安徽省鸡常见病毒性肿瘤病的检测与调查

应用已建立的鸡肿瘤病快速鉴别诊断技术对源自安徽省5个不同地区的46个鸡群的肿瘤病、抑制病疑似病、死鸡305羽进行检测。结果表明,马立克氏病的阳性率为39．34%(120/305),禽网状内皮组织增生征阳性率为8．52%(26/305),禽白血病阳性率为2．624%(8/305)；另对17群未进行MD免疫的肉鸡进行随机抽样检测,其中5个鸡群检测出马立克氏病病毒强毒,阳性率为29．41%(5/17),阳性群的平均检出率为24 %。     

鸭瘟病毒的PCR检测及其产物分析

根据基因库中已有的鸭瘟 Ul6的序列 ,合成一对引物 ,对 3株鸭瘟病毒进行 PCR扩增 ,均扩增出大小约 42 0 bp的特异性片段。进行测序 ,结果表明 3个毒株扩增后的片段均为 42 1bp。经重复实验后确定最佳反应条件 ,按该条件对两例临床病料进行检测 ,结果均出现大小约为 42 0 bp的特异片段。PCR产物与 L ake Andes株的相关序列比较 ,广东毒株与 L ake Andes株同源性较高     

执行中止制度的改革与完善

在执行实务中,由于立法上的缺陷和执行中止的随意性太大,过多的适用中止执行导致了大量的执行积案,使执行法院背上了“包袱”;同时,现行的执行中止制度承载了执行当事人过多的误解和不满。本文从执行中止事由的纯化、执行观念的更新和债权凭证制度的构建等方面为建立新的执行中止制度提出了具体的设想。     

马传贫驴白细胞弱毒疫苗株跨膜蛋白主要免疫决定区基因的克隆与表达

从感染驴白细胞的马传贫驴白细胞弱毒疫苗株前病毒DNA中克隆了编码跨膜蛋白主要免疫决定区(TMIR)的基因，并在大肠杆菌中进行了表达。所表达的融合蛋白有一部分是可溶的，其氨基端带有6个组氨酸的标签，因此可以用固定化金属离子亲和层析法在非变性条件下进行纯化。在间接酶联免疫吸附试验(ELISA)和免疫印迹试验中，重组的TMIR蛋白可与马传贫阳性血清样品发生反应，而与健康马血清无任何反应。这表明该重组蛋白具有良好的抗原性和特异性，可用于马传贫弱毒疫苗株在体内外复制、接种马体内免疫应答及马传贫诊断的研究。     

不同放牧强度对滩羊生产性能影响的研究

为寻找适宜宁夏盐池县草地的放牧强度,于2003年5-11月在该县四墩子试点设5个处理(0.45、0.60、1.75、1.05和1.50只/hm2)进行轮牧,轮牧周期为42 d,共放牧154 d。结果表明:1)滩羊采食量随放牧强度的加重而降低。同一放牧强度下,随放牧时间推移,采食量先逐渐增大而后下降。2)在试验期内,不同放牧强度下,滩羊体重随时间推移总体上都呈增加趋势。在10月2日之后体重开始出现分化。与放牧强度重的处理相比,放牧强度轻的处理日增重峰值较高,持续时间较长。3)滩羊个体增重与放牧强度之间存在着强的负相关;单位草地面积(1 hm2)增重与放牧强度之间呈强的正相关,初步可以确定在该类草地上放牧强度应在0.75只/hm2左右。4)随着放牧强度的加重,饲料报酬先增大后减小。在同一放牧强度下,饲料报酬随时间的推移先升高后降低。5)当放牧强度超过0.75只/hm2以后,滩羊出现了空怀、产羔率降低和推迟怀孕的现象。6)综合考虑各研究指标,该类草地放牧强度以不应超过0.75只/hm2为宜。     

