用Nested-PCR检测广西黄皮与九里香黄龙病病原

摘要：运用nested-PCR技术对广西黄皮和九里香进行柑桔黄龙病（Citrus Huanglongbing, HLB）检测,结果表明：从部分黄皮和九里香样品抽提的DNA中,可扩增出一条特异性的400 bp条带,说明广西种植的黄皮和九里香均可感染黄龙病;在柑桔黄龙病疫区,黄皮和九里香黄龙病感染率分别为35.0%和40.0%。     

家畜排泄物综合处理工艺的研究

对我国广大农村家畜排泄物常规处理存在的利弊进行了调查研究,最终揭示出厌氧发酵制沼气、好氧发酵制复混肥的一套符合我国可持续发展、创建和谐社会、节约型社会的家畜粪便处理的科学、实用方法。     

Association of a missense mutation in the luteinizing hormone/choriogonadotropin receptor gene （LHCGR） with superovulation traits in Chinese Holstein heifers

Background: Upon binding luteinizing hormone in the ovary, the luteinizing hormone/choriogonadotropin receptor (LHCGR) is necessary for follicular maturation and ovulation, as well as luteal function. We detected mutations in the LHCGR gene and evaluated their association with superovulation. Methods: Using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing, we examined polymorphisms in LHCGR and the genotypes associated with superovulation traits in 127 Chinese Holstein heifers. Results: A G/T polymorphism (ss52050737) in exon 11 was significantly associated with the total number of ova and the number of transferable embryos. Conclusions: LHCGR may be a new predictor for superovulation in Chinese Holstein heifers.     

养鸡八禁忌

一、禁与鸭鹅混养：有的农户为节省饲养场地，把鸡、鸭、鹅关在一起混养，这是不科学的。一是混养易造成疫病的互相传播，如鸭、鹅也能感染新城疫病毒，在一般情况下，病毒潜伏于体内，成为带毒，不表现任何症状。若与鸡混养在一起，极易将病毒传染给鸡，导致鸡发生新城疫。二是鸡鸭鹅混养在一起，会使饲料利用率降低，     

巴比妥类镇静剂的检测研究进展

本文对巴比妥类安眠镇静剂检测方法的研究进展进行了综述。重点讨论了样品前处理方法及各种衍生化方法,GC、GC-MS、HPLC、HPLC-MS等检测方法。     

马正常肢体血流图实验研究

本实验首次采用XLJ—8型桥式血流图仪描记了12匹健康马四肢血流图.结果表明,马肢体血流图描记法是可行的,同时提供了健康马肢体血流图8项主要指标的参考值。本实验的首试成功,为肢体血流图在兽医临床领域中广泛应用奠定了基础.     

Studies on the pathogenicity ofBabesia bovis in water buffaloes after cryopreservation and resuscitation

Tropical Animal Health and Production - Packed erythrocytes infected withBabesia bovis were mixed with an equal volume of 16% dimethyl sulphoxide (DMS0) in Alsever’s solution and dispensed...     

LFA—3和IL—2对机体免疫增强作用

以绵羊红细胞膜提取的ＬＦＡ－３和猪外周血单个核细胞所产生的ＩＬ－２，同时或分别与新城疫Ⅳ系疫苗胸肌注射２２日龄雏鸡，测定鸡体ＨＩ抗体效阶及Ｅｔｇ花环形成率的变化，结果表明，ＬＦＡ－３组抗体效价与对照组差异极显著，ＩＬ－２也可显著提高ＩＶ系苗免疫后ＨＩ抗体效价，且两者的作用具有剂量依赖性。     

Limited awareness of animal influenza prevention and control among Dai Lue smallholder farmers in Southwest China

Awareness of animal influenza and its prevention and control is important for ensuring livestock health, production and welfare. In China, a country stereotyped as a major source of emerging zoonotic infectious diseases, research on the public understanding of animal influenza is limited to the Han, the main ethnic group. The present qualitative study in Southwest China investigated awareness of animal influenza among the Dai, an ethnic minority. The participants (15 men and 10 women, ages 18–83) were smallholder farmers of pigs and poultry in rural areas of Jinghong, Xishuangbanna, Yunnan Province. A mixture of interviews and group discussions took place in homes and villages. The participants were asked about their knowledge of avian influenza (H7N9), swine influenza (H1N1), precautions taken to protect against influenza, procedures when animals were sick and perceived risk of animal influenza. The data were analysed following coding and thematic analysis. The findings demonstrated a limited understanding of animal health and welfare among participants. Specifically, they were largely unaware of animal influenza (H7N9, H1N1) including its causes, symptoms, prevention and treatment. The farmers were also uninformed of the risks they faced and unknowingly engaged in behaviours which increased direct or indirect exposure to infected animals, a risk factor for human infection. They also reported poor usage of veterinary services. In order to guarantee the health, welfare and production of their livestock, immediate action is needed to enable Dai smallholder farmers to prevent and respond to animal influenza effectively and timely.