栀子苷的降糖作用和对PPARγ受体的激活

葡萄糖消耗试验表明,栀子苷能显著促进前脂肪细胞对葡萄糖的吸收。小鼠荷糖试验和四氧嘧啶致糖尿病小鼠血糖试验证实,栀子苷具有剂量依赖性降低小鼠餐后血糖及糖尿病高血糖的作用。通过基于PPARγ受体信号通路的报告基因诱导表达试验,发现栀子苷在体内外的降糖功效,可能与过氧化物酶增殖体激活受体γ的激活有关。     

利用昆虫性信息素防治杨树蛀干害虫技术试验

本文通过在四旁、林网释放这两种害虫的昆虫性信息素对害虫的生物学特性、防治效果进行了试验性研究。通过试验表明这两种害虫的性信息素在害虫监测预报方面应用价值极高、防治效果十分显著,其杀虫效果、防治成本等方面均优于常规方法,具有极大的推广价值。     

2006年特大干旱条件下森林植被对土壤水分的影响

2006年四川发生了百年一遇的特大干旱。于旱期结束末,调查了蓬安县境内两座丘陵-石马寨(栎柏混交林)和碉堡梁(人工柏木林)上、中、下坡林地和林带中耕地及坡脚农田的土壤含水量;调查了四川盆地丘陵区内5个县9个乡镇17个小流域有无农田防护林条件下,稻田开裂起始时间和裂缝宽;以及按照自然地理条件相近、森林覆盖率有较大差异的原则,调查了宣汉县和达县5对乡镇(森林覆盖率高的乡镇森林覆盖率为28.26%～46.85%,平均为37.24%,相对应的低的乡镇森林覆盖率为12.75%～26.74%,平均为18.64%)内不同台地的土壤含水量。结果表明,混交林林地的保水能力强于纯林林地,林地的保水能力强于耕地,农田防护林对水稻具有庇护作用,森林覆盖率高的地区土壤保水能力强于森林覆盖率低的地区土壤。在干旱期末坡上、中、下部位的0～40 cm土层中,栎柏混交林林地的含水量比柏木纯林林地高出3%～7%,林地的土壤含水量比林带中的耕地高出0.7%～2.1%;栎柏混交林带中的耕地含水量比柏木纯林带中的耕地高出3%～6%,石马寨的坡脚农田含水量比碉堡梁的高出5%;在干旱期末不同台地0～40 cm土层中,与森林覆盖率低的地区相比,森林覆盖率高的地区水稻田含水量高3%～7%,二台土高4%～8%,三台土高2%～8%;有农田防护林的农田土壤开裂时间比无农田防护林的推迟5 d～20 d,裂口宽平均窄0.1 cm～3.1 cm。     

6%抗坏血酸水剂对烟草的增产效果

分别从烟草生长发育、生长量和主要经济性状等方面研究6%抗坏血酸水剂对烟草的增产效果.结果表明,该药被烟叶吸收后,参与烟草生理生化全过程,促进烟草生长发育;烟草根系发达、植株变高、茎变粗、光合作用效率增强、叶绿素及抗坏血酸含量增加;上等烟率、橘黄烟率较对照提高20%～30%;改善烟叶的品质,平均增产20%,增收2250～3000元·hm-2.     

水产饲料的加工工艺

水产饲料大约需经过清理、粉碎、混合、调质、成形、颗粒后熟化、外涂七个或其中几个工段加工,才能成为到达养殖户手中的成品。在各个工段中,水产饲料生产对设备及加工参数有以下要求：     

Effects of Jiedu-Qingfei mixture on NF-κB and p38 MAPK pathways in lung tissues of rats with Mycoplasma pneumoniae infection

AIM: To observe the therapeutic effect of Jiedu-Qingfei mixture on Mycoplasma pneumoniae (MP)-infected rat lung tissues and to explore its mechanism. METHODS: SD rats (n=40) were randomly divided into 4 groups:blank control group, model group, Jiedu-Qingfei group and positive control group, with 10 rats in each group. The rats in experimental groups were slowly dripped with 1×10⁹ CFU/L MP solution into their nostrils for 4 d. One rat in each group was sacrificed for MP nucleic acid detection at the second day after inoculation, and the other rats were given gavage therapy. The rats in blank control group and model group were intragastrically given the same volume of normal saline, the rats in Jiedu-Qingfei group were given 8 mL/kg Jiedu-Qingfei mixture daily for 4 weeks, and the rats in psoitive control group were given dexmethasone sodium phosphate (0.5 mg·kg^-1·d^-1). After the experiment, the rats were killed. The serum and bronchoalveolar lavage fluid (BALF) were collected for detecting the levels of interleukin-12 (IL-12), IL-13 and TNF-α by ELISA. The right lung tissues were used for pathological observation and HE staining, while the left lung tissues were used to detect the expression of NF-κB p50, I-κBα and p38 mitogen-activated protein kinase (p38 MAPK) at mRNA and protein levels. RESULTS: The results of MP nucleic acid detection showed that all the rats except blank control group were MP nucleic acid positive, indicating that the rat model of MP infection was successfully established. On the 1st day of the treatment, the pathological scores of the lung tissues in model group and Jiedu-Qingfei group were significantly higher than those in blank control group (P<0.05). After treatment, the pathological scores of the lung tissues in mo-del group were significantly higher than those in blank control group and Jiedu-Qingfei group. The levels of IL-12 in the serum and BALF in model group were significantly lower than those in blank control group after MP infection (P<0.05), while those after treatment with Jiedu-Qingfei mixture were significantly higher than those in model group (P<0.05). The levels of IL-13 and TNF-α in the serum and BALF of MP-infected rats were increased significantly, while those after treatment with Jiedu-Qingfei mixture were significantly lower than those in model group (P<0.05). The mRNA expression levels of NF-κB p50 and p38 MAPK in model group were increased significantly (P<0.01). After treatment, the mRNA expression levels of NF-κB p50 and p38 MAPK were decreased significantly compared with model group (P<0.01). The mRNA expression level of I-κBα in model group was significantly lower than that in control group. After treatment, the mRNA expression of I-κBα in Jiedu-Qingfei group was significantly higher than that in model group (P<0.05). The protein levels of NF-κB p50 and p38 MAPK in the lung tissues of model group were significantly higher than those of blank control group. After treatment, the protein expression of NF-κB p50 and p38 MAPK was decreased significantly. The protein level of I-κBα in model group was significantly lower than that in blank control group, and after treatment with Jiedu-Qingfei mixture, the protein expression level of I-κBα was increased significantly (P<0.05). CONCLUSION: Jiedu-Qingfei mixture may attenuate lung tissue inflammation caused by MP through NF-κB and p38 MAPK pathways.     

