用雌性系配制的甜瓜新品种‘寿研1号’及其指纹图谱

 厚皮甜瓜‘寿研1号’是以回交转育的雌性系‘白9298gy’为母本, 以‘玉9635’自交系 为父本配制的一代杂交种。中熟, 果实发育期33～36 d。果实高圆形, 单瓜质量115～210 kg, 果皮光滑洁白, 果肉细腻, 白色略带浅绿, 糖度16.5%左右。易坐果, 耐低温, 抗病性强, 适于保护地栽培。用8对引物构建了其重复概率为1 /291 600的SSR分子标记的指纹图谱。     

生根粉在八仙花扦插繁殖中的应用研究简

在八仙花（Hydrangea macrophylla）扦插繁殖中,使用不同浓度的用生根粉配制的溶液,并进行不同施用方式及不同施用时间的处理。试验结果表明,合理使用生根粉,能够有效地提高八仙花扦插繁殖的生根率。     

新疆野生樱桃李SSR体系的建立及应用

为建立适宜新疆野生樱桃李的SSR分子标记技术体系,通过对PCR反应程序、反应体系(DNA模板量、引物浓度、Mg2+浓度、dNTP浓度、Taq酶用量)以及退火温度进行了探索,建立了适宜野生樱桃李的SSR-PCR反应体系。结果表明:在25μL反应体系中,Mg2+1.0 mmol/L、引物0.5μmol/L、dNTPs 0.20 mmol/L、TaqDNA聚合酶1.0 U、模板DNA 30 ng、退火温度为60℃。SSR扩增程序:94℃预变性5 min,35个循环(94℃30 s,60℃1 min,72℃1 min),72℃延伸10 min,可筛选出稳定性好、多态性高的SSR引物。     

Relationship between Th1/Th2 balance and CD28/CTLA-4 expression on peripheral blood T cells in patients with systemic lupus erythematosus

AIM: To investigate the pattern of Th1/Th2 balance in systemic lupus erythematosus(SLE) patients and the relationship between CD28/CTLA-4(cytotoxic T-lymphocyte antigen-4) molecule expression and Th1/Th2 balance.METHODS: Eighteen SLE patients met the ARA 1997 updated SLE criteria were selected in the study. According to Bombardier's SLEDAI criteria, all patients were classified into two groups: active group(12 cases) and static group(6 cases). Fourteen normal individuals, matched for age and sex of the patients, served as controls. The peripheral blood mononuclear cells(PBMCs) were isolated by density gradient centrifugation and cultured in RPMI-1640 culture medium. After treated with PMA(5 μg/L) and ionomycin(500 μg/L) for 72 h, the PBMCs were collected, the contents of IFN-γ and IL-10 in the supernatant of cultured PBMCs were detected using enzyme linked immunosorbent assay(ELISA). The expression of CD28 and CTLA-4 molecules on T cells were detected by flow cytometric technique with double staining by FITC or PE labeled monoclonal antibodies. RESULTS: The level of IL-10 was higher in the PBMCs of active and static SLE patients(351.29 ng/L±153.31 ng/L and 319.37 ng/L±153.39 ng/L) than that in controls(254.48 ng/L±120.69 ng/L), but the difference did not reach statistical significance(P>0.05). The level of IFN-γ was significantly lower in the PBMCs of active SLE patients(25.76 ng/L±16.09 ng/L) than that in controls(50.71 ng/L±27.92 ng/L, P＜0.05). The ratio of IL-10/IFN-γ was significantly higher in active SLE patients(18.74±13.77) than that in controls(6.66±4.95, P<0.05). Either before or after culture, the expression of CD28 molecule on CD3⁺and CD8⁺ T cells from all SLE patients was not remarkably different from that in the cells of controls. Before culture, the expression of CTLA-4 molecule on CD3⁺T cells of active SLE patients(0.79%+0.37%) was significantly lower than that in the cells of controls(1.31%+0.61%, P<0.05). After culture, the expression of CTLA-4 molecule on CD3⁺ T cells of SLE patients was still lower than that in the cells of normal controls without statistical significance(P＞0.05).The expression level of CD28 molecule on CD3⁺ or CD8⁺ T cells in active SLE patients and controls was not correlated with the levels of IFN-γ and IL-10 in the supernatants(P＞0.05). The level of CTLA-4 molecule expression on CD3⁺ T cells of active SLE patients was positively correlated with IFN-γ level(r=0.681, P<0.05), while was negatively correlated with IL-10 levels(r=-0.624,P＜0.05) and the ratio of IL-10/IFN-γ(r=-0.738, P<0.01). The level of CTLA-4 molecule expression on CD3⁺ or CD8⁺ T cells of controls showed no correlation with IFN-γ levels, while showed negative correlations with IL-10 level(r=-0.587, P<0.05; r=-0.563, P<0.05, respectively).CONCLUSION: There is a bias in the differentiation of Th0 cells towards Th2 in SLE patients. CTLA-4 probably plays an important role in this mechanism through suppressing the signal transmitted by CD28.     

