Effect of basil polysaccharide on rat model bearing NuTu-19 cells

AIM：To study the effect of basil polysaccharide on the rat model bearing NuTu-19 cells.METHODS：The rat model bearing NuTu-19 cells was treated with basil polysaccharide.The changes of the tumor cell biology and histopathology were observed.Total RNA was isolated from the tumor tissue.Hybridization assay was performed using gene expression microarray.The results were analyzed by photo disposal software-GenePix Pro3.0.RESULTS：Compared with control,the amounts of ascites in the rats treated with basil polysaccharide were decreased (P<0.05) and the scores of abdominal carcinoma metastasis were changed significantly (P<0.01).268 differentially expressed genes were found in the tumor specimen from the rats exposed to basil polysaccharide.CONCLUSION：Basil polysaccharide may be a new anti-tumor drug and it has multi-targets.Gene expression microarray has huge advantage and value in the study of the drug target.     

Rapid migration of host lymphocytes into the lymphoid tissue of the intestinal graft at early stage after transplantation

AIM:To determine the migration mode of host lymphocytes into the intestinal graft by analyzing different lymphoid compartments of the graft. METHODS:2, 4, 6 days after transplantation, murine intestinal grafts were harvested for histologic assessment. Lymphocytes were collected from mesenteric lymph nodes (MLN), Peyer’s patch (PP), lamina propria lymphocytes (LPL) and intraepithelial lymphocytes (IEL) in the graft for analyzing by cytometry. RESULTS:At the day 2 and day 4, no evidence of rejection in intestinal allograft was found, while the rejection signs were apparent at the 6th day. At day 2 and day 4, host lymphocytes had rapidly replaced donor cell in graft MLN and PP. The migration of host lymphocytes into the LPL and IEL was not as fast as MLN and PP, however, the near-total replacement took place at the 6th post transplant day. The host lymphocytes were activated at the early stage of recruitment into the graft. CONCLUSION:The rapid recruitment of recipient lymphocytes and early activation could be responsible for the formidable rejection of intestinal transplantation.     

Proliferation and apoptosis of neuroendocrine cells in ovarian epithelial tumors

AIM: The purpose of this study was to observe the morphological features of neuroendocrine cells (NECs)， their proliferation and apoptosis in ovarian epithelial tumors, and to discuss their biological and clinical significance. METHODS: 79 specimens of ovarian epithelial tumor samples were collected, of them 20 benign, 18 boderline, 41 milignant tumors, and 22 normal ovaries were investigated immunohistochemically. Chromogranin A was used to detect NECs and their proliferation and apoptosis were examined by double-label staining of chromogranin A and Ki67 or TUNEL. RESULTS: The positive rate of CgA, distribution and staining intensity in ovarian epithelial tumors were higher than those in normal ovary. NECs showed various shapes with neuronoid protuberances stretching to the neighboring cells or basement membrane. Occasionally, they might touch together. No TUNEL positive coexpression in all NECs was observed by double-label staining, but some NECs were coexpressed with Ki67. CONCLUSION: NECs of ovarian epithelial tumors like cancer cells showed a proliferation, but no apoptosis. Their secretion might promote their neighboring non-NECs to proliferate and prevent them from apoptosis.     

富士苹果施钙肥效应

以8年生富士苹果(Malusdomesticacv.Fuji)为试材进行不同组合钙肥试验,结果表明,土施CaSO4肥显著提高了土壤总钙、交换性钙和果实钙含量,以3.5kg/株CaSO4处理效果最显著;将3.5kgCaSO4分为幼果期和膨大期2次使用增钙效果明显,分期施肥无论配合秋施有机肥还是春季喷氨基酸钙都极显著提高了果实钙含量,以S2+1.5AM处理(春施2kgCaSO4+喷3遍氨基酸钙+夏施1.5kgCaSO4+秋施5kg苹果专用有机肥)的钙含量最高,达到179.17mg/kg;秋施苹果专用有机肥明显提高果实钙含量,增加根系和枝条的贮藏钙,以10kg/株配合春施1kg/株CaSO4的效果较好。当有机肥施用量达到15kg/株或CaSO4超过3.5kg/株时增钙效果下降。交换性钙、土壤及果实Ca/Mg比值与果实钙含量呈正相关,土壤速效磷、钾、果实N/Ca比值与果实钙含量呈显著负相关。不同施肥处理果实的N/Ca比值和Ca/Mg比值差异显著,采收时富士果实N/Ca比值<17,Ca/Mg比值≥7,贮藏期间的苦痘病发生率较低。有利于富士苹果钙素营养的施肥组合是秋冬施有机肥加钙化肥、幼果期喷钙和果实膨大期土施钙肥。     