新疆鄯善种子植物物种多样性及区系分析

文中在形态分类学研究的基础上,对鄯善野生种子植物物种多样性与区系地理进行了分析。鄯善共有种子植物48科177属,296种2亚种1变种。科的组成中,就属水平而言,寡属科与多属科构成科结构的主体,所含属数占74.01%,而就种水平而言,则大科和寡种科为主体,所含种数占81.94%;属的组成中,单种属和寡种属构成属结构的主体,所含种数占88.63%。该区新疆特有种与中国新疆仅分布种占26.09%,比例较高。生活型以地面芽植物与一、二年生植物为主;生态型以旱生植物与中生植物占较大优势,占种数的77.92%,与其区系的温带干旱性质相吻合。属的分布区类型表明,该区系地理成分多样,有11个分布类型和10个变型,温带性质明显,约占72.3%,以北温带为主。     

东北黑土区耕地利用效益空间差异及影响因素分析

耕地利用效益是评估区域耕地利用协调程度的必要前提,也是农业高效可持续发展的重要保障。本文以东北黑土区为研究区,依据耕地利用系统理论,构建“经济-社会-生态”效益框架,运用综合评价法、探索性数据分析法与地理探测器模型,从县域尺度分析2020年东北黑土区耕地利用效益空间差异和影响因素。结果显示：1）耕地利用综合效益、经济效益和生态效益整体上均呈现为由南部和中部平原地区向西部和北部低丘缓坡地形区减少趋势,社会效益则主要表现为由南部向北部递减的分布趋势。2）耕地利用综合效益整体上具有显著的集聚特征,高集聚主要在辽宁省南部以及北部黑龙江省县域范围分布,低集聚则集中分布在东部内蒙古和中部吉林省县域。3）从影响因素来看,黑土区耕地利用综合效益中气候、农业机械化程度和农作物复种指数是关键影响因素,且因素间的交互作用影响更加显著。为更好的提升黑土区耕地利用效益,需要把握乡村振兴协作、保护经营机制、农业综合措施和绿色技术创新等方面的促进作用,实现农业资源高效利用和可持续发展。     

一品红温室栽培目标花期关键技术

调节一品红在国庆节前开花,只有选择对感光不敏感的品种如千禧、旗帜,在常温下,每天光照时间为8h30min,时间长达70～80d,才能做到一品红在国庆节前供市。调节一品红在“五一”节前开花,补光时间要从第1年9月底至第2年2月底,时间长达5个月,每晚补光4h,每天光照时间长达15h左右,晚上光照强度400lx,夜间温度要保持在13℃;再经过1个星期的自然光照,又要进入遮光阶段,从3月7日开始,每天下午4:00至翌日早上8:00用黑色厚膜遮光,每天光照时间为8h,一直到全部苞片转红为止,时间长达50d。     

应用基因分型技术研究牛结核分枝杆菌分子流行病学

牛分枝杆菌是引起牛结核病(bTB)的病原体,侵染宿主广泛,可感染多种家畜和野生动物,对人类和动物健康构成巨大危害。随着多年不懈的研究,有关分枝杆菌分子流行病学的知识积累不断增加。作者对近年来应用新的基因分型技术研究牛结核分枝杆菌分子流行病学方面的一些研究进展。这些技术包限制性片段长度多态性分析(RFLP)、PCR介导的间隔区寡核苷酸分析(spoligotyping)、数目可变串联重复位点(VNTR)等。主要用于对人、牛、家畜以及野生动物的结核病分子流行病学进行分析和监测。另外,还利用一系列敏感的基于PCR的技术从分枝杆菌杆菌复合体(NTM)中对菌型进行鉴别。对分枝杆菌感染分子流行病学的全面了解,有助于对本病扩散传播机制的深入研究。可为制定科学防治结核病的措施做出贡献。