辣椒雄性不育三系品种江淮8号的选育

江淮8号是以辣椒雄性不育系A-921为母本、甜椒雄性不育恢复系C-097为父本配制的辣椒一代杂交新品种。该品种早中熟,植株高度约65 cm,植株开展度约60 cm,株型紧凑,首花节位10~11节,挂果集中,果实牛角形,青果绿色,果面光滑,果长度12 cm左右,果肩宽度6 cm左右,平均单果质量约65 g,辣味中等。667 m2产量约3 800 kg,抗疫病、炭疽病等病害,耐热耐湿,适应性强。     

三种香料蔬菜的营养及挥发性物质分析

对百里香、罗勒、龙蒿草3种香料蔬菜的营养物质含量及挥发性化学物质成分进行了分析,并与普通蔬菜的文献值进行比较。结果表明:3种香料蔬菜的蛋白质、碳水化合物及无机营养元素钙、铁、锌和硒、维生素C含量比普通蔬菜高,而脂肪、胡萝卜素含量与普通蔬菜相比无明显差异。     

杨梅简易气调保鲜过程中气体成分变化及保鲜效果

以东魁杨梅为试材,研究简易自发气调贮藏(MA)和充氮气调贮藏(NA)条件下,杨梅贮藏小环境中气体成分的变化和对贮藏效果的影响。结果表明:在MA中,乙烯浓度在2d达到高峰;O2前4d浓度急剧下降,后维持在2.48%至1.78%;CO2一直呈上升趋势,试验过程浓度提高了124.3%。在NA中,乙烯高峰出现时间明显推迟、峰高降低;O2浓度维持在2.28%-1.82%,CO2变化与MA相似,浓度提高了131.3%。MA和NA均有效延缓杨梅鲜果的软化变质,减缓果实硬度和总糖的下降速率。贮藏至16d,NA和MA中果实变质率分别比对照下降31.6%和22.5%,硬度比对照增加25.93%和21.98%,总糖含量处理与对照无明显差异。     

东莞市"绿韵"生态园区生物变化调查

东莞市"绿韵"生态园区经过近4年的建设,植物种类从原来的175种上升到263种,各类品种资源丰富,布局合理;鸟类、蛙类、蜘蛛、蛇类等野生动物日渐增多,有害昆虫种类数从原来的41种降到36种,主要害虫的种群密度在下降,有益昆虫种类数从原来的8种上升到14种,大多数有益昆虫的种群密度在上升;作物病害的种类数从原来的55种下降到34种,一些主要作物病害发病率在下降。     

政府投资项目投资决策体系浅析

改善政府投资项目的投资决策体系具有十分重要的现实意义,这也是政府投资项目管理的重要组成部分。文章从政府投资项目全过程管理的角度,分析了我国现行的政府投资项目的审批制度和投资控制的全过程,针对存在的弊端,提出了建立政府项目投资决策体系的建议。     

樱桃番茄新品种钱江红珠的选育

钱江红珠是以X1R为母本、KY-10-1为父本配制而成的杂交1代樱桃番茄新品种.该品种属无限生长型,中熟,生长势强.果实长圆,大红色,大小均匀,单果质量16～18 g,硬度好,耐贮运,春季667 m2平均产量5000 kg左右,抗TMV(Tobacco mosaic virus),中抗CMV(Cucumber mosa...