改良型强化栽培条件下两优培九生育后期群体特性研究

对杂交水稻组两优培九在不同改良强化栽培条件下的群体形成特性进行了比较分析。结果显示，在改良强化栽培条件下，水稻生育后期的功能叶面积下降缓慢，叶绿素衰减幅度减小，粒叶比增大，群体质量明显改善，显著提高水稻产量。采用SRI核心技术，双本插植与单本插植产量差异不显著；直播可以减少水稻的插植的用工量，还可以获得较高的经济产量。     

柑橘新品种‘金葵蜜橘’

 ‘金葵蜜橘’是由广东省佛冈县‘沙糖橘’园中的变异优株选育而成。生长健壮,果实紧实,扁球形,单果质量39.7 g,果皮橙红色,果肉脆嫩,可溶性固形物含量15.50% ~16.19%,总酸0.46% ~ 0.89%,总糖11.60% ~ 14.87%,维生素C 0.379 ~ 0.465 mg · mL-1,无核,品质优良,成熟期比普通‘沙糖橘’早15 d左右。适宜在广东省‘砂糖橘’适栽区种植。     

Role of translationally controlled tumor protein in PRL-3-promoted metastasis of colon cancer cells

AIM: To study the role of translationally controlled tumor protein (TCTP) in the proliferation, migration and invasion of phosphatase of regenerating liver-3(PRL-3)-promoted colon cancer cells.METHODS: The vectors pAcGFP-C3 and pAcGFP-C3-PRL-3 were constructed and transfected into the colon cancer cell line LoVo.LoVo-PRL-3 cells stably expressing PRL-3 and LoVo-control cells were established. The expression levels of PRL-3 and TCTP in both cells were detected by Western blotting and real-time PCR. The specific siRNA sequence for TCTP mRNA and control-siRNA were synthesized and transfected into the LoVo-PRL-3 cells. TCTP expression at mRNA and protein levels in LoVo-PRL-3 was detected by Western blotting and real-time PCR 24 h, 48 h and 72 h after transfection. The proliferation, migration and invasion abilities of LoVo-control cells, LoVo-PRL-3 cells, TCTP-siRNA and control-siRNA cells were detected by CCK-8 assay and the method of Transwell cell culture chambers.RESULTS: The expression of TCTP at mRNA and protein levels in LoVo cells was significantly increased after PRL-3 transfection (P<0.05). TCTP mRNA was significantly inhibited 24 h, 48 h and 72 h after transfection of TCTP-siRNA (P<0.01). TCTP protein was also significantly inhibited 48 h and 72 h after transfection (P<0.01). Compared with LoVo-control cells, the proliferation, migration and invasion abilities of LoVo-PRL-3 cells were significantly enhanced (P<0.05). However, lowering the up-regulated expression of TCTP in LoVo-PRL-3 cells inhibited the proliferation, migration and invasion abilities (P<0.05). CONCLUSION: PRL-3 promotes proliferation, migration and invasion of colon cancer cells by up-regulating the TCTP expression. siRNA targeting TCTP may be an effective method for prevention and treatment of colon cancer cell metastasis.     

花椰菜—黑芥体细胞杂交获得抗黑腐病异附加系新材料

 以花椰菜—黑芥体细胞杂种的自交及回交后代为材料,建立了结合抗病性人工接种鉴定,流式细胞仪DNA含量分析,B基因组特异分子标记和染色体原位杂交的大规模群体分析技术,在50份高代自交及回交材料中筛选获得了20份形态学特征偏向花椰菜,30份呈花椰菜—黑芥中间类型的材料。细胞DNA含量检测（FLC）及染色体计数结果表明,偏花椰菜的类型,DNA含量基本在2.24 pg · cell^-1以下,染色体数在28条以下。经连续3年黑腐病病菌人工接种鉴定,获得高抗株系12个,抗性株系17个,占全部受试材料的14%。对其中3个（PFCN29 BC3-3-5、PFCN16-1 S2BC3-107和PFCN14-1 S1BC4-123）形态偏花椰菜且高抗黑腐病的植株进行基因组原位杂交,结果显示其分别为花椰菜染色体组附加了2、7和8条黑芥染色体的异附加系材料。这些植株可以用于后续的研究和作为育种桥梁材料,进一步获得黑腐病抗性的单体附加系或渐渗系。本研究表明通过体细胞杂交转育野生优异抗性资源,创新甘蓝类蔬菜育种种质的可行性。     

Establishment and evaluation of rat heart ischemia-reperfusion injury model in vivo

