冬凤兰非共生萌发和低温离体保存（英文）

[Objective] The aim of the research was to establish asymbiotic germination and low-temperature in vitro conservation technique system of Cymbidium dayanum by using plant tissue culture technique to realize its rapid propagation and long-term conservation in vitro.[Method] With mature seeds of C.dayanum as explants,different media were selected to establish asymbiotic germination technique system.With protocorms as materials,conservation,resumptive proliferation and plant regeneration conditions were selected to establish low-temperature in vitro conservation technique system preliminarily.[Result] Mature seeds of C.dayanum could germinate after cultured 90 days on MS media as well as "Hyponex 1" media.The germination rate reached more than 98%.Protocorms inoculated in "Hyponex 1" media could be conserved continuously at 5 ℃ in dark for more than 18 months and the survival rate could reach 90%.Conserved protocorms could realize resumptive proliferation culture both on 1/2 MS and "Hyponex 1" media.The seedling-strengthening and rooting media were 1/2 MS media.[Conclusion] This research provided practical basis for in vitro conservation and rapid propagation of C.dayanum germplasm resource.     

Cloning and Sequence Analysis of Actin Gene from Rehmannia glutinosa

[Objective] The aim of this study is to clone and analyze the actin gene from Rehmannia glutinosa. [Method] Degenerate primers were designed according to the conserved regions of actin sequences of Rehmannia glutinosa and its similar species,RT-PCR was next conducted to amplify the actin gene from Rehmannia glutinosa. [Result] The amplified fragment is 724 bp and correspondingly 240 amino acids. The BLAST results indicate that the homology between the amplified fragment and other higher plants for actin gene sequences and amino acid are more than 80% and 90%,respectively,suggesting that the amplified fragment is the actin gene of Rehmannia glutinosa. [Conclusion] Phylogenetic analysis shows that the actin gene of Rehmannia glutinosa has an intimate genetic relationship with actin7 gene of Nicotiana tabacum.     

两种抗菌剂对油菜幼苗抗菌核病能力的影响

本试验以甘蓝型油菜渝黄1号为试材,分别用五种浓度的多菌灵溶液和五种浓度的百菌清溶液制成包衣剂,包衣处理油菜种子后,研究其对油菜幼苗抗菌核病能力的影响,并分析了不同浓度和不同种类的药品对油菜种子种子的萌发指数和幼苗的根茎长度、根系活力,电导率的影响。试验结果表明,0．45—0．71g/L多菌灵和0．83—1．5g／L百菌清都对提高甘蓝型油菜幼苗抗菌核病能力有作用,但综合比较得出：0．45—0．56g／L效果最好,它既能提高幼苗的抗病能力又不会对幼苗生长产生不利影响。     

蒙脱土/木材复合材料应力松弛性能的初步研究

以酚醛树脂为中间介质,将蒙脱土引入木材,制备蒙脱土/木材复合材料.通过研究该复合材料的应力松弛性能并与木材比较发现,蒙脱土/木材复合材料的抗应力松弛性能比木材明显增强.当蒙脱土含量为酚醛树脂固含量的5%时,所制备的复合材料的抗应力松弛性能最好.复合材料的抗应力松弛能力对温度较敏感.     

Caratheódory定理与热力学第二定律

在重温Caratheódory定理的基本理论结果的基础上,着重阐明了该定理的普适性,从而判明了Clausius热力学第二定律可适用的条件,以及负能态热力学存在的合理性.     

云贵高原干季室内热环境参数关系研究

基于我国住宅建筑室内热环境研究的不足,以曲靖师范学院一自然通风房间为对象,对其室内温度、湿度、风速、墙面温度、地板温度、天花板温度、室外环境温度进行了为期半年的实地测试,对室内空气平均温度、平均辐射温度、PMV(predicted mean vote)值和PPD(predicted percentage of dissatisfaction)值进行了计算.为便于快捷预测和调控云贵高原干季室内热环境,利用线性回归分析法对室内外主要参数间的关系进行了讨论,获得了一些相关性较好的回归方程,在相似气候下对热环境预测和设计可供参考.     

