Impact of serial activation of embryonic pax2 and WT1 genes on transdifferentiation of renal tubular epithelial cells

AIM: To investigate the mechanisms of transdifferentiation of renal tubular epithelial cells by the observation of serial activation of embryonic pax2 and WT1 genes in chronic renal failure model of 5/6 nephrectomized injury. METHODS: Embryo of mice, cultured renal tubule cells and chronic renal failure rat model of 5/6 nephrectomized injury were established. The expressions of paired Box gene (pax2) and Willm’s tumor gene (WT1), as well as α-smooth muscle actin (α-SMA), a phenotype of mesenchymal cells, were detected by RT-PCR and immunohistochemistry techniques. RESULTS: (1) Expressions of pax2 and WT1 mRNA began at 11.5 d and 14 d of embryo and increased gradually, and expression in trace quantity at 2 weeks after birth. Immunohistochemistry analysis revealed that WT1 only expressed in the glomerular podocytes and expressions of pax2 and WT1 in adult renal tubular cells were negative. (2) Deno-expression of pax2 and WT1 in some tubular cells appeared at week 2 and peaked at week 4 in 5/6 nephrectomized rats, both showing a same trend of expression. However, pax2 showed another peak at week 10 afterwards. (3) Re-expressions of pax2 and WT1 in TEC at 0.5 and 24 h after treated with IL-1α (10 μg/L) or AngII (10-9 mol/L) were observed respectively, followed by upregulation of α-SMA expression, and mesenchymal cells characters were shown. The effects were inhibited by Ang II receptor 2 antagonist and WT1 antibody, respectively. CONCLUSION: Adult renal tubule cells acquire re-activation of embryonic pax2 and WT1 genes and phenotypes of mesenchymes when challenge with certain injuries. Deno-expression of pax2 and WT1 genes closely relates with high concentration of bioactive facors, such as AngII and IL-1, which are involved in the mechanisms of renal tubule cells transdifferentiation.     

黄瓜果实曲直性评价方法及相关性分析

以48份黄瓜品种为试材,采用刨分法测量其果实弯曲度,通过黄瓜果实形态指标和相关分析可知弯曲度与果长、果柄长、果柄长/果长、果长/果横径显著正相关,与种腔大小、种腔/果横径极显著负相关,与果横径显著负相关,与果肉厚不相关。依据果形指数和弯曲度对黄瓜进行了分级。筛选出顺直品种8份,次弯曲品种9份,弯曲品种31份。     

银杏叶对辣椒植株发酵液自毒作用的化感调控

采用1/2霍格兰（Hoagland）营养液水培法,研究辣椒植株发酵液的自毒作用及银杏叶发酵液和 再腐解液对其的调控作用。结果表明,辣椒植株发酵液使辣椒受到自毒作用,根系活力下降,相对电导 率增大。银杏叶发酵液加重了辣椒植株发酵液的自毒作用,并且与其浓度成正相关。银杏叶再腐解液缓 解了辣椒的自毒作用,缓解效果与再腐解液的浓度有关。     

西瓜主要农艺性状与裂果性状的相关及通径分析

对西瓜的14个农艺性状进行相关与通径分析。相关分析结果表明,裂果率与果皮厚度、外果皮木质化程度呈显著或极显著相关。经过逐步回归,10个性状与裂果有直接和间接效应,决定系数为1.000,剩余通径系数为0.001 8。外果皮木质化程度对裂果的直接作用最大,直接通径系数达到0.796,可将其作为筛选抗裂品种的重要参考性状。     

食用菊花扦插繁殖影响因素的初步研究

从基质选择、插穗长度、生根粉处理及食用菊花品种几个方面,对影响食用菊花扦插繁殖成活率的有关因素进行了研究。结果表明,珍珠岩是食用菊花扦插的首选基质;插穗长度为10 cm时生根情况较好;生根粉能够显著促进插穗生根;食用菊花插穗生根能力不同品种间差异较大。     

甘蓝小孢子植株倍性简易鉴定方法研究

以编号为12、22的甘蓝材料（F1）经小孢子培养得到的植株为试材,先利用流式细胞仪对供试材料进行倍性鉴定,再利用普通光学显微镜观察已通过倍性鉴定的双单倍体与单倍体的气孔保卫细胞,以测定其大小与叶绿体数。结果表明：气孔保卫细胞叶绿体数与甘蓝倍性高低呈正比关系,甘蓝双单倍体气孔保卫细胞叶绿体数变异幅度为12~17,单倍体气孔保卫细胞叶绿体数的变异幅度为6~10;同一材料双单倍体与单倍体气孔保卫细胞叶绿体数的比值≈2,两者数值差异非常显著直观,易于辨别。因此观测气孔保卫细胞叶绿体数是鉴定甘蓝小孢子植株倍性的简易有效手段。     

高产虫草菌素蛹虫草菌株的紫外线诱变选育

蛹虫草是重要的药食用真菌。通过对蛹虫草原生质体进行紫外诱变处理,筛选到1株优良的菌株CMM-81。经固体发酵,虫草菌素和腺苷含量分别为0．437％和0．036％,是出发菌株的2倍。菌株CMM-81经连续15代传代培养,虫草菌素和腺苷的含量保持稳定。     

富硒纳豆芽孢杆菌的选育试验

利用纳豆芽孢杆菌将无机硒生物转化为有机硒后,进行富硒纳豆的发酵试验.结果表明:纳豆芽孢杆菌能够进行无机硒的生物转化,其转化无机硒的最大浓度为1.0×10-6mol/L.过量的无机硒对纳豆芽孢杆菌具有明显的毒害作用,少量的无机硒可促进纳豆芽孢杆菌的生长.通过正交试验优化出富硒纳豆的最佳发酵条件为1 kg大豆中添加硒1.0×10-6mol,接种量7%,培养温度42℃,培养时间18 h,经检测此条件下发酵的纳豆硒含量为6.58μmol/kg,无机硒的转化率达73.6%.     

遮荫处理对侧柏种子萌发的影响

在不同光照梯度55.44%自然全光照(Natural sonlight,NS)、21.12%NS、3.47%NS 和0.86%NS的人工遮荫条件下,研究了侧柏(Platycladus orientalis)种子萌发的特点.结果表明:4个光照梯度处理的侧柏种子的萌发率分别为68.9%、82.2%、44.4%和42.2%;随着光照强度的减弱,种子萌发进程减慢;萌发指数和幼苗活力指数均在21.12%NS处理最大,分别为1.89和770.8.     

送春与多花兰杂种的非共生萌发与快速繁殖

用地生兰送春和附生兰多花兰进行种间杂交,对杂交种子进行非共生萌发和快速繁殖研究.结果表明:胚龄7个月、0.1 mol/L KOH处理10 min的种子萌发率最高;种子萌发后原球茎的增殖以Z4 (KC+BA 0.4 mg/L+NAA 0.6 mg/L+KT 0.2 mg/L ) 培养基为最好,增殖系数可达到12.8;在原球茎增殖和分化成苗过程中加入活性碳能够有效地防止切口褐化.