巫溪红池坝改良草地鼠类调查报告

对重庆巫溪红池坝改良草地中鼠形动物的种类进行抽样调查、室内饲养、解剖鼠胃和洞道研究，结果显示，该改良草地中有鼠形动物2目3科4种，其对草地危害方式和为害程度均不一样。提出了改良草地中鼠害的防治措施。     

5个草地型草地牧草的生长速率和养分动态研究

在牧草生长季节(5~9月)对小哨牧场放牧演替形成的5个草地型草地的牧草进行生长速率、牧草产量及养分的逐月观测,生长速率测定显示,白三叶+东非狼尾草和东非狼尾草+非洲狗尾草两个草地型草地均出现两个生长峰值,分别在6月和9月,其余3个草地型草地只有一个生长高峰。干物质产量和牧草养分测定结果表明,白三叶+东非狼尾草和白三叶+非洲狗尾草+画眉草两种草地型草地表现最好,可以在气候环境条件类似的地区推广种植利用;白三叶+画眉草和白三叶+非洲狗尾草两种草地型草地表现优良,可以在草地改良中,根据当地实际情况选择性地加以种植;东非狼尾草+非洲狗尾草型草地表现较差,不提倡种植。     

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AIM: To investigate the contribution of angiotensin-converting enzyme inhibitor (ACEI) to the regulation of calpain system in infarcted myocardium. METHODS: Rat myocardial infarction (MI) model was established by permanent ligation of the left coronary artery. The treatment with the ACEI inhibitor rampril (1 mg·kg-1·d-1) was started 7 days prior to surgery. On day 1, 3, 7 and 14 after MI, protein levels of calpainⅠ, Ⅱ and calpastatin were determined in left ventricular free wall (LVFW), interventricular septum (IS) and right ventricule. RESULTS: CalpainⅠprotein level was increased in IS 14 d post MI, whereas the protein level of calpainⅡ was maximally increased in LVFW 3 d post MI. Rampril decreased protein up-regulation of calpainⅠ and Ⅱ, and reduced infarct size and interstitial fibrosis. Calpastatin protein expression was not affected by ACEI. CONCLUSIONS: CalpainⅠ is involved in cardiac remodelling in the late and calpainⅡ contributes to cardiac tissue damage in the early phase of MI. The heart protective effect of ACEI may be related to the inhibition of calpain system in the pathogenesis of myocardial infarction.     

绵阳城市广场建设中的特色分析

在调查的基础上，介绍了四川绵阳市的广场，并以铁牛广场为例分析其特色和不足。     

母猪卧向行为学的观察

本文通过2个试验对母猪在分娩圈和分娩栏中的卧向行为进行了观察,试验一中, 选择10头大白(Yorkshire)经产母猪作为观察对象,试验二中分别选择10头大白(Yorkshire) 经产母猪和10头长白(Landrace)经产母猪作为观察对象。观察采用瞬时记录方法,每周观察 三次,隔日观察,每天上午、下午各观察一次,每次3 h,每次观察间隔5 min。观察中发现,母猪 卧向以向外为主。分娩栏(四周是栏杆)中的母猪以选择卧向外(向北)为主,其次是卧向内(向 南),卧向左和卧向右最少且差异不显著,上栏前和下栏后差异不显著。分娩圈(四周是墙壁)中 母猪以选择卧向外为主,卧向内最少,卧向左和卧向右差异不显著。长白母猪比大白母猪选择 卧向外的多,妊娠阶段比哺乳阶段选择卧向外的多。     

Selection and amplification of the liver stem cell subset from rat bone marrow cells with a medium containing cholestatic serum in vitro

AIM: To explore the feasibility of direct separat and selective enlargement of the bone marrow-derived liver stem cells (BDLSC) from bone marrow cells with a culture system containing cholestatic serum in vitro. METHODS: Bone marrow cells of rats were cultured with selective media containing 2%, 5%, 7% and 10% cholestatic rat serum, respectively. The BDLSC were then induced to proliferate with the addition of hepatocyte growth factor (HGF) on the firth day. BDLSC were characterized using immunocytochemistry and RT-PCR for lineage markers, glycogen staining and urea synthetic assay for functions 2 weeks later. RESULTS: Bone marrow cells were unble to form colony in the presence of 2% cholestatic serum and apopotosis appeared gradually in 7% or 10% cholestatic serum. The BDLSC survived in the medium containing 5% cholestatic serum while the other types of cells did not. The survival cells proliferated with a high speed during the second week and then formed hepatocyte-like colony-forming units (H-CFU). Cells in the H-CFU expressed the characteristic proteins of fetal hepatocytes. Furthermore, they had glycogen storage and urea synthesis functions, two of the critical features of hepatocytes. CONCLUSION: The selective micro-environment effectively selected BDLSC from the bone marrow cell, and will be a new way to provide an abundant source of donor hepatocytes for clinical cell therapy.     

