光温调控对切花菊"优香"养分利用及品质的影响

光照与温度影响着切花菊的花芽分化进程,是切花菊畸形花形成的主要环境因子。如何在夏季高温期有效调控设施内的光照与温度对减少畸形花率、提高产品出成率及保鲜期具有重要意义。现以解决企业关键技术需求为出发点,以夏秋菊品种"优香"为试材,在设施生产条件下,利用温湿度自动监控系统、光温调控设施进行了遮光、降温处理,旨在为高温期的切花菊生产光温调控技术提供支撑。结果表明:采收期遮光与降温处理(8 127kg/hm~2)与未调控处理(6 270kg/hm~2)相比,地上部干物质累积量差异不显著,但切花菊的吸磷量显著增加了7.1kg/hm~2,氮和钾的吸收量差异不显著。经光温调控后,切花菊畸形花率由未调控下的42.5%降低至20.1%,外观品质基本一致,清水瓶插寿命延长了2~3d。基于自动温湿度监控系统的切花菊设施生产光温调控技术对切花菊外观品质影响不大,且在未影响切花菊正常干物质累积及养分利用的前提下,可有效降低花朵畸形花率、提高保鲜期和优级花的产出。     

中间锦鸡儿花水浸提液对三种作物种子萌发及幼苗生长的化感作用

以中间锦鸡儿和玉米、苏丹草、高丹草种子为试材,以蒸馏水为对照,采用培养皿滤纸法,研究了不同梯度(0.5、1.0、5.0、10.0、25.0mg·mL~(-1))中间锦鸡儿花水浸提液对3种受体作物种子萌发及幼苗生长的影响,以期对农林复合系统生产和构建提供理论依据和有益参考。结果表明:随着中间锦鸡儿花水浸提液质量浓度的增加,受体作物玉米、苏丹草、高丹草种子的平均发芽率和发芽指数均表现"低浓度促进,高浓度抑制"效应,但不同质量浓度水浸提液对3种农作物种子发芽的影响有差异性,在质量浓度为1.0mg·mL~(-1)时,3种农作物的种子相对发芽率间无显著差异(P0.05),除玉米外,另外2种受体作物种子的平均发芽率均表现为促进作用,且对高丹草的促进作用最大,RI=13.70%;在高浓度浸提液(C≥5.0mg·mL~(-1))处理下3种受体作物种子的发芽指数与对照均表现显著差异(P0.05)。玉米、苏丹草和高丹草的胚根、胚芽对中间锦鸡儿花水浸提液的化感效应均表现为"浓度效应";3种受体作物的苗高随着浸提液处理浓度的增大表现为"低促高抑",高浓度的浸提液对高丹草幼苗高度的抑制作用最强。花水浸提液对苏丹草和高丹草的幼苗干重化感效应表现为"低促高抑"效应,而对玉米幼苗干重表现为"高浓度抑制"效应。中间锦鸡儿花水浸提液对3种受体作物的化感作用综合效应大小依次为高丹草玉米苏丹草。     

“西育1号”芹菜周年栽培技术

"西育1号"芹菜新品种于2009年10月通过陕西省农作物品种审定委员会登记。经过在西安三桥农场、西安市现代农业科技展示中心多年的多茬口栽培试验以及高陵、阎良、周至等区县的试验示范,总结形成了一套适合关中地区"西育1号"芹菜的优质高效周年栽培技术,以期为该品种的推广提供技术指导。     

复配材料超声处理对化肥控失效果的影响

利用超声波对化肥控失材料(LCM)进行修饰改性,结果表明对硫酸铵的控失效果随处理时间呈现先升高然后降低的规律,峰值出现在5min.本文对其机理进行初步探讨.     

Clinical characteristics and mode of inheritance of familial focal seizures in Standard Poodles

OBJECTIVE: To determine clinical characteristics and mode of inheritance of seizures in a family of Standard Poodles. DESIGN: Case series. ANIMALS: 90 Standard Poodles descended from the same maternal bloodline (30 with probable idiopathic epilepsy [PIE] and 60 without any history of seizures). PROCEDURES: Researchers contacted owners to determine whether dogs had ever had any seizures and, if so, the nature of any such seizures and any potential underlying causes. Dogs were considered to have PIE if they were between 6 months and 7.5 years old at the time of seizure onset and had no evidence of any underlying cause. To determine the mode of inheritance, segregation analyses were designed to allow the family to be analyzed as a whole, as opposed to as nuclear families. Competing models of inheritance were compared statistically for their ability to explain the data. RESULTS: Of the dogs with PIE, 28 (93%) had focal onset seizures with or without secondary generalization. Median age of onset was 3.7 years; 6 dogs were > 5 years old at the onset of seizures. Segregation analyses strongly suggested that PIE was inherited as a simple recessive autosomal trait with complete or almost complete penetrance. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that in this family of Standard Poodles, PIE was inherited as a simple recessive autosomal trait with complete or almost complete penetrance. Seizures often had focal, as opposed to generalized, onsets, and it was not uncommon for seizures to begin after 5 years of age.     

