桃(Prunus persica L.)茎尖培养技术的研究

筛选出了适合于桃品种‘北农早艳’茎尖(0.2 mm 和0.5 mm)培养的培养基为:G培养基附加 IBA 0.25 mg/L+BA 0.5 mg/L+GA_3 1.0 mg/L+LH 500.0 mg/L。茎尖用聚氨酯海绵作支撑物的液体培养比琼脂培养成活率高2～5倍,且生长迅速。获得了0.2 mm 长茎尖的生根植株。     

Magnesium influence on plant uptake of phosphorus in a calcareous soil

A pot experiment was conducted to investigate the role of magnesium (Mg) in plant utilization of ³²phoshorus (P)‐labelled P in a calcareous soil (Typic Hapluquent). Results with two successive harvests of ryegrass shoots indicated that Mg had no obvious influence on plant uptake of the P applied. In a separate incubation study with the same soil, labelled P along with different levels of Mg was introduced to the soil at two time intervals and soil inorganic P was fractionated. The isotope data of P in various fractions indicated that Mg at the test levels had no obvious influence on P transformation either. It is suggested from the experiments that Mg is unlikely to be able to promote plant utilization of fertilizer P in calcareous soils.     

追踪实战基本技能的训练

警犬追踪实战情况错综复杂,既有客观条件的变化,如案件性质、使用条件、现场环境和地理气候等;又有主观因素的影响,如人的分析判断能力、犬的作业状况等。面对各种各样复杂的现场,带犬民警作为警犬使用的主体,必须具备对案件现场勘察、分析、判断的基本功,具有正确指挥和使用警     

Molecular cloning and expression of kidney‐type glutaminase from common carp (Cyprinus carpio) and its up‐regulation by glutamine in primary culture enterocyte

Glutaminase (GLS) is the key enzyme of glutamine (Gln) metabolism and utilization. In this study, a cDNA encoding GLS protein was identified from common carp Cyprinus carpio intestine. The open reading frame of GLS cDNA encodes a polypeptide of 595 amino acids, which shows a high similarity with its zebrafish Danio rerio counterpart. Bioinformatic analysis showed the protein belongs to kidney‐type GLS. The putative protein has glutaminase domain and ankyrin repeats domain, which are highly conserved among vertebrate orthologues. Real‐time quantitative PCR analysis revealed that the abundance of GLS mRNA was the highest in the white muscle, followed by the brain, eyeball and pituitary. Glutaminase was ubiquitously expressed in all intestinal segments of common carp. The activity of GLS did not distribute uniformly along the entire length of the intestine. In primary culture enterocyte, and the expression of GLS mRNA is up‐regulated quickly and effectively by Gln.     

开阳县田坝渔业小区实施大鲵养殖的技术措施

为摸索名优水产品养殖经验,加快我县水产养殖结构调整,促进我县渔业健康发展,2007年,我县申报实施了《贵阳市社会主义新农村基础设施建设——养殖基地基础设施建设——渔业小区建设》项目,在花梨乡翁昭村田坝组模拟大鲵生态生活习性新建了100口养殖池的大鲵小区,修建了养殖房、管理房、配套饵料鱼池、饵料鱼暂养池、引水管道等基础设施和配套设施,并于2008年3月25日起引种入场养殖。现将1年来小区开展大鲵人工养殖的技术措施报告如下。     

现浇楼板混凝土“二次法”施工工法

阐述了现浇楼板混凝土施工时,混凝土终凝前通过二次振捣的施工方法,愈合混凝土内部和表面初裂缝,以提高混凝土密实度,达到控制混凝土裂缝的目的。     

Growth,dry matter production,phenotypic plasticity,and nutritive value of three natural populations of Dactylis glomerata L. under various shading treatments

Dactylis glomerata L. is a widespread perennial grass species, which has been reported to be adapted to shaded conditions. Its populations thrive in a variety of environments. However, little information is available concerning the comparative response of its natural populations from contrasting environments under the reduced light intensity conditions that exist in silvopastoral systems. The objective of the present study was to estimate the comparative ability of three populations of D. glomerata from northern, central and southern Greece to grow under full sun, 60 % shade and 90 % shade in terms of their growth characteristics, phenotypic plasticity, dry matter production and nutritive value. Shade reduced tillering and dry matter production, increased tiller height and modified leaf characteristics. Under shade fewer leaves were grown simultaneously on the same tiller, but these were longer and thinner compared to full sun. Differentiation in response to shade among the populations examined of D. glomerata was observed mainly for leaf characteristics. The population from Pertouli (central Greece) responded better, particularly to moderate shade as it had a higher leaf area, longer leaf and higher dry matter production, compared to the others. Evidence for adaptive phenotypic plasticity to moderate shade was suggested only for this population. Additionally, Pertouli had higher nutritive value compared to Taxiarchis (northern Greece) and Crete (southern Greece) under shade. The divergent responses of natural populations of D. glomerata could justify breeding germplasm with enhanced shade tolerance.     

Woher kommen wir und wohin gehen wir? – Analyse des deutschen Erwerbsobstbaus anhand eines Branchenlebenszyklus-Modells

Cassia et al. (2006) claim that each industry develops along a specific industry life cycle with four stages: introduction, growth, maturity and decline. This essay tries to analyze the German fruit-growing industry by the means of the industry life cycle model. The main aspects of the analysis are “consumption and prices”, “competition”, “product innovation” and “process innovation”. In summary, the German professional fruit-growing industry might be classified as to be in the mature stage.     

非洲菊切花保鲜剂的研究

本文研究由蔗糖、硝酸银、8-HQ(8-羟基喹啉)配制的不同保鲜剂对非洲菊切花插瓶寿命、水分平衡、鲜重变化及观赏值等指标的影响。结果表明：4％蔗糖 200mg／L硝酸银 200mg／L硫酸铝 320mg／L8-HQ的保鲜效果最好。     

Effects of contact dermatitis on hepatic gene expression in broilers

1.?Severe foot and hock dermatitis in broiler chickens can reduce growth rate and increase susceptibility to bacterial infection, affecting both profitability and welfare. However, little is known about the underlying physiological changes associated with foot and hock lesions.

2.?This study compared global hepatic gene expression in control birds and those with ammonia-induced foot and hock lesions using Agilent 44?K chicken oligonucleotide microarrays (8 birds per group).

3.?In total, 417 genes were differentially expressed of which 174 could be mapped onto the genome. Genes associated with energy metabolism, thyroid hormone activity and cellular control were affected, while there was also evidence of an up-regulation of genes linked to a pro-inflammatory response.

4.?It is conceivable that pain is the underlying cause for the observed changes in energy metabolism genes.

5.?Changes in hepatic gene expression provide new information on how a chicken's physiological mechanisms alter to cope with foot and hock lesions. The findings support other data indicating that birds with increased severity of lesions are likely to be in pain and that growth will be compromised. Reduction of the incidence of dermatitis by improved husbandry should therefore benefit both welfare and commercial performance.