西瓜成熟花粉的超微结构

为了解西瓜成熟花粉的超微结构特点,特别是营养细胞和生殖细胞之间的关系,选取刚开花的西瓜雄花采集花粉,2.5%戊二醛固定并制成超薄切片,透射电镜观察照相。结果表明,西瓜成熟花粉粒中营养细胞含丰富脂类储藏物质和内质网,内质网堆叠,周围和间隙中充满脂类物质;含丰富高尔基体和大量小泡;质体很少。营养核通常深度开裂,产生多数裂片,裂片伸向生殖细胞。生殖细胞流线型,细胞壁电子透明,细胞核伸长,细胞质含高尔基体、内质网,不含质体。生殖细胞表面产生波状突起,波峰背离营养核。生殖细胞一端伸入营养核裂片间隙,2者构成雄性生殖单位。     

大扁杏整形修剪中的问题及解决办法

大扁杏是优良甜仁杏的总称，与山杏（苦杏仁）相比，不但具有山杏的抗旱、耐瘠薄、适应性强、管理省工、产品耐贮、耐运的特点，而且是经济价值特别高的经济林树种，被群众称为“铁杆庄稼”。其仁、肉、核壳均可进行综合利用，国内外有着极大的市场。我地区大扁杏发展很快，但是在大扁杏整形修剪技术上存在着诸多的问题，已经严重影响了产量和效益的提高。[第一段]     

中国樱桃品种‘诸暨短柄樱桃’

 ‘诸暨短柄樱桃’是从地方野樱桃中提纯并筛选出的优质大果中国樱桃品种, 果实大, 外形美观, 品质优, 平均单果质量2.8 g, 最大4 g, 可溶性固形物含量13.8% , 可食率89%。     

牛蛙(Rana catesbeiana)蛙皮抗菌肽基因的克隆、测序及其表达

利用RT—PCR的方法从牛蛙皮肤组织中克隆到大小为270bp的片段RCABP，将其克隆到pGEM—T载体，测序获得1个新的碱基序列。将此基因克隆到原核表达载体pQE一80L，获得融合表达质粒pQE一80L／DHFR／ABP，在1％IPTG诱导下进行表达。SDS—PAGE检测表明，重组蛙皮抗菌肽蛋白的表达量占菌体总蛋白的24％，以包涵体形式存在。体外抑菌试验表明，所构建的质粒能在大肠杆菌中表达具有体外抑菌活性的蛙皮抗菌肽，该融合蛋白具有良好的应用前景。     

山羊乳腺上皮细胞的生长特性

用组织块培养法获得山羊乳腺细胞原代培养物。根据山羊乳腺成纤维细胞与上皮细胞对胰蛋白酶的敏感性不同将二者分离纯化，对细胞生长特性进行了光镜观察。结果如下：细胞可形成闭合型细胞群和开放型细胞群。乳腺上皮细胞与成纤维细胞混生时，细胞之间形成许多腔状结构。纯化的乳腺上皮细胞通过单细胞悬浮后传代，部分细胞形成岛屿状聚集，部分细胞以贴壁的自由单个细胞散在形式存在。上皮细胞增殖可形成圆顶型结构，呈乳头状，称之为乳球体；可产生乳腺上皮细胞并分泌乳汁。山羊乳腺上皮细胞含不同的细胞类型．大多数上皮细胞呈短梭形或多角形。细胞之间紧密相靠。互相衔接。连接成片，呈蜂窝状；细胞核呈圆形或椭圆形．核仁2～4枚；部分细胞呈圆饼状，体积较大；出现部分长形细胞；接触抑制的上皮细胞形态不均一。纯化的山羊乳腺上皮细胞传代至第15代时其生长仍正常，经透射电镜观察发现细胞表面微绒毛极为发达，细胞质中线粒体和粗面内质网丰富，细胞质内有大量脂滴及小泡，表明第15代山羊乳腺上皮细胞增殖活力旺盛。染色体数目分析表明．该细胞系稳定，在离体培养条件下细胞不发生转化。山羊乳腺上皮细胞系的细胞染色体数目为60．染色体组型为2n=60。