Seroprevalence of chlamydial infection in dairy cattle in Guangzhou, southern China

Chlamydia spp. are obligate intracellular gram-negative bacteria that cause a wide range of significant diseases in humans and animals worldwide, resulting in significant economic losses. Chlamydial infection in cattle has been reported in many countries including China. However, there has been no survey of chlamydial infection of dairy cattle in Guangzhou, southern China. The objective of the present investigation was to examine the chlamydial seroprevalence in dairy cattle in Guangzhou, subtropical southern China by using an indirect hemagglutination assay (IHA). The overall seroprevalence of chlamydial infection in dairy cattle was 7.25% (29/400). Greater than or equal to eight-yr-old dairy cattle had the highest seroprevalence (10.34%), followed by those that were ≥ 6 years old or < 7 years old dairy cattle (10.20%), although there were no statistically significant differences among different groups (P > 0.05). Dairy cattle with 5 pregnancies had the highest seroprevalence (10.81%). These results indicate that chlamydial infection was present in dairy cattle in Guangzhou, subtropical southern China, and integrated strategies and measures should be executed to control and prevent chlamydial infection and disease outbreak in the study region.     

蜥蜴利什曼原虫在小鼠体内的分布及增殖研究

通过腹腔途径用体内含有荧光蛋白的蜥蜴利什曼原虫感染BALB/c小鼠,感染后采其脏器做冰冻切片,荧光染料染色后用荧光显微镜观察,并经PCR鉴定,结果显示蜥蜴利什曼原虫感染小鼠后,主要分布于心脏、肝脏、脾脏、肺脏、肾脏。另外,成功建立了利什曼原虫荧光定量PCR检测方法,并采用该方法检测感染蜥蜴利什曼原虫后BALB/c小鼠体内利什曼原虫的增殖情况,结果显示,感染13 d内,BALB/c小鼠体内蜥蜴利什曼原虫呈波浪状增殖。这一结果为研究蜥蜴利什曼原虫感染人和动物的致病机理和免疫方法及疫苗研制等方面提供了基础理论依据。     

蜂产品质量安全关键技术研究发展分析

我国蜂产品和其它食品相似，各种污染和问题来自于从“养殖场到餐桌”的不同的生产、加工、流通过程，从过去到现在相当一段时期，主要问题包括：化学性物质污染（兽药、农药、重金属污染）、病原微生物污染、生物毒素污染、其它问题（搀假造假、致敏物、添加剂）等，其中化学污染中的药物残留污染是主要问题。     

水貂犬瘟热病的诊治

2001年6月上旬以来,胶东地区部分水貂养殖场出现以双相热、眼结膜炎、脚趾水肿等为特征的疾病.根据流行病学、临床症状、病理剖检、实验室诊断确诊为水貂犬瘟热病,及时采取措施,控制了本病的发展.现将本病的诊断与治疗情况报告如下.     

猪链球菌2型四川分离株毒力基因的检测及其序列分析

根据猪链球菌2型的MRP、EPF和SLY基因设计合成了四对引物,对我们分离鉴定的2005四川分离株进行毒力因子检测,并对阳性产物测序比较。结果以分离株为模板,获得了与预期结果一致的867bp(MRP)、572bp(EF)的2个片段,以及SLY的长度为1502bp目的片段(外引物)和1443bp的目的片段(内引物)。说明分离株SSsc0501为MRP+EF+SLY+,属于具有3种主要毒力因子的强毒株。经对PCR阳性产物测序及序列分析,结果其与P1/7株的核苷酸序列完全一致,与1933株的同源性达99.7%,仅在128位、217位、744位、1380位、1386位的5个核苷酸发生突变。在氨基酸水平上SSsc0501株与1933株的同源性达99.6%,有2个氨基酸发生替代。     

磷与奶牛营养需要的研究进展

磷元素对动物生理功能的影响非常显著,尤其是对奶牛的影响,本文以奶牛为例,重点综述磷在牛机体的分布和功能、磷的代谢平衡、磷缺乏、磷的需求量、磷状态指标、磷补充方法及其效率等,以便为我国奶牛业的发展提供理论指导和技术性参考.     

猪繁殖与呼吸综合征、猪2型圆环病毒病二联基因疫苗的构建

将猪繁殖与呼吸综合征病毒(PRRSV)长春分离株的ORF5基因克隆至真核表达载体pIRES的CMV的启动子下游,并用猪二型圆环病毒的内蒙古分离株的ORF2基因替代pIRES的neo基因,构建真核表达质粒。通过West-ernblot以及IFA检测方法鉴定,所构建的重组质粒能在BHK细胞内进行有效的转录,并表达了目的蛋白。     

Comparative analysis of proteomic profiles between endometrial caruncular and intercaruncular areas in ewes during the peri-implantation period

The endometrium of sheep consists of plenty of raised aglandular areas called caruncular (C), and intensely glandular intercaruncular areas (IC). In order to better understand the endometrium involved mechanisms of implantation, we used LC-MS/MS technique to profile the proteome of ovine endometrial C areas and IC areas separately during the peri-implantation period, and then compared the proteomic profiles between these two areas. We successfully detected 1740 and 1813 proteins in C areas and IC areas respectively. By comparing the proteome of these two areas, we found 170 differentially expressed proteins (DEPs) (P < 0.05), functional bioinformatics analysis showed these DEPs were mainly involved in growth and remodeling of endometrial tissue, cell adhesion and protein transport, and so on. Our study, for the first time, provided a proteomic reference for elucidating the differences between C and IC areas, as an integrated function unit respectively, during the peri-implantation period. The results could help us to better understand the implantation in the ewes. In addition, we established a relatively detailed protein database of ovine endometrium, which provide a unique reference for further studies.     

棉籽饼粕生物脱毒的研究进展

棉籽饼粕是一种重要的蛋白质资源，但因其含有一定量棉酚，影响了利用效果。用适宜的方法脱除或钝化游离棉酚，对开发优质蛋白资源具有重大意义。笔者综述了棉籽饼粕中的有毒物质、目前的生物脱毒研究及其发展前景。     

亚急性铜、镉暴露对鲤肝脏Hsp60表达的影响

试验通过实时定量PCR和Western blot技术,检测重金属铜、镉单一及联合急性暴露后鲤肝脏中Hsp60基因和蛋白水平。鲤随机分为7组,两个Cu2+处理组(0.05 mg/l和0.1 mg/l),两个镉处理组(0.63 mg/l和1.26 mg/l),两个混合处理组(0.045 mg/l和0.09 mg/l)和对照组。结果表明铜、镉单一及联合急性暴露对鲤有毒性作用,经过96 h的暴露,除混合低浓度组之外,其余各组肝脏中Hsp60基因表达水平显著提高(P0.05)。受重金属暴露的影响,初期鲤肝脏中Hsp60蛋白水平下降,随着时间推移,蛋白水平逐渐上升,混合低浓度组显著高于对照组水平(P0.05)。