Vitamin C enhanced myocardial differentiation of dedifferentiated fat cells

AIM: In order to observe the myocardial differentiation capacity of the dedifferentiated fat (DFAT) cells treated with vitamin C in vitro. METHODS: DFAT cells were dedifferentiated from the mature rat adipocytes with ceiling adherent culture. The DFAT cells of passage 3 were used in the study. Vitamin C and/or neonatal rat heart tissue lysate were added into the culture medium to induce myocardial differentiation for 3 weeks. The cell morphology was observed under microscope. The myocardial-specific markers, such as cTnT, GATA-4 and NKx2.5, were examined by the methods of immunofluorescence, PCR and Western blot. RESULTS: Mature rat adipocytes dedifferentiated into fibroblast-like DFAT cells after ceiling adherent culture. The DFAT cells spontaneously differentiated into cardiomyocyte-like cells under normal culture condition with a low incidence. After treated with neonatal rat heart cell lysate, the DFAT cells became cardiomyocyte-like cells that had bigger size, longer shape and myotubule-structure. The expression of cTnT, GATA-4 and NKx2.5 was remarkably increased at both mRNA and protein levels as compared with the normal cultured DFAT cells. The expression of cTnT, GATA-4 and NKx2.5 was further increased in DFAT cells after treating with vitamin C. No spontaneous beating cell was observed. CONCLUSION: Vitamin C enhances the differentiation of DFAT cells into cardiomyocyte-like cells.     

Preliminary Study on NF-κB Signaling Pathway Regulating Brucella Intracellular Survival Molecular Mechanisms

By the infection of Brucella virulent strain and attenuated strain in mice macrophage RAW264.7,the assay was aimed to explore the relationship between NF-κB signaling pathways and Brucella virulent strain and attenuated strain in intracellular survival.Use different MOI Brucella (2308,RB51,16M and M5) to infect mice macrophage RAW264.7,after 0,4,8 and 24 h infected,cracking cell and collecting supernatant,we detected the effect of Brucella on activation of NF-κB signaling pathway by Western blotting.Different concentrations of NF-κB signaling pathway inhibitor were incubated with mice macrophage RAW264.7,with different multiplicities of infection (MOI) of Brucella infecting cells,ELISA kits to detect the expressions of TNF-α,IL-1β and IL-6 cytokine;At the same time,count the number of intracellular bacteria of CFU.The results showed that rough cattle Brucella strains RB51 could strongly activate NF-κB signaling pathway,smooth cattle Brucella strains 2308 was weak in the activation;At the same time,the activation of NF-κB signaling pathway was concentration dependent.When the MOI was 80,infection time was 8 h,NF-κB activation degrees of rough cattle Brucella strains RB51 and smooth cattle Brucella strains 2308 were the strongest,and this pathway was involved in producing TNF-α and IL-6;NF-κB signaling pathway inhibitor BAY11-7082 affected Brucella intracellular survival.So rough cattle Brucella strains RB51 intracellular survival and NF-κB signaling pathway activity were closely related.The results laid the foundation for the further study of Brucella intracellular pathogenesis,also provided scientific basis for the research of new drugs to Brucella,and prevention and treatment of brucellosis.     

K步稳定的鞋印花纹图像自动聚类

目的 鞋印是刑事侦查的重要物证之一,如何对积累的大量鞋印花纹图像进行自动归类管理是刑事技术迫切需要解决的问题之一。与其他类图像不同,鞋印花纹图像具有种类多但数目未知、同类花纹分布不均匀且同类花纹数目少的特点。基于鞋印花纹图像的这些特点,用目前典型的聚类算法对鞋印花纹图像集进行聚类,并不能取得很好的效果。在对鞋印花纹图像进行分析的基础上,提出一种K步稳定的鞋印花纹图像自动聚类算法。方法 对已标记的鞋印花纹图像进行统计发现,各类鞋印花纹之间在特征空间上存在互不相交的区域(本文称为隔离带)。算法的核心思想是寻找各类鞋印花纹之间的隔离带,来将各类分开。过程为:以单调递增或递减的方式调整特征空间中判定两点为一类的阈值,得到数据集的多次划分;若在连续K次划分的过程中,某一类的成员不发生变化,则说明这K次调整是在隔离带中进行的,即聚出一类,并从数据集中删除已标记的数据;选择下一个阈值对剩余的数据集进行划分,输出K步不变的类;依此类推,直到剩余数据集为空,聚类完成。结果 在两类公开测试数据集和实际鞋印花纹数据集上进行实验,本文算法的主要性能指标都超过典型算法,其中在包含5792枚实际鞋印花纹数据集上的聚类准确率和F-Measure值分别达到了99.68%和95.99%。结论 针对鞋印花纹图像特点,提出了一种通过寻找各类之间的隔离带进行自动聚类的算法,并在实际应用中取得了很好的效果。且算法性能受参数的变化以及类的形状影响较小。本文算法同样适用于具有类似特点的其他数据集的自动聚类。     

