Effect of L-Arg on plasma content of endothelin and expression of c-fos mRNA in the left ventricle of rats with renovascular hypertensive hypertrophy

AIM:To study the effect of L-Arg on plasma content of endothelin (ET) and the expression of proto-oncogene c-fos mRNA in the left ventricle of rats with renovascular hypertensive hypertrophy. METHODS: The level of c-fos mRNA were measured by in situ hybridization. The ET in plasma were measured by radioimmunoassay. RESULTS:After eight weeks of treatment with L-Arg, the expression of c-fos decreased markedly (P＜0.01). The ET content in plasma also decreased significantly by L-Arg(P＜0.01).CONCLUSION: Plasma ET content and the expression of c-fos in the left ventricle of rats with renovascular hypertensive hypertrophy could be decreased by L-Arg administration.     

The Effect of Caffeic Acid on Zearalenone-induced Ovarian Granulosa Cell Apoptosis in Mice

Ovarian granulosa cells provide a special microenvironment for follicle formation and maturation through interaction with oocytes and their own secretion. A variety of harmful stimuli can cause granulosa cell apoptosis and metabolic disorders, reduce the quality of oocytes and have a negative impact on embryo formation. Zearalenone (ZEA) is a common cause of ovarian granulosa cells injury in the livestock industry, which is produced by mycotoxins, and lack of effective treatment drug. Therefore, in the current study zearalenone was used to induce ovarian granulosa cell injury and to explore the protective effect of caffeic acid on zearalenone-induced ovarian granulosa cell apoptosis in mice. Mouse ovarian granulosa cells were isolated by mechanical method, and indirect immunofluorescence was used to identify the isolated cells. MTT assay was used to determine the effect of caffeic acid on the activity of normal mouse ovarian granulosa cells.After granulosa cells were co-treated with caffeic acid (200, 100 and 50 μg·mL^-1) and ZEA for 24 hours, and control and ZEA group were set up at the same time, cell morphology and adherence were observed under a microscope. MTT was also used to detect cell viability. Caspase-3 mRNA expression level was detected by qRT-PCR. Cleaved-caspase-3 and cleaved-PARP protein expre-ssion levels were determined by Western blot. The results showed that positive FSHR staining appeared in cell cytoplasm of the test group, which confirmed that the isolated cells were mouse ovarian granulosa cells. The cell viability was above 90% which showed that caffeic acid had no toxic effect on granulosa cells. Compared with control group, ZEA group had smaller cell size, poor adherence, increased cell gap, and significant reduction in cell viability (P<0.001). Furthermore, the relative expression of caspase-3 mRNA, and cleaved-caspase-3 and cleaved-PARP protein level were significantly increased (P<0.001) compared with the control group. After caffeic acid treatment, cell gap was reduced, adherence was tight, cell viability was significantly increased (P<0.001). Caffeic acid significantly reduced zearalenone-induced increase in caspase-3 mRNA, and cleaved-caspase-3 and cleaved-PARP protein expression level (P<0.001). This study indicated that caffeic acid can restore granulosa cell viability by inhibiting ZEA-induced apoptosis.     

“香两优98049”的选育与应用

用“香125S”与“98049”进行杂交,育成早稻新组合“香两优98049”。该组合产量高、抗性强。简要介绍了该组合的栽培和制种技术要点。     

浑善达克沙地动态变化影响因素分析

通过景观的角度分析该区的动态变化影响因素可知,气温升高和降水量减少是荒 漠化景观发展的基础和背景条件,超载过牧是荒漠化景观发展的主要原因,至于其他因素如滥 砍、滥伐、滥垦、滥挖药材、搂发菜、樵采等起了推波助澜的作用。     

浙江富阳金属冶炼厂周围水田土壤-水稻系统中As、Se含量特征

本文研究了浙江富阳金属冶炼厂周围水田土壤-水稻系统中As、Se含量特征。所采土壤样品中,As含量高于土壤环境质量一、二和三级标准的分别占总数的21.15%、9.62%和7.69%。该金属冶炼厂周围88.46%的土壤属于富Se土壤。足Se土壤占总样的5.77%,过量Se土壤占3.85%,高Se土壤占5.77%。水田土壤可能受与金属冶炼有关的点源污染影响。糙米的As、Se含量高于全国糙米As、Se背景值,可食部分As、Se浓度均未超过我国规定的水稻卫生标准。     

2个多年生花生品种低温胁迫下活性氧代谢特性研究

不同低温胁迫下对平托花生Arachis pintoi与蔓花生A.duranensis2个多年生花生品种进行的叶片活性氧代谢与质膜特性进行研究。结果表明,A.duranensis品种无论在超氧化物岐化酶(SOD)、过氧化物酶(POD)还是过氧化氢酶(CAT)相对活性在低温胁迫下均不低于或显著高于A.pintoi,表现出相对较高的清除自由基能力;其质膜相对透性(RPP)增加幅度明显小于A.pintoi;丙二醛(MDA)相对含量增加率则表现出相反趋势;表明A.duranensis具有更强的抗寒性。     

野生牧草金荞麦与贵州省推广牧草栽培效益比较试验初报

金荞麦Poyigonum cymosum是一种药饲兼用的贵州野生植物资源,通过金荞麦与贵州省目前主要推广牧草的田间试验比较,研究金荞麦和推广牧草资源的产量、品质和饲用的不同特点。试验初步认为:金荞麦是一种高产优质的牧草资源,其产量与菊苣Cichorium intybus相似,比一般推广牧草如苜蓿Medicagosativa等高出2~3倍。其粗蛋白含量与菊苣相似,接近苜蓿。金荞麦的适口性好,耐瘠抗劣性强,易繁殖成活,有较大的推广潜力。     

施氮量对盐角草生长及钠离子累积的影响

为了研究施氮对盐角草生长及其钠离子累积的影响,本研究通过5个氮肥水平大田小区试验分析了不同施氮量对盐角草生长和钠离子累积的影响.结果表明:施氮能够显著增加盐角草茎秆、籽粒和同化枝的产量;在0 ～ 600 kg/hm2范围内,盐角草的生物量及籽粒产量随着施氮量增加而呈现直线增长的趋势.施氮对盐角草茎秆、籽粒和同化枝中的Na+含量无显著影响,但盐角草同化枝中的Na+含量显著高于茎秆和籽粒.施氮能够促进盐角草各部分对Na+的累积,600 kg/hm2的施氮量条件下,盐角草的Na+累积量平均达到了9668 kg/hm2,较不施氮的2045 kg/hm2增加了370％.因此,本研究表明,施氮能够促进盐角草对盐渍土的改良效果.