass tanks. Some hematologic parameters, leukocyte phagocytic activity, and intestinal morphology were monitored. After 7?weeks of trial, 0.2?% yeast polysaccharides resulted in significantly higher (P?0.05) monocyte numbers. Furthermore, fish fed 0.2?% yeast polysaccharide diet had higher (P?0.05) phagocytic rate of leukocyte. And 0.3?% yeast polysaccharide enhanced (P?0.05) phagocytic index of leukocyte. Histological evaluation showed yeast polysaccharide supplementation increased the height of intestine fold (0.1, 0.2 and 0.3?%) and the thick of muscular layers (0.2?%) in intestine (P?0.05). In addition, 0.1 and 0.3?% yeast polysaccharide supplementation improved the number of goblet cells (P?0.05). The results of this trial indicate that yeast polysaccharides supplementation could affect blood monocytes, improve leukocytes phagocytic activity, and the development of intestine in channel catfish. 相似文献
307.
东海带鱼摄食习性、饵料基础及与渔场的关系 总被引:9,自引:1,他引:9
本文对东海主要渔场中带鱼Trichiurus lepturus Linnaeus的摄食习性、饵料浮游生物的群落结构及数量分布等进行了阐述和分析,并对其与渔场间的关系进行了探讨。研究结果表明,东海带鱼是一种以磷虾等甲壳动物和小型鱼类等为主食,并兼食头足类、毛颚动物等的杂食性鱼类。研究结果还阐明浮游动物生物量及磷虾等的数量分布同东海带鱼渔场的位置变化和鱼群移动的关系密切,其中冬、春季浙江南部近海渔场;夏、秋季长江口及海礁、舟山等渔场,其关系尤为明显。 相似文献
308.
309.
“苏蟹一号”F3代选育群体生物学特性分析 总被引:3,自引:0,他引:3
经过连续3代的群体选育,"苏蟹一号"F3代选育群体呈现长江水系河蟹特征,表现出优质、高产、抗逆等优良经济性状。F3代选育群体雌蟹呈现二元分化,平均体重为150g、120g,雄蟹平均体重150g。11月上旬的养殖河蟹雌性群体可食部分分别占体重的35.05%、36.78%,雄性群体为29.56%。雌蟹肥满度、成熟系数、性/肝指数分别为0.54、9.69%、155.47%,0.55、10.51%、166.58%;雄蟹分别为0.59、2.31%、41.48%。雌蟹可食部分蛋白质含量21.62%、20.85%,脂肪含量10.37%、8.43%、氨基酸含量19.07%,、19.83%;雄蟹分别为15.20%、7.05%、13.78%。 相似文献
310.
浅色黄姑鱼鳃结构及其呼吸面积的研究 总被引:4,自引:0,他引:4
应用扫描电镜技术对体重范围在4.75~35.51g的浅色黄姑鱼(Nibea coibor)鳃结构及鳃小片进行了观察与呼吸面积计算,研究体重、体长与呼吸面积之间的相关关系。结果表明,浅色黄姑鱼的平均相对呼吸面积为4.01±1.62cm^2·g^-1。体重与一侧鳃丝总数的相关性N=28.81+158.45lnW(R^2=0.9670,P〈0.01)较体长与一侧鳃丝总数的相关性N=17.31L^1.4542(R^2=0.8241,P〈0.01)更为显著。单位mm鳃小片的数目随着体重的不断增加显著减少,N=49.971W^-0.3909(P〈0.01)。单个鳃小片的面积随体重的增大而显著增加,a=0.3199W^0.2528(P〈0.01)。总呼吸面积随体重的增大而显著增加,A=11.559W^0.7109(P〈0.01)。相对呼吸面积随体重的增大而显著减小,RA=11.561W^-0.2904(P〈0.01)。 相似文献