Effect of mutant β-catenin gene on the proliferation of hepatocytes

AIM:The β-catenin is a key molecule in the Wnt signal pathway, which plays a critical role in normal development and tumorigenesis. However, the mechanisms of the β-catenin on the cell growth control are still not completely defined. The aim of this study was to test the hypothesis that the mutant β-catenin may regulate the hepatocyte proliferation. METHODS: The immortalized murine hepatocyte cell line, AML12, was used for this study. A plasmid that contain mutant β-catenin S33Y was transfected into the AML12 cells and a stable cell line AML12S33Y was established. The cell growth property of this cell line and the parental cell were compared by flow cytometry analysis and direct cell count. The cells were also tested for the ability to form soft agar colonies, and the ability to form tumors in the severe immune deficient mice (SCID). RESULTS:1. The mutant β-catenin containing cell line AML12S33Y has higher proliferating index compared with the parental AML12 cells (P<0.01), suggesting that mutant β-catenin promotes cell growth. 2. The mutant β-catenin cells formed small colonies in soft agar after 4 weeks of culture, but did not generate tumor in SCID mice. CONCLUSION:The mutant β-catenin promotes liver cell growth.     

区域旅游资源开发定位研究——以内蒙古赤峰市为例

区域旅游资源开发是发展区域旅游的基础 ,开发的定位研究有助于为一地旅游业的发展指明方向。本文从区域角度和空间经济一体化思想出发 ,以赤峰市为例 ,通过对旅游资源的定量与定性研究 ,得到旅游资源的综合评价图式 ,结合区际分析归纳出赤峰市旅游资源开发利用的方向和层次。从而为区域旅游开发的定位研究进行了一般理论的探讨及例证分析     

A reactive pattern of ischemia-induced nestin protein in the rat brain

AIM:To explore the expressive profile of nestin protein in the focal ischemic brain and to study the recovery mechanism of brain focal infarct.METHODS:Cellular morphology,time-course and distribution pattern of nestin positive response were immunohistochemically examined in different brain regions of 36 adult male SD rats. RESULTS:Nestin positive response of different brain regions in sham operated rats was present in small- and micro-vasculartures and the third ventricle bottom and ependyma. A large number of nestin positive cells were detected in ischemic brain, and were more remarkable in the cortical areas of parietal lobe and preoptic area as well as ischemic caudoputamen. Stellate nestin positive cells were located in the deep layer of ischemic cortex, but fibrillary cells were located in the shallow layer. Nestin positive cells in the ischemic caudoputamen showed the same changes of morphology as those cells in the deep layer of ischemic cortex. Morphological and number alterations of nestin positive cells were the most remarkable at 1 weeks post-ischemia, which showed more hypertrophy and proliferation in morphology, and a marked increase in number was present in the ischemic cerebral cortex and the ischemic caudoputamen. These alterations of nestin positive cells persisted up to 6 weeks post-ischemia, and then, the nestin positive response in the ischemic brain decreased gradually.CONCLUSION:Focal cerebral ischemia induces nestin re-expression on reactive astrocytes, which may be very important to the self-recovery of cerebral infarct.     

果梅幼树对春施~(15)N-硫铵的吸收与分配

以盆栽3年生细叶青梅/毛桃为试材,研究了早春施用~(15)N-(NH_4)_2SO_4条件下,果梅对~(15)N的吸收分配规律。结果表明:由于春季土温较低,限制了植株对肥料氮的利用率。在新梢旺长期,植株从肥料氮中吸收的氮素营养主要用于新生器官的建造,且新梢成为~(15)N的主要分配中心,其次即为果实,再其次为细根。至花芽分化期,植株的生长中心已发生转移和分散,但春施氮对促进当年生枝的花芽分化和维持叶片正常光合功能仍有重要作用,此期亦是根系生长的关键时期之一,且与贮氮相比,春施氮更有利于当年新根的萌发和根系的扩大。