全文获取类型
收费全文 | 353篇 |
免费 | 9篇 |
专业分类
林业 | 15篇 |
农学 | 15篇 |
42篇 | |
综合类 | 16篇 |
农作物 | 14篇 |
水产渔业 | 41篇 |
畜牧兽医 | 162篇 |
园艺 | 5篇 |
植物保护 | 52篇 |
出版年
2022年 | 4篇 |
2021年 | 6篇 |
2020年 | 2篇 |
2019年 | 5篇 |
2018年 | 3篇 |
2017年 | 8篇 |
2016年 | 9篇 |
2015年 | 9篇 |
2014年 | 13篇 |
2013年 | 18篇 |
2012年 | 22篇 |
2011年 | 29篇 |
2010年 | 9篇 |
2009年 | 14篇 |
2008年 | 22篇 |
2007年 | 35篇 |
2006年 | 11篇 |
2005年 | 26篇 |
2004年 | 31篇 |
2003年 | 20篇 |
2002年 | 15篇 |
2001年 | 10篇 |
2000年 | 7篇 |
1999年 | 4篇 |
1998年 | 5篇 |
1997年 | 4篇 |
1996年 | 2篇 |
1995年 | 3篇 |
1990年 | 3篇 |
1989年 | 3篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 2篇 |
1985年 | 1篇 |
1982年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1975年 | 1篇 |
1972年 | 1篇 |
排序方式: 共有362条查询结果,搜索用时 31 毫秒
21.
H Nagano K Hayashi M Kubo Y Miura 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(2):307-314
In October, 1988, bovine ephemeral fever (BEF) occurred in Nagasaki Prefecture and throughout Kyushu island, with the exception Miyazaki Prefecture. The first outbreak occurred in Hirado-shi on October 17. The total number of diseased cattle was 24 in 24 farms in Nagasaki Prefecture. The clinical findings were mainly sudden fever, anorexia, and instability in standing. The serum neutralizing antibodies against BEF virus (BEFV) rose in all infected cattle. Twelve strains of the virus were isolated in HmLu-1 cell cultures made directly from the heparinized blood of 17 infected cattle. The buffy coat was mainly collected from the samples and washed three times with phosphate buffered saline. These isolates were all neutralized by an antiserum against BEFV (Yamaguchi strain). With the aid of an electron microscope, a representative of isolates named Hirado-9 with a length of 150 nm was seen in the sample of infected HmLu-1 cell cultures. Both Hirado-9 and Yamaguchi strains reacted with antisera. The outbreak of BEF in 1988 was the first since 1971 in Nagasaki Prefecture. The result proved that BEFV can be easily isolated in HmLu-1 cell culture from the washed blood cells of infected cattle. 相似文献
22.
Taharaguchi S Nagano A Okai K Miyasho T Kuwano M Taniyama H Yokota H 《The Veterinary record》2007,161(10):338-342
The objective of this study was to find serum indicators of gastric ulcers in foals. By using two-dimensional electrophoresis of serum proteins, three distinct spots were detected in samples from foals with gastric ulcers detected endoscopically. One of them appeared with high frequency and was identified by partial digestion with trypsin and subsequent nano-electrospray ionisation-tandem mass spectrometry (nanoesi-ms/ms) analysis as an alpha(1)-antitrypsin. Western blot analysis, using an antibody against human alpha(1)-antitrypsin, revealed at least two bands, of molecular weight 58 kDa and 55 kDa, in the sera. The 55 kDa band was detected in 44 of 47 serum samples from foals with gastric ulcers, but in only three of 22 serum samples from healthy foals. 相似文献
23.
Influence of Acid Deposition on Inland Water Chemistry - A Case Study from Hyogo Prefecture, Japan -
The Influence of acid deposition on stream and lake water chemistry was studied in a forested watershed of Hyogo prefecture, Japan. Monthly sampling of four streams, one artificial lake, and precipitation was carried out from 1995 to 2000. The pH of the monthly bulk precipitation and rainwater were ranged from 4.06 to 7.10. No trends were evident during the monitoring periods. The pH and alkalinity in the four streams, which flow into the artificial lake, ranged from 6.37 to 8.72 and 0.077 meqL?1 to 0.485 meqL?1, respectively. The differences in the water quality of the four streams were related to the geology of each watershed. Lower pH and alkalinity were observed during high- discharge periods. On the other hand, the pH and alkalinity of the outflow from the lake ranged from 6.47 to 7.36 and 0.195 meqL?1 to 0.339 meqL?1, respectively. No acidification of the aquatic environment was observed during the investigated periods. The results suggest that this forested ecosystem has the capacity to neutralize incoming acid deposition. 相似文献
24.
The Air noncoding RNA epigenetically silences transcription by targeting G9a to chromatin 总被引:1,自引:0,他引:1
Nagano T Mitchell JA Sanz LA Pauler FM Ferguson-Smith AC Feil R Fraser P 《Science (New York, N.Y.)》2008,322(5908):1717-1720
25.
We found that, in the mouse visual cortex, action potentials generated in a single layer-2/3 pyramidal (excitatory) neuron can reliably evoke large, constant-latency inhibitory postsynaptic currents in other nearby pyramidal cells. This effect is mediated by axo-axonic ionotropic glutamate receptor-mediated excitation of the nerve terminals of inhibitory interneurons, which connect to the target pyramidal cells. Therefore, individual cortical excitatory neurons can generate inhibition independently from the somatic firing of inhibitory interneurons. 相似文献
26.