农业科研单位编制外用工的管理策略

编制外用工是农业科研单位普遍存在的用工形式。本文阐述了农业科研单位编制外用工的特点，分析了编制外用工存在问题及原因，提出从规范用工管理方式、强化考核管理、拓宽发展空间等方面提高编制外人员的工作积极性，提升编制外用工人力资源管理的效能，促进单位发展。     

Wx^mp基因背景下可溶性淀粉合成酶基因SSⅡa和去分支酶基因PUL对水稻蒸煮食味品质的影响

【目的】分析在同一主效基因(Wx^mp)背景下可溶性淀粉合成酶基因SSⅡa和去分支酶基因PUL对稻米蒸煮食味品质的影响,以期为水稻品质遗传改良提供依据。【方法】选择在SSⅡa和PUL存在多态性而其他淀粉合成酶相关基因没有多态性的半糯品系宁0145和粳稻品种武运粳21进行杂交,获得F2群体与F3株系。利用分子标记,选择含有Wx^mp基因的F2单株与F3株系,将这些F2单株与F3株系分成SSⅡa^nPUL^n、SSⅡa^nPUL^w、SSⅡa^wPUL^n和SSⅡa^wPUL^w4种基因型(n和w分别表示该基因来源于宁0145和武运粳21),分析不同基因型蒸煮食味品质性状的差异,探讨同一Wxmp基因背景下不同SSⅡa和PUL等位基因对蒸煮食味品质性状的影响。【结果】不同基因型间蒸煮食味品质性状均存在显著差异,来源于武运粳21的SSⅡa^w基因和PUL^w基因分别使直链淀粉含量增加0.29%~1.00%和0.62%~1.18%,且PUL的效应大于SSⅡa,两者间存在互作效应。SSⅡa^w基因和PUL^w基因降低胶稠度和崩解值,提高了热浆黏度、冷胶黏度、消减值和回复值,对糊化温度、峰值黏度和峰值时间的作用较小。【结论】明确了Wx^mp背景下SSⅡa和PUL基因对稻米蒸煮食味品质的遗传效应,该研究结果为SSⅡa和PUL基因的分子标记辅助选择改良稻米品质提供了理论依据。     

北京小西山山前洪积扇不同土地利用方式下的土壤微形态特征

通过对北京小西山山前代表性洪积扇的观察研究,探索不同土地利用方式对土壤微形态特征的影响。通过对土壤薄片的观察,分析土壤微形态特征;用ENVI及MATLAB软件处理图片,并对孔隙度进行定量分析。结果表明:1)林地(WL1)剖面表层土壤微结构为海绵状结构,而园地(WL2)和菜地(WL3)的表层土壤多呈整块状结构或孔道结构;2)各采样点土壤矿物成分相近,但骨骼颗粒形态特征有较大差异。3)WL1剖面土壤孔隙度较高,以面状孔隙为主,WL2和WL3剖面土壤孔隙度过低,以中小孔隙居多。研究认为:1)林地的土壤微结构较园地和菜地的好,由于人类的不合理利用和管理,园地和菜地使土壤微结构变差,土壤孔隙度下降。2)各采样点土壤成土母质相似,土壤骨骼颗粒形态差异受到不同土地利用方式下人类干扰程度差异的影响。     

南方双季稻区小行距插秧机的改进设计与试验

针对300mm行距插秧机在南方双季稻区栽插行距过大、穴数过少及不能充分发挥机插稻的增产潜力问题,在300mm行距插秧机结构的基础上,通过机械优化设计和田间农艺技术相结合的方法,对其插植部箱体、苗箱、支架(包括左右支架和中间支架)和浮板4个关键部位技术参数改进设计,研制出了适合南方双季稻区作业的2ZS-488B型(264mm行距)插秧机,并于2012-2013年在江西省2个试验点进行不同早晚稻品种机插行距的大田对比试验。结果表明:该插秧机作业性能稳定,漂秧率、伤秧率和漏插率分别为1.55%、2.68%和1.93%,相对均匀度合格率为89.54%。与300mm行距插秧机相比,其产量平均增产529.43kg/hm2,平均增产率达8.57%。该研究结果创新了南方双季稻区新的机械化栽插方式,有利于农机农艺的有效融合。     

Molecular Cloning,Sequence Analysis and Tissues Expression Profile of Part of Mink Toll-like Receptors (TLRs) Genes

The present study was aimed to explore the potential role of Toll-like receptors (TLRs) on the mink immunity, and accumulate alternative genetic material for the mink breeding for disease-resistant.Four pairs of primers were designed according to ferret TLR sequences in GenBank to obtain the sequences of TLR genes (TLR4, TLR6, TLR7 and TLR8) of mink and then performed bioinformatics analysis of these sequences.In addition, the expression of TLR4 and TLR7 genes in various tissues in young and adult minks were analyzed by RT-PCR. Sequence homology analysis showed that mink had higher homology with carnivora (ferrets, polar bear, panda, walrus, seals, dog, tiger and cat) which had the nearest perimeter in the phylogenetic tree.Tissue expression analysis showed that TLR4 and TLR7 were widely and differently expressed in variety tissues of mink.Interestingly, the expression of TLR4 and TLR7 in young mink were much higher than that in adult mink.