利用看不见网站获取网上学术信息资源

阐述了看不见网站的涵义和类型，探讨了如何利用看不见网站获取网上学术信息资源。     

春小麦新品种互麦14号栽培技术

介绍了我省育成的春小麦新品种—互麦14号的丰产栽培技术。     

Spermatophore cryopreservation and artificial insemination of black tiger shrimp, Penaeus monodon (Fabricius)

To develop an appropriate cryopreservation protocol for spermatophores of black tiger shrimp, Penaeus monodon, three cryoprotectants (dimethyl sulphoxide (DMSO), methanol (MeOH) and ethylene glycol (EG)) at two concentrations (5% and 10%) were examined. Artificial implantation of spermatophores was also carried out to assess the fertilizing ability of fresh and post‐thaw spermatophores. Spermatophores were collected during consecutive regenerations (15‐day intervals) and assessed for qualitative and quantitative changes and also for fertilizing ability by implantation. The mean fertilization rate for artificial insemination using post‐thaw spermatophore was 79.9±3.7%, lower than the fertilization rates observed for artificial implantation using fresh spermatophore and natural mating. Mean hatch rates for fresh spermatophore, frozen‐thawed spermatophore and natural mating were 88.8±0.6%, 87.8±0.4% and 88.3±0.5%, respectively; and there was no difference among the three groups. The mean fertilization rate of spermatophores collected during the first stripping was higher (90.6±0.6) than during the second stripping (85.7±2.6), but the mean hatch rate was not different between the two strippings. The highest mean sperm viability (79.7±0.4%) was obtained from DMSO (5%), with no survival observed in the 10% MeOH treatment. Spermatophore weight, total sperm count and percentage of abnormal sperm were not different between spermatophores collected at the first and second stripping. This is the first study to report high fertilization and hatch rates from cryopreserved spermatophore using artificial implantation of spermatophore before spawning.     

利用PCR对鸭瘟和鸭"传染性肿头症"的鉴别诊断

本文利用聚合链反应(PCR)技术对鸭瘟和鸭“传染性肿头症”进行鉴别诊断。结果证明该方法具有特异、快速的特点。可对这两种临床症状较为相似的鸭传染性疾病进行快速鉴别诊断。     

An evaluation of the pesera soil erosion model and its application to a case study in Zakynthos, Greece

Abstract. The Pan‐European Soil Erosion Risk Assessment (pesera ) model was evaluated using existing soil erosion data collected under various types of climate, vegetation, landscape and soil conditions. The data used represent a variety of typical Mediterranean land uses such as winter wheat, vines, olives and bare, stony land prevailing in hilly areas. Using this data, the model was calibrated for sediment transport by overland flow and results compared to measured soil erosion values from runoff plots and a watershed on a monthly basis. The performance of the model was assessed statistically, showing that it can be satisfactorily used for predicting soil erosion rates under the conditions included in the study. The overall model estimate including all the available experimental data was 0.69 t ha^?1 yr^?1 with a maximum error of 1.49 t ha^?1 yr^?1. After validation, the model was applied to a small watershed (60 ha) of great ecological importance for the sea turtle Caretta caretta. For this purpose, soil and vegetation maps were compiled from all the necessary data for applying the model. The model was run for three years using daily data from an existing nearby meteorological station. The predicted and measured soil erosion rates for a 7‐month period were 0.31 t and 0.18 t, respectively. Application of the model to each mapping unit showed the over‐riding importance of land use for sediment generation under the given climatic conditions. Bare land, occupying 5.5% of the watershed area, generated up to 69% of the total sediments estimated for the watershed. It is concluded that the pesera model can be used as a regional diagnostic tool under a range of soil, topographic and climatic conditions for identifying the best land use type and vegetation cover to protect hilly areas from soil erosion. The calculated overall root mean square error for the model is 0.06 t ha^?1 yr^?1, compared to a soil erosion rate of 0.04 t ha^?1 yr^?1, which can be tolerated for protecting the area for the sea turtle.