不同温度对大豆种子萌发影响的研究

通过对东农42、东农43、东农163、合丰25、绥农10号采用六种发芽温度4℃、8℃、12℃、15℃、20℃、25℃下温度对大豆种子萌发影响的研究,表明：4℃、8℃温度过低,在调查期间种子一直没有发芽。其它几个温度的发芽率以15℃为最高,12℃为最低,同前人研究发芽率随温度升高而升高不同。同一温度不同品种的发芽率、平均芽长在品种间差异显著,说明种子萌发受遗传因素影响,同一品种不同温度之间的平均芽长随温度的升高依次升高。实验的研究有利于确定大豆的田间播种温度,为适时早播,保证田间种子的发芽,出苗,成苗,及时的成熟提供依据,并为最终的高产奠定基础。     

Discrimination of ‘San Marzano’ accessions: A comparison of minisatellite, CAPS and SSR markers in relation to morphological traits

‘San Marzano’ (SM) is one of the most widely known tomato (Solanum lycopersicum L.) cultivars, and is a classic example of a local variety with a premium value. Unfortunately, the original cultivated form is underrepresented in the Protected Denomination of Origin (PDO) area because of the incidence of contaminant and phenotypically similar genotypes. Our aim was to examine the ability of three DNA marker systems (minisatellite, cleaved amplified polymorphic sequence (CAPS) and simple sequence repeat (SSR)) to reveal the genetic diversity of tomato accessions that were, based on a morphological analysis, very similar. The data indicate that both minisatellites and SSRs can be used to genetically distinguish the analysed materials. Furthermore, these two marker systems depict relationships consistent with the hierarchal pattern obtained by the morphological data. As locally cultivated tomato accessions are often characterised by some degree of genetic variability, our results will be valuable in facilitating the purification, management and breeding of tomato germplasms. The differences between the marker systems employed are also discussed in relation to their usefulness in the agro-food chain.     

Agronomic traits of soybean cultivars released in different decades after grafting record‐yield cultivar as rootstock

Breeders have seldom considered the selection for root traits during the genetic improvement in soybean. It is hypothesized that grain yield would be increased by the root function improvement, especially for the current cultivars. The objective of this grafting experiment was to determine the effect of record‐yield cultivars L14 or Z35 as rootstocks on agronomic traits of cultivars released in different decades. A total of 11 cultivars, released in different decades, were used to graft onto L14 or Z35 rootstocks. The agronomic traits were measured in the pot‐culture experiments. Grafting cultivars released in different decades onto L14 or Z35 rootstocks resulted in higher yield, 100‐seed mass and apparent harvest index as compared with those of non‐grafted or self‐grafted plants. Grain yield gain of cultivars grafted onto record‐yield cultivar rootstocks was 0.40 g/plant/year from 1966 to 2006, which was larger than that of non‐grafts and self‐grafts (0.27 g/plant/year). The yield of current cultivars should increase more if their root functions were improved.     

Frequency and fertility restoration efficiency of Rf3 and Rf4 genes in Indian rice

Identification of new parental lines is crucial for developing ecology‐specific hybrids with ideal agronomic performance. We screened a total of 570 different ecology‐specific Indian rice varieties for the presence of fertility restorer genes, Rf3 and Rf4 using tightly linked markers DRRM Rf3‐10 and RM6100, respectively. Among these varieties, 13% carried Rf3Rf3/Rf4Rf4, 31% carried rf3rf3/rf4rf4, 6% carried Rf3Rf3/rf4rf4 and remaining 50% carried Rf4Rf4/rf3rf3 allelic combinations. A mini set of 40 varieties with variable allelic combinations of fertility restorer genes were testcrossed with WA and Kalinga‐based CMS lines. All the 80 F₁s were evaluated for spikelet fertility and fertility restoration ability. Rf3Rf3/rf4rf4 genotypes mostly behaved as partial maintainers or partial restorers. In contrast, rf3rf3/Rf4Rf4 genotypes were partial or effective restorers. However, double dominant genotypes showed better fertility restoration than the genotypes containing Rf3 or Rf4 individually. Some of the genotypes showed unexpected restoration pattern implying occurrence of other fertility restorer(s) apart from Rf3 and Rf4. The perfect restorers and maintainers identified in this study can be directly used in hybrid rice breeding.     

Uneven ripening in ‘Bangalore blue’ grape (Vitis vinifera × Vitis labrusca) berries in India is linked to seed viability

Uneven ripening (UR) is a physiological disorder of unknown origin in ‘Bangalore blue’ grape (Vitis vinifera × Vitis labrusca) leading to wine of inferior quality. A preliminary study found wide variations in total dehydrogenase activity (TDH) of seeds from unevenly ripe berries. In our experiments, gibberellin (GA₃) applied to young grapes increased seed TDH activity and reduced the incidence of uneven ripening to 2% compared with 35% in the control. In contrast, paclobutrazol (PBZ) decreased TDH activity and increased the incidence of the disorder to 58%. GA₃-treated berries had higher concentrations of sucrose and TDH activity in seed representing mature seeds with high viability. In contrast, PBZ-treated and control berries had higher concentrations of glucose and lower TDH activity, indicating immature seeds with low viability. These results suggested that competition among developing berries can lead to differences in seed gibberellin content, seed viability and the rate of berry growth resulting in green, purple, and black berries at harvest. The study established the role of seed viability in uneven ripening and demonstrated that the incidence of the disorder is reduced by the application of GA₃ to immature berries.     