Food-drug interaction between ferulic acid and nateglinide involving the fluorescein/H+ cotransport system 总被引:2,自引:0,他引:2
Itagaki S Kobayashi Y Otsuka Y Kubo S Kobayashi M Hirano T Iseki K 《Journal of agricultural and food chemistry》2005,53(7):2499-2502
In clinical, patients usually take many kinds of drugs at the same time. Thus, drug-drug interactions involving transporters can often directly affect the therapeutic safety and efficacy of many drugs. However, there have been few studies on food-drug interactions involving transporters. Dietary polyphenols have been widely assumed to be beneficial to human health. Polyphenols are commercially prepared and used as functional foods. We report here for the first time that ferulic acid, which is widely used as a functional food, affects the transport of clinical agents. It is important to be aware of the potential of food-drug interactions and to act in order to prevent undesirable and harmful clinical consequences. 相似文献
27.
28.
Matsuzuka T Ozawa M Nakamura A Ushitani A Hirabayashi M Kanai Y 《The Journal of reproduction and development》2005,51(2):281-287
This study examined the association between redox status in the oviduct and early embryonic death in heat-stressed mice. In Experiment 1, non-pregnant mice were heat-stressed at 35 C with 60% relative humidity for 12, 24, or 36 h, and the maternal redox status was verified by measuring the levels of reactive oxygen species (ROS) and free radical scavenging activity (FRSA) in the oviduct, and thiobarbituric acid reactive substances (TBARS) and glutathione peroxidase (GSH-Px) activity in the liver. In Experiment 2, zygotes were collected from mice heat-stressed for 12 h on the day of pregnancy, and their developmental abilities were assessed in vitro, along with the intensity of DNA damage at the 2-cell stage. The TBARS value and GSH-Px activity in the liver, and ROS level in the oviduct were significantly higher in heat-stressed mice, and this increase appeared to depend on the duration of the heat stress. Maternal heat stress significantly reduced the percentage of zygotes that developed to the morula and blastocyst and the total cell number in the blastocyst. In addition, DNA damage at the 2-cell stage was significantly higher in maternally heat-stressed embryos. These results suggest that heat stress induces systemic changes in redox status in the maternal body, and the resultant increase in oxidative stress in the oviduct is possibly involved in heat stress-induced early embryonic death . 相似文献
29.
Aiso S Takeuchi T Arito H Nagano K Yamamoto S Matsushima T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(10):1019-1029
Carcinogenicity and chronic toxicity of para-dichlorobenzene (p-DCB) were examined by exposing 50 BDF1 mice and 50 F344 rats of both sexes by inhalation to p-DCB vapor at a target concentration of 0 (control), 20, 75 or 300 ppm for 6 hr/day, 5 days/week and 2 years. Incidences of hepatocellular carcinomas, hepatoblastomas and hepatic histiocytic sarcomas in the 300 ppm-exposed male mice, and hepatocellular adenomas and carcinomas and hepatoblastomas in the 300 ppm-exposed female mice were increased. An increase in the incidences of most of those liver tumors was dose-related. No increase in tumor incidence was found in any p-DCB-exposed rat of either sex. Centrilobular hypertrophy of hepatocytes and papillary mineralization and pelvic urothelial hyperplasia of the kidney were noted in the 300 ppm-exposed male rats. Treatment- and age-related increases in incidences of the eosinophilic globules of the respiratory and olfactory epithelia in female rats and incidences of the respiratory metaplasia of the nasal gland epithelium in mice and rats and the olfactory epithelium in mice were noted. The nasal lesion was the most sensitive endpoint of chronic inhalation toxicity. Induction of the mouse hepatocarcinogenicity and lack of the rat nephrocarcinogenicity found in the present study were compared with the mouse liver tumors and the rat renal tumors reported by the NTP gavage study, and discussed in light of the estimated p-DCB uptake into the body through the inhalation and the oral administration. 相似文献
30.
Adam AA Takahashi Y Katagiri S Nagano M 《The Japanese journal of veterinary research》2004,52(2):77-84
Effects of oxygen (O2) tension in the gas atmosphere during in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro culture (IVC) on the efficiency of in vitro production of mouse embryos were examined. Mouse oocytes recovered from large antral follicles were subjected to IVM in Waymouth medium for 15, 16 and 17 hr under 5 or 20% O2 and then subjected to IVF and IVC under 5 or 20% O2 tension. Lowering the O2 tension in the gas atmosphere for IVM from 20 to 5% improved the cleavage rate after IVF when the oocytes were subjected to IVM for 15 hr; however, no improvement in the cleavage rate was observed when the culture period for IVM was extended to 16 and 17 hr. Lowering the O2 tension to 5% for IVM and IVC improved the development of the cleaved oocytes to the blastocyst stage, regardless of the culture period for IVM. However, the O2 tension for IVF had no remarkable effect on the subsequent embryonic development. These results demonstrate that 5% O2 is superior to 20% O2 for IVM and IVC, and suggest that 20% O2 for IVM may delay oocyte maturation and/or the acquisition of fertilizability and impair the developmental competence of oocytes. 相似文献