规模化猪场猪繁殖与呼吸综合征感染混合细菌研究

规模化猪场爆发急性高热病,采集死猪肺脏病料和血清5份用RT-PCR方法进行PRRSVNSP2基因扩增,3份为猪繁殖与呼吸综合征阳性,编号为scwhn01,scwhn02和scwhn03.对scwhn01 NSP2基因进行克隆和序列测定分析,有30个氨基酸的不连续缺失,与VR2332,JXA1,BJ-4,HuN4,CH-1a,HB-1(sh)2002和HB-2(sh)2002的NSP2的同源率在74.2%~91.0%之间,并从病料中分离到2株细菌,编号分别为SNC0901和SNC0902,均使被攻毒小鼠死亡.16S rDNA测序并在Genbank中blast鉴定为副猪嗜血杆菌和大肠杆菌.对所分离的细菌进行17种抗生素药敏实验,对头孢噻呋、头孢噻吩等敏感;对克林霉素、阿莫西林/棒酸等中度敏感;对荷兰、林可霉素、丁胺卡那霉素和土霉素等耐药.表明该猪场因高致病性蓝耳病病毒混合感染副猪嗜血杆菌、大肠杆菌导致猪大量死亡.     

二卤代荧光素阿曲库铵体系的吸收光谱及其分析应用

在弱酸性Britton-Robinson缓冲溶液中,阿曲库铵(AB)与二氯荧光素(DCF)、二溴荧光素(DBF)、二碘荧光素(DIF)形成离子缔合物,引起这3种染料的最大吸收峰明显降低,并在532 nm(DCF体系)、540 nm(DBF体系)和551 nm(DIF体系)处出现新的吸收峰.在一定范围内,AB浓度与吸光度的改变值(ΔA)成良好的线性关系,不同体系的摩尔吸光系数(ε)在9.95×103~1.22×105L/(mol.cm)之间.据此,提出了测定AB的新分光光度法,并考察了体系的光谱特征、适宜的反应条件和共存物质的影响.将该方法用于尿样中AB的测定,回收率在97.6%~104.4%之间.     

小麦秸秆储存机理研究

研究了小麦采用全喂入和半喂入两种方式收获后,再对田间麦秸采用打捆机进行捡拾收集打捆,并将麦秸捆储存于遮雨通风自然环境和环境温度相同、相对湿度恒定环境的两种环境中,跟踪测试了麦秸捆的体积密度、含水率、芯部温度以及热值等参数,探索了麦秸捆储存期间内在因素相互影响的关系.研究结果表明:无论是正常年份,还是相对湿度较大的特殊年份,麦秸捆储存期间芯部温度均没有超出安全水平线,储存前后绝干热值没有明显影响,麦秸捆均可安全储存并作能源利用.     

MAPP对麦秸纤维-聚丙烯复合材料热力学性能的影响

以麦秸纤维为增强材料、聚丙烯为基体物质、马来酸酐接枝聚丙烯（MAPP）为改性剂,制备麦秸纤维-聚丙烯复合材料。利用DMA、DSC、TG和SEM,探讨了MAPP的添加量（质量百分比1%、2%、5%、10%）和麦秸纤维形态（9、9～28、28～35、35目）对麦秸纤维 聚丙烯复合材料的热力学性能和结晶性能的影响。结果表明:①当MAPP的添加量为2%时,麦秸纤维-聚丙烯复合材料的储能弹性模量减小;当MAPP的添加量增加到5%、10%时,复合材料的储能弹性模量增加。②在麦秸纤维-聚丙烯体系内添加MAPP后,麦秸纤维 聚丙烯复合材料的结晶温度提高约1℃,结晶度增加了4%～8%;麦秸纤维以28～35目的形态作为聚丙烯基体的增强材料时,其复合材料的结晶温度为122.7℃,结晶率达到45.8%。③麦秸纤维-聚丙烯复合材料的热分解峰温分别为355和460℃。④麦秸纤维以纤维束的形态分布在基体聚丙烯中起增强作用,在整个体系内,麦秸纤维局部团聚且断裂明显。添加MAPP后,有利于基体物质在麦秸纤维表面的均匀覆盖。