AIM: To establish and evaluate a rat model of heart ischemia-reperfusion injury in vivo. METHODS: Seventy-two male Sprague-Dawley rats weighing(250±50)g were randomly divided into sham operation group(sham), ischemia-reperfusion group(I/R) and normal group. The animals were anesthetized and heparinized. Myocardial ischemia-reperfusion was induced by ligating the left anterior descending coronary artery with "U-shape tube" for 35 min followed by 120 min or 240 min reperfusion in vivo. The heart infarct size was measured by triphenyltetrazolium chloride(TTC) staining. The myocardial cell apoptotic index was determined by the method of terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling(TUNEL). Immunohistochemical method was used to detect the expression of Bcl-2 and Bax in rat ischemia myocardium. The blood level of MB isoenzyme of creatine kinase(CK-MB),cardiac troponin I(cTnI),nitric oxide(NO),malondialdehyde(MDA), total superoxide dismutase(T-SOD)and glutathione peroxidase(GSH-Px) were detected after reperfusion for 2 h and 4 h. RESULTS: Compared with normal group and sham group, there were obvious changes of ST-T segment and Q wave in the electrocardiogram of I/R group. The blood level of CK-MB, cTnI, NO, MDA and GSH-Px in I/R group increased(P<0.05,P<0.01) after reperfusion for 2 h and 4 h, and the blood level of T-SOD in I/R group after reperfusion for 2 h and 4 h also increased(P<0.05). The heart infarct size in I/R group was the largest as compared to other groups. Among these groups, the apoptotic index of I/R group was the highest and the Bcl-2/Bax ratio in I/R group decreased(P<0.01).CONCLUSION: The rat model of heart ischemia-reperfusion injury in vivo can be successfully established with the "U-shape tube". There are obviously changes of heart infarct size, blood level of CK-MB, cTnI, NO, MDA, T-SOD and GSH-Px, myocardial apoptotic index and Bcl-2/Bax ratio between I/R rats and control animals.     

Lenalidomide alone or combined with bortezomib inhibits growth and induces apoptosis in KM3 cells

AIM: To investigate the effects of lenalidomide alone or combined with bortezomib on induction of apoptosis in multiple myeloma(MM) KM3 cells.METHODS: The KM3 cells were treated with lenalidomide alone or combined with bortezomib. The number of viable cells was determined by trypan blue exclusion. Apoptotic cells were simultaneously stained with annexin V-FITC and PI and determined by flow cytometry. RT-PCR was used to examine the level of p65 mRNA. Western blotting was used to examine the protein levels of P65, pP65, caspase-3,-8, -9, and poly (ADP-ribose) polymerase (PARP). RESULTS: Lenalidomide-inhibited the cell growth and induced apoptosis of KM3 cells. The mechanism responsible for lenalidomide-mediated inhibition was via NF-κB and activation of caspase-mediated induction of apoptosis. A synergistic effect was observed when combination of lenalidomide with bortezomib was used. CONCLUSION: Lenalidomide inhibits the growth and induces apoptosis in KM3 cells. There is a synergistical effect when combination of lenalidomide with bortezomib is in use.     

早熟萝卜新品种榕研1号的选育

早熟萝卜新品种榕研1号是由雄性不育系6-4-1A与自交系16-1-5配制而成的一代杂种。早熟,耐热,从播种到采收55 d（天）。株高35 cm,开展度40 cm,板叶,肉质根长圆柱形,根长26～28 cm,1/2入土,直径5～6 cm,皮肉均为白色,水分多,品质好。单根质量0.4～0.5 kg,每667 m2产量3 500 kg左右,适合福建省夏、秋季栽培。     

温敏型核雄性不育系番茄T4育性的研究

以温敏型核雄性不育系番茄T4及对照First为试材,通过调查花粉发芽率、自交结实率、果实种子数以及采用Tricine-SDS-PAGE电泳方法分析叶片及花药蛋白质的变化,研究T4的育性转化,并分析其在可育和不育条件下花粉发芽率、自交结实率、果实种子数、叶片及花药蛋白质的差异。结果表明：28 ℃/18 ℃（昼/夜）处理的T4花粉发芽率、自交结实率最高,果实种子数最多,与对照First差异不显著,表明此时T4恢复部分育性|28 ℃/24 ℃和28 ℃/12 ℃处理的T4花粉发芽率、自交结实率和果实种子数与对照差异均达到极显著水平,表现为不育。不同温度处理的T4花药蛋白质与叶片蛋白质差异均达到显著水平|28 ℃/24 ℃和28 ℃/12 ℃处理的T4叶片蛋白质与对照差异显著,但28 ℃/18 ℃处理的差异不明显。     

复式断面明渠分析解应用研究

复式断面明渠中,二次流对水流特性具有重要的影响.现有复式断面横向速度分布的分析解模型中,对二次流的表示有多种形式.针对其中两种常用的考虑二次流影响的分析解模型在梯形明渠中的应用,采用FCF(Flood Channel Facility)的实验数据对模型进行了检验,结果表明两种模型各有利弊,具体应用中需根据实际情况进行选...     

小样本情况下需水量预测模型研究

针对传统的GM（1,1）模型,分析了其预测结果与实际过程存在的偏差,通过GM（1,1）模型与自回归滑动平均模型相结合的方法以弥补偏差。以钦州市1999-2009年及2005-2009年城市供水总量这两组基础数据对本文所建模型进行验证,并利用此模型预测了钦州市2010-2018年需水量。通过与其他预测模型的对比,进一步证...