Purification and characterization of feline ghrelin and its possible role

Ghrelin, a novel 28-amino acid peptide with an n-octanoyl modification at Ser3, has been isolated from rat and human stomach as an endogenous ligand for the growth hormone secretagogue receptor. Here, we purified feline ghrelin and examined its possible physiological role in cats. The major active form of feline ghrelin is a 28-amino acid peptide octanoylated (C8:0) at Ser3; except for one amino acid residue replacement, this structure is identical to those of rat and human ghrelins. However, much structural divergence in peptide length and fatty acid modification was observed in feline ghrelin: peptides consisting of 27 or 26 amino acids lacking Gln14 and/or Arg28 were found, and the third serine residue was modified by octanoic acid (C8:0), decanoic acid (10:0), or unsaturated fatty acids (C8:1, C10:1 and C10:2). In agreement with the structural divergence, two kinds of cDNA with different lengths were isolated. Administration of synthetic rat ghrelin increased plasma growth hormone levels in cats, with a potency similar to that in rat or human. Plasma levels of ghrelin in cats increased approximately 2.5-fold after fasting. The present study indicates the existence of structural divergence in feline ghrelin and suggests that, as in other animals, ghrelin may play important roles in GH release and feeding in cats.     

Evaluation of immunogenicity and protective efficacy of Actinobacillus pleuropneumoniae HB04C(-) mutant lacking a drug resistance marker in the pigs

Previously, we reported the construction and characterization of a genetically defined Actinobacillus pleuropneumoniae (A. pleuropneumoniae) apxIIC gene mutant, HB04C(-), which conferred protection to mice against infection with A. pleuropneumoniae. In this study, we further evaluated HB04C(-) for safety and its ability to elicit protective immunity in pigs. It was demonstrated that a dose of 2 x 10(8) CFU HB04C(-) was safe to the pigs via intranasal or intramuscular injection. Immunization with a dose of 2 x 10(8) HB04C(-) by both intranasal and intramuscular routine could yield equal protective efficacy and elicited significant protection against experiment challenge with homologous or heterologous serotypes of a virulent A. pleuropneumonia. Taken together, HB04C(-) might serve as a promising vaccine candidate against infection with A. pleuropneumoniae.     

Streptococcus suis infections in humans: the Chinese experience and the situation in North America

Infections caused by Streptococcus suis are considered a global problem in the swine industry. In this animal species, S. suis is associated with septicemia, meningitis, endocarditis, arthritis and, occasionally, other infections. Moreover, it is an agent of zoonosis that afflicts people in close contact with infected pigs or pork-derived products. Although sporadic cases of S. suis infection in humans have been reported, a large outbreak due to S. suis serotype 2 emerged in the summer of 2005 in Sichuan, China. A similar outbreak was observed in another Chinese province in 1998. Symptoms reported in these two outbreaks include high fever, malaise, nausea and vomiting, followed by nervous symptoms, subcutaneous hemorrhage, septic shock and coma in severe cases. The increased severity of S. suis infections in humans, such as a shorter incubation time, more rapid disease progression and higher rate of mortality, underscores the critical need to better understand the factors associated with pathogenesis of S. suis infection. From the 35 capsular serotypes currently known, serotype 2 is considered the most virulent and frequently isolated in both swine and humans. Here, we review the epidemiological, clinical and immunopathological features of S. suis infection in humans.     

Hypertrophic osteopathy associated with disseminated metastases of renal cell carcinoma in the dog: a case report

A 6-year-old, mixed breed, intact male dog showed signs of left carpal joint swelling and weakness of the forelimbs one month before presentation. The symptoms gradually progressed to bilateral carpal and tarsal joint swelling and tetraparalysis. There were a number of radiographically identified lytic-proliferative bone lesions noted on the axial skeleton. Hypertrophic osteopathy of the metacarpi and all distal long bones was also evident. Because of the deteriorating quality of life and guarded prognosis, the patient was euthanized and a complete necropsy was performed. Renal cell carcinoma, with metastasis to the lung, thoracic vertebrae, ribs, and the right adrenal gland, was diagnosed. To our knowledge, renal cell carcinoma with bone metastases and hypertrophic osteopathy has not been reported in dogs.     

Surgical outcome and ocular complications of evisceration and intraocular prosthesis implantation in dogs with end stage glaucoma: a review of 20 cases

Surgical outcome and complications following evisceration and implantation of intraocular silicone prosthesis (ISP) in 20 canine eyes with end-stage glaucoma were analyzed, including clinical signs, complications, cosmetic appearance, and owners' responses. The mean postoperative follow-up time was 312 days. Major short-term surgical complications were central corneal ulceration (6/20) and infections (3/20), while long-term complication was keratoconjunctivitis sicca (KCS) (2/20). All patient owners (100%) showed satisfied with the surgical outcome and postoperative cosmetic effect including 85% of the owners gave an excellent or good rating of satisfaction. No more medication needed for long term control except the KCS cases. With careful case selection and post-operative care, evisceration with ISP implantation proves a good and safe surgical procedure with minimal complications for end stage glaucoma.