基于ADAMS的油茶果采摘机械臂试验设计及优化

针对目前我国油茶采摘主要以人工为主,劳动强度大、采摘效率低等问题,设计了一种适合园艺化栽培的履带式油茶果采摘机,并在Adams软件中对机械臂进行试验设计和优化仿真。试验数据表明:油茶果采摘机工作区间与各机械臂的长度和液压缸安装距离呈线性相关,最佳组合处的机械臂长度总和减小25.67%,可为油茶果采摘机的机械臂设计提供参考。     

基于高光谱图像技术预测苹果大小

以新疆红富士苹果为研究对象,探讨应用高光谱图像技术和最小外接矩形法预测其大小的研究方法。提取苹果高光谱图像中可见红色区域受色度影响较小的713nm以及近红外区域793和852nm的3个波长图像,做双波段比运算处理。比较所得双波段比图像可知,852/713双波段比图像中背景和前景灰度对比度最大。对该图像做阈值分割以及形态闭运算去除果梗区域,使用8邻接边界跟踪法得到二值图像的轮廓坐标序列,采用最小外接矩形法求苹果的大小,与实测值建立回归方程。结果表明,基于高光谱图像技术采用波段比算法,结合最小外接矩形法,能够有效地检测苹果大小,预测值与实际值最大绝对误差为3.06mm,均方根误差为1.21mm。     

切-双纵流脱分装置试验研究与分析

阐述了切-双纵轴流脱粒分离装置的结构组成及工作原理,基于切-双纵流联合收获机,以喂入量、切流滚筒间隙及滚筒转速为影响因子,脱粒破碎率及脱粒损失率为指标,进行田间性能试验,并利用极差分析分别对破碎率、脱粒损失率单影响因子进行分析。结果表明:对脱粒破碎率影响的主次因素为BCA,即切流滚筒转速纵轴流滚筒转速喂入量,最佳组合为切流滚筒间隙25/30mm、滚筒转速907/1 043r/min、喂入量10.2 kg/s;对脱粒损失率影响的主次因素为BAC,即切流滚筒间隙喂入量滚筒转速,最佳组合为切流滚筒间隙25/30mm、喂入量10.2kg/s、滚筒转速953/1 096r/min。     

苹果图像的背景分割与目标提取

水果的缺陷、大小和颜色差异以及光照等因素影响图像背景分割与目标提取精度.以苹果为研究对象,针对4种不同光照强度条件下采集的280幅不同姿态的苹果图像,将彩色图像的R、G、B分量进行算术运算,然后采用形态学开运算进行消噪处理,采用线性空间滤波消除锯齿状边界,采用自动阈值分割方法进行背景分割与目标提取.结果显示,203幅图像的分割偏差小于1％,占总量的72.5％;70幅图像的分割偏差大于1％而小于2％,占总量的25％;偏差大于2％的有7幅,占总量的2.5％.最大分割偏差为2.83％.     

Interaction of curcumin with phosphatidylcholine: A spectrofluorometric study

Curcumin [1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3, 5-dione], the main constituent of the rhizomes of the plant Curcume longa L. (turmeric), is a powerful antioxidant in both enzymatic and nonenzymatic systems. The interactions of curcumin with egg and soy phosphatidylcholine were followed by fluorescence spectroscopy. Curcumin had very weak fluorescence in aqueous system, which was enhanced in apolar environments. Curcumin emitted at 490 nm after being excited at 451 nm in phosphatidylcholine micelles. The equilibrium constants for the interaction of curcumin with egg and soy phosphatidylcholine were (3.26 +/- 0.2) x 10(5) and (2.64 +/- 0.2) x 10(5) M(-1), respectively. From the Scatchard plot of the fluorometric data, it was inferred that one molecule of curcumin could bind six molecules of phosphatidylcholine. The equilibrium constant for the phosphatidylcholine-curcumin interaction decreased with temperature, indicating the amphiphilic nature of curcumin. The DeltaG, DeltaH, and DeltaS values obtained for the interaction of egg phosphatidylcholine-curcumin were -7.8 +/- 0.3 kcal/mol, -9.6 +/- 0.4 kcal/mol, and -6.8 +/- 0.2 cal/mol/K, respectively. The fluorescence anisotropy measurements of curcumin with phosphatidylcholine suggested that the anisotropy of the curcumin molecule did not change in phosphatidylcholine. The interaction of divalent metal ions with phosphatidylcholine-curcumin in comparison with phosphatidylcholine-1-anilino-8-naphathalenesulfonic acid complex suggested the strong binding of curcumin to metal ions.