应用热脉冲技术对小美旱杨耗水量的研究

应用热脉冲对河套灌区农田防护小美旱杨（Populus popular’s）日蒸腾耗水量进行了研究。结果表明：在正常生长状态下，小美旱杨的日蒸腾耗水量变化曲线呈宽峰状，随着林龄增加，日蒸腾耗水量逐渐增加，在不同水分条件下,灌水时日蒸腾耗水量：萌生苗日蒸腾耗水量大于扦插日蒸腾耗水量。     

The effect of exploratory laparotomy on the serum and peritoneal haptoglobin concentrations of the pony.

Serum haptoglobin concentration was used as an indicator of the acute phase response in ponies undergoing exploratory laparotomy. Preoperative, 1 h intraoperative, 3 h, 6 h, 12 h and 24 h postoperative blood samples and 48 h postoperative peritoneal fluid samples were obtained for haptoglobin analysis. A spectrophotometric assay based on cyanmethemoglobin binding capacity (CyanBC) was used to determine haptoglobin concentrations. The preoperative reference range for serum haptoglobin concentrations in these ponies was 25-60 mg CyanBC/dL. Intraoperative and 3 h postoperative blood samples had decreased haptoglobin concentrations when compared to preoperative values. Serum haptoglobin concentrations began to rise by the 6 h postoperative sample and were generally elevated above preoperative values by the 24 h postoperative sample. Two of the ten ponies had mild signs of postoperative colic which were associated with twofold elevations in serum haptoglobin concentrations and fivefold elevations in peritoneal fluid haptoglobin concentrations.     

Quantitation of adenine nucleotides in equine colonic mucosal tissue using high performance liquid chromatography.

The objectives were to use high performance liquid chromatography (HPLC) to validate an established method for adenine nucleotide separation in equine colonic mucosal tissue, to determine the inherent variability in the tissue and extraction method, and to determine the stability of ATP, ADP, and AMP in the tissue with time. Equine colonic mucosal tissue obtained from a single horse was immediately submersed in liquid nitrogen, and stored at -70 degrees C. Samples were lyophilized, extracted, and separated by HPLC. The limit of quantitation was 0.05 microg/mL. The coefficient of variation for the instrument was less than 10% for all nucleotides measured. When the tissue was not homogenized prior to sampling, there were significant differences in adenine nucleotide content between samples. However, when the tissue was homogenized prior to analysis, these differences were no longer significant. There was no significant decrease in ATP, ADP, or AMP content over a 54-day analysis period.     

Transmission of porcine reproductive and respiratory syndrome virus from persistently infected sows to contact controls.

The objective of this study was to determine if porcine reproductive and respiratory syndrome virus (PRRSV) could persist in non-pregnant sows and if persistently infected sows could transmit virus to naive contact controls. Twelve PRRSV-naive, non-pregnant sows (index sows) were infected with a field isolate of PRRSV and housed in individual isolation rooms for 42 to 56 days postinfection. Following this period, 1 naive contact sow was placed in each room divided by a gate allowing nose-to-nose contact with a single index sow. Index sows were not viremic at the time of contact sow entry. Virus nucleic acid was detected by polymerase chain reaction, and infectious virus was detected by virus isolation in sera from 3 of the 12 contact sows at 49, 56, and 86 days postinfection. All 3 infected contacts developed PRRSV antibodies. Virus nucleic acid was detected in tissues of all of the 12 index sows at 72 or 86 days postinfection. Nucleic acid sequencing indicated that representative samples from index and infected contacts were homologous (> 99%) to the PRRSV used to infect index sows at the onset of the study. This study demonstrates that PRRSV can persist in sows and that persistently infected sows can transmit virus to naive contact animals.     

尾巨桉试管苗移栽基质配方的研究

以五色土、堆沤木糠渣、锯木屑、泥炭为供试基质材料，进行尾巨桉3229试管苗移栽基质配方筛选研究。结果表明，五色土和锯木屑(1：2、1：1、2：1)三个配比的混合基质均可作为尾巨桉3229试管苗的移栽基质，其成活率高(达98．21％以上)和生长快，与对照(五色土)的成活率96．43％相近；纯五色土和泥炭(1：2和1：1)二个配比的混合基质也可作为尾巨桉试管苗的移栽基质。     

节瓜常温贮藏品质的变化

节瓜高产抗病,肉质清甜,嫩瓜和老熟瓜均可食用,老熟瓜耐贮性好,室温下即可贮藏2~3个月,与目前国内蔬菜贮藏保鲜常用的低温贮藏法相比,成本低、操作方便、保鲜期长。本试验研究了节瓜在常温贮藏过程中水分、可溶性糖、VC含量及风味品质的变化,以期找出节瓜常温贮藏的最佳贮藏期限。1材料与方法以广西主栽节瓜品种桂优1号(广西农业科学院蔬菜研究中心提供,粉皮类型)为试材,2003年2月20日定植,7月4日采收,取花后50d(天)无虫害、无机械损伤的老熟瓜40个,自然温度下置于通风室内架贮。在贮架上铺1层纸板,把瓜平放在架上,堆放2层,贮藏期间不进行…     

免征农业税对农用土地的影响

为了明确免征农业税与农用地利用的关系,采用文献资料法和归纳分析法研究免征农业税对农用土地的影响———改变土地税收结构和农用土地收益分配结构。免征农业税将打开农用土地利用的新局面,对于农用土地使用权流转、资源优化配置、农用土地经营规模、保护耕地等都将产生积极的作用。     

邓恩桉组培中影响继代增殖条件的研究

探讨抗寒树种邓恩桉组培快繁中影响其继代培养的因素。实验表明,继代培养以改良MS为基本培养基,随继代次数的增加逐渐减少细胞分裂素BA和生长素NAA的浓度。最初几次继代,BA的浓度为0.5 mg/L,NAA的浓度为0.2 mg/L。然后BA的浓度逐渐降低到0.1~0.3 mg/L,NAA的浓度逐渐降至0.05~0.1 mg/L,继代5~6次后,BA的浓度保持在0.1~0.3 mg/L,NAA的浓度保持在0.05~0.1 mg/L。继代周期随季节而不同,夏季为15~25 d,春秋季为20~35 d,冬季为40~50 d,芽的月增殖系数为3~5倍,满足工厂化育苗的要求。组培苗的玻璃化和遗传变异等异常现象很少,节间长度适中,有利于生根。     

西南交通大学郫县新校区绿化环境设计中交流氛围的营造

现代化教育的发展使得高等学校的功能不断地扩展，对内对外交流与合作的功能正在日益显现。这要求在高校校园绿化环境设计中体现和满足这一功能。提出了在绿化环境设计中营造交流氛围的原则，并在西南交通大学郫县新校区绿化环境设计中对交流氛围的营造作了初步探索和实践。