口蹄疫疫苗效检模型动物测毒及口蹄疫种毒冻干试验

应用豚鼠作为口蹄疫疫苗效检模型动物检测口蹄疫病毒对模型动物的病原性.用体重400 g左右的豚鼠,将猪O型口蹄疫灭活疫苗效检攻毒毒株ORMF8经后肢蹠部皮内注射途径进行测毒.测毒结果表明,口蹄疫病毒可引起豚鼠出现典型发病,并产生明显病变,ORMF8种毒对豚鼠的毒价可达105.5ID50/0.2 mL.将乳鼠中和试验用种毒OMⅡ按一定比例加入5%蔗糖脱脂牛奶稳定剂进行冷冻真空干燥试验,3次冻干试验结果表明,种毒冻干后病毒含量有一定程度的下降,但下降程度不显著.     

丰城市国营坪荫林场毛竹立地分类与评价

就丰城市国营坪荫林场54块毛竹林分样地的实测资料,通过数量化方法,建立林分平均胸径与各立地定性因子的综合数学模型.根据各因子的贡献大小,确定出影响毛竹生长的主导因子,分析它们对毛竹生长影响的规律,并以此为依据,从现有经营水平出发,本着便于应用的原则,对整个林场竹林立地类型划分与评价进行了初步的探讨.     

表达传染性喉气管炎病毒gB基因重组鸡痘病毒疫苗免疫持续期试验

将200003批疫苗免疫4周龄SPF鸡,免疫后的7、14、21、30、60、90、120、150、180和225 d分别采血,分离血清,采用ELISA方法检测血清抗FPV抗体,结果表明重组疫苗免疫后14 d,免疫鸡血清抗体已经全部阳转,免疫后的21 d血清抗FPV的抗体出现峰值,此后便开始下降,到免疫后的6个月抗体水平已经接近阴性对照的水平.用ILTV WG株和FPV 102株强毒进行的攻毒保护试验与血清检测的结果基本一致,在免疫后的5个月内可以使免疫鸡获得100%(10/10)保护,免疫后的6个月对ILT和FP的免疫保护分别为1/7和2/10,此时需要对鸡群实施二次免疫.其他5批疫苗(200001、200002、200101、200102和200103)免疫SPF鸡后5个月用ILTV WG株和FPV 102株攻击也获得了完全保护.     

城市街道绿地土壤浅析

本文对城市街道绿地土壤的特点和植物的生长关系进行了分析，并就城市街道绿地土壤的合理利用及改良提出了一些建议。     

湟源县“三北”防护林体系工程建设(第一阶段)社会影响评价

对青海省湟源县“三北”防护林建设工程(第一阶段)的社会经济影响从生态、经济、社会三个方面进行了分析和评价,认为该项工程改变了当地生态环境,改善了社会经济状况,促进了社会进步,其影响是积极的、巨大的。作为生态脆弱区的典型案例,为国家大规模的生态环境建设,丰富了知识,提供了借鉴。     

Feasibility Study on Mouse Tail Blood Electrolytes Determination by Dry Chemical

In this experiment,dry chemical method and wet chemical method were used to detect electrolyte levels of Cchl1a3-R528H gene knock-in mice,we compared the two different detection methods and analyzed their correlation.Firstly,we tested the precision of the electrolyte analyzer,and then selected the same mouse,collected the whole blood from tail with dry chemical method to detect electrolyte levels and collected the whole blood from the retro-orbital venous plexus with wet chemical method to detect electrolyte levels,and compared the two different detection methods.The results showed that the CV of Na⁺,Cl^- and K⁺ detected by dry electrolyte analyzer were(2.06±0.18)%,(3.61±0.22)% and (4.40±0.19)%,respectively,all < 4.5%.Compared with the results detected by two different detection methods above-mentioned,the difference was not statistically significant (P> 0.05),and there were linear positive correlations between the two detection methods in Na⁺,Cl^- and K⁺(r of Na⁺ and Cl^- were both > 0.975).The result indicated that the dry electrolyte analyzer had high precision and good stability.The detected electrolytes results by two detection methods were with high correlation and good comparability,subsequent experiments could use dry chemical method to replace wet chemical method to